GpDSR7, a Novel E3 Ubiquitin Ligase Gene in Grimmia pilifera Is Involved in Tolerance to Drought Stress in Arabidopsis

Li, Mengmeng; Li, Yihao; Zhao, Junyi; Hai, Liu; Jia, Shenghua; Li, Jie; Zhao, Heping; Han, Shengcheng; Wang, Yingdian

doi:10.1371/journal.pone.0155455

Cited by 10 publications

(5 citation statements)

References 45 publications

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“…In recent years, plant RING E3 ligases have received much attention by the plant scientists due to their putative involvement in diverse defense responses to abiotic and biotic stresses. In particular, plant RING E3 ligases impart broad-spectrum resistance to Alternaria alternata ( Kabbage et al, 2010 ), salt stress ( Kabbage et al, 2010 ), drought stress ( Li et al, 2016 ) and Heterodera schachtii infection ( Hewezi et al, 2016 ). They also play a key role in ABA and ethylene signaling by regulating ABA-mediated seed germination and cell death, and ethylene-mediated fruit ripening.…”

Section: Discussionmentioning

confidence: 99%

Overexpression of E3 Ubiquitin Ligase Gene AdBiL Contributes to Resistance against Chilling Stress and Leaf Mold Disease in Tomato

et al. 2017

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Ubiquitination is a common regulatory mechanism, playing a critical role in diverse cellular and developmental processes in eukaryotes. However, a few reports on the functional correlation between E3 ubiquitin ligases and reactive oxygen species (ROS) or reactive nitrogen species (RNS) metabolism in response to stress are currently available in plants. In the present study, the E3 ubiquitin ligase gene AdBiL (Adi3 Binding E3 Ligase) was introduced into tomato line Ailsa Craig via Agrobacterium-mediated method. Transgenic lines were confirmed for integration into the tomato genome using PCR. Transcription of AdBiL in various transgenic lines was determined using real-time PCR. Evaluation of stress tolerance showed that T1 generation of transgenic tomato lines showed only mild symptoms of chilling injury as evident by higher biomass accumulation and chlorophyll content than those of non-transformed plants. Compared with wild-type plants, the contents of AsA, AsA/DHA, GSH and the activity of GaILDH, γ-GCS and GSNOR were increased, while H2O2, O2.−, MDA, NO, SNOs, and GSNO accumulations were significantly decreased in AdBiL overexpressing plants in response to chilling stress. Furthermore, transgenic tomato plants overexpressing AdBiL showed higher activities of enzymes such as G6PDH, 6PGDH, NADP-ICDH, and NADP-ME involved in pentose phosphate pathway (PPP). The transgenic tomato plants also exhibited an enhanced tolerance against the necrotrophic fungus Cladosporium fulvum. Tyrosine nitration protein was activated in the plants infected with leaf mold disease, while the inhibition could be recovered in AdBiL gene overexpressing lines. Taken together, our results revealed a possible physiological role of AdBiL in the activation of the key enzymes of AsA–GSH cycle, PPP and down-regulation of GSNO reductase, thereby reducing oxidative and nitrosative stress in plants. This study demonstrates an optimized transgenic strategy using AdBiL gene for crop improvement against biotic and abiotic stress factors.

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Section: Discussionmentioning

confidence: 99%

Overexpression of E3 Ubiquitin Ligase Gene AdBiL Contributes to Resistance against Chilling Stress and Leaf Mold Disease in Tomato

et al. 2017

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“…Gene replication can arise from fragment replication, tandem replication, transposable events and even whole genome replication, which not only provide the evolutionary potential for species to produce new functional traits but also are a main driving force for species differentiation (Moore & Purugganan, 2003;Ren et al, 2018). In the identification of gene families from many species, gene replication events have proven instrumental in their expansion (Wang et al, 2016). We observed that some grapevine VyRCHCscorrespond to one or more intraspecific homologous genes, thus indicating VyRCHC gene family's amplification in this plant may have been caused by gene duplication.…”

Section: Discussionmentioning

confidence: 88%

Genome-Wide Identification of Grapevine's C3H2C3 Type RING E3 Ubiquitin Ligases Family Reveals VyRCHC114, which Confers Tolerance to Drought Stresses

Yang

et al. 2020

Preprint

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RING is one of the largest E3 ubiquitin ligase families, playing an important role in plants' development and growth and their biotic and abiotic stress responses. The C3H2C3 type is the largest subfamily of RING. Here, 143 C3H2C3-type RING genes (RCHCs) were discovered from the grapevine genome and separated into groups (I˜XI) according to their phylogenetic analysis, with these genes named according to their positions on chromosomes. Gene replication analysis showed that 26 group segmental duplications and 14 tandem duplications of VyRCHCs were identified. All VyRCHCs have introns, VyRCHC29 has a maximum of 19 introns and 96 VyRCHCs have introns of 1 to 3. ARE, ABRE and O2 site Cis-acting elements very conservative in the promoter of VyRCHCs, with a large number. The expression profiles of eight DEGs in RNA-Seq after drought stress were similar to those in qRT-PCR analysis. The in vitro ubiquitin experiment showed that VyRCHC114 had E3 ubiquitin ligase activity, while Arabidopsis thaliana overexpressing VyRCHC114 had stronger tolerance of drought stress than the control. Valuable new information on the evolution of grapevine RCHC family genes and its relevance for studying the functional characteristics of grapevine VyRCHC114 genes under drought stress emerged from this research. Recent studies have shown that the RING E3 ubiquitin ligase plays a key role in biological stress responses of plants. For example, in tobacco,NtRFP1 encodes the C3-H-C4 type RING E3 ligase, which can diminish the

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“…In addition, E3 ubiquitin ligase atrzf1 mutation increased the proline content of Arabidopsis and improved drought tolerance [42]. GpDSR7 encodes an E3 ubiquitin ligase, which overexpressed in Arabidopsis increased its tolerance to drought stress [43]. In our study, focused on genes which were significantly up-regulated or down-regulated at four time periods during the drought treatment according to previous screening methods [44], as they more likely to play a key role in grapevine's drought stress response (Fig.…”

Section: Discussionmentioning

confidence: 99%

Identification of C3H2C3-type RING E3 ubiquitin ligase in grapevine and characterization of drought resistance function of VyRCHC114

Yang

et al. 2021

BMC Plant Biol

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Background RING is one of the largest E3 ubiquitin ligase families and C3H2C3 type is the largest subfamily of RING, which plays an important role in plant growth and development, and growth and responses to biotic and abiotic stresses. Results A total of 143 RING C3H2C3-type genes (RCHCs) were discovered from the grapevine genome and separated into groups (I-XI) according to their phylogenetic analysis, and these genes named according to their positions on chromosomes. Gene replication analysis showed that tandem duplications play a predominant role in the expansion of VvRCHCs family together. Structural analysis showed that most VvRCHCs (67.13 %) had no more than 2 introns, while genes clustered together based on phylogenetic trees had similar motifs and evolutionarily conserved structures. Cis-acting element analysis showed the diversity of VvRCHCs regulation. The expression profiles of eight DEGs in RNA-Seq after drought stress were like the results of qRT-PCR analysis. In vitro ubiquitin experiment showed that VyRCHC114 had E3 ubiquitin ligase activity, overexpression of VyRCHC114 in Arabidopsis improved drought tolerance. Moreover, the transgenic plant survival rate increased by 30 %, accompanied by electrolyte leakage, chlorophyll content and the activities of SOD, POD, APX and CAT were changed. The quantitative expression of AtCOR15a, AtRD29A, AtERD15 and AtP5CS1 showed that they participated in the response to drought stress may be regulated by the expression of VyRCHC114. Conclusions This study provides valuable new information for the evolution of grapevine RCHCs and its relevance for studying the functional characteristics of grapevine VyRCHC114 genes under drought stress.

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GpDSR7, a Novel E3 Ubiquitin Ligase Gene in Grimmia pilifera Is Involved in Tolerance to Drought Stress in Arabidopsis

Cited by 10 publications

References 45 publications

Overexpression of E3 Ubiquitin Ligase Gene AdBiL Contributes to Resistance against Chilling Stress and Leaf Mold Disease in Tomato

Overexpression of E3 Ubiquitin Ligase Gene AdBiL Contributes to Resistance against Chilling Stress and Leaf Mold Disease in Tomato

Genome-Wide Identification of Grapevine's C3H2C3 Type RING E3 Ubiquitin Ligases Family Reveals VyRCHC114, which Confers Tolerance to Drought Stresses

Identification of C3H2C3-type RING E3 ubiquitin ligase in grapevine and characterization of drought resistance function of VyRCHC114

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