GP5 Protein-based ELISA for the Detection of PRRSV Antibodies

Abstract

Porcine reproductive and respiratory syndrome virus (PRRSV) is an important swine pathogen, causing huge economic losses each year worldwide. Immunization with vaccines containing the glycoprotein 5 (GP5) of PRRSV is the main measure to induce neutralizing antibodies and control the disease. Here, we developed a GP5 protein-based ELISA for detecting antibodies against PRRSV. The overall yield of purified GP5 in E. coli flask culture was more than 45 mg/L cell culture. Western blot and IFA indicated that the GP5 protein was highly immunogenic. After optimization and validation with IDEXX PRRS using 566 clinical sera, the DSN, DSP, and accuracy of GP5-ELISA were 81.39%, 75.96%, and 80.39%, respectively. Besides, GP5-ELISA is highly specific, showing no cross-reactions with sera against other important swine pathogens. Hence, GP5 is a good diagnostic antigen and the GP5 protein-based ELISA has the potential to be used in the field.

Polish Journal of Veterinary Sciences

“…The specificity of the MAbs was examined using enzyme-linked immunosorbent assay (ELISA). Recombinant E2 protein of classical swine fever virus (CSFV), GP5 protein of porcine reproductive and respiratory syndrome virus (PRRSV), gB protein of pseudorabies virus (PRV), VP1 protein of foot and mouth disease virus (FMDV) and S1 protein of porcine epidemic diarrhea virus (PEDV) were coated onto 96-well plates to determine the specificity of the MAbs in ELISA as previously described (Wang et al 2016.…”

Graphene Oxide-Sensitized Surface Plasmon Resonance Biosensor of Porcine Reproductive and Respiratory Syndrome Virus

“…PRRSV has caused huge economic losses in the global pig industry [14], and its high mortality, strong transmission, multiple transmission routes, and strong mutagenicity [15] have had a significant impact on the pig industry in China. Traditional PRRSV detection techniques include quantitative reverse transcription polymerase chain reaction (qRT-PCR), enzyme-linked immunosorbent assay (ELISA), immunohistochemistry, antibody detection, and so on [16][17][18]. These methods are time-consuming and virus sample damage treatment.…”