GOx-encapsulated iron-phenolic networks power catalytic cascade to eradicate bacterial biofilms

Piao, Yinzi; Yu, Qi; Hu, Xiaowen; Wang, Yaran; Li, Yuanfeng; Zhou, Tieli; Shi, Linqi; Liu, Yong; Zhou, Chaoyang

doi:10.1016/j.jconrel.2022.10.013

Cited by 17 publications

(17 citation statements)

References 44 publications

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“…Furthermore, we investigated the efficacy of Lipid-CIP assemblies in preventing biofilm formation and eradicating mature biofilms . A biofilm is a cluster of bacteria in their self-produced extracellular polymeric substances and accounts for over 80% of chronic infections in patients .…”

Section: Resultsmentioning

confidence: 99%

“…58,59 Furthermore, we investigated the efficacy of Lipid-CIP assemblies in preventing biofilm formation and eradicating mature biofilms. 60 A biofilm is a cluster of bacteria in their selfproduced extracellular polymeric substances and accounts for over 80% of chronic infections in patients. 61 Pristine Lipid showed little ability to prevent the formation of biofilms, and considerable biofilms formed on the surfaces pretreated with pristine Lipid (Figures 3a,b).…”

Section: Resultsmentioning

confidence: 99%

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Lipid Prodrug Nanoassemblies via Dynamic Covalent Boronates

Ding

Piao

et al. 2023

ACS Nano

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Prodrug nanoassemblies combine the advantages of prodrug and nanomedicines, offering great potential in targeting the lesion sites and specific on-demand drug release, maximizing the therapeutic performance while minimizing their side effects. However, there is still lacking a facile pathway to prepare the lipid prodrug nanoassemblies (LPNAs). Herein, we report the LPNAs via the dynamic covalent boronate between catechol and boronic acid. The resulting LPNAs possess properties like drug loading in a dynamic covalent manner, charge reversal in an acidic microenvironment, and specific drug release at an acidic and/or oxidative microenvironment. Our methodology enables the encapsulation and delivery of three model drugs: ciprofloxacin, bortezomib, and miconazole. Moreover, the LPNAs are often more efficient in eradicating pathogens or cancer cells than their free counterparts, both in vitro and in vivo. Together, our LPNAs with intriguing properties may boost the development of drug delivery and facilitate their clinical applications.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Lipid Prodrug Nanoassemblies via Dynamic Covalent Boronates

Ding

Piao

et al. 2023

ACS Nano

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“…Bacterial infections, especially those caused by multidrug resistant pathogens, have significantly threatened human health. , In nature, enzymes are widely involved in combating the invaded pathogens, especially in the Kingdoms Plantae and Animalia. , For example, lysozymes can induce bacterial lysis via cleavage of the peptidoglycans on the bacterial cell walls. − Enzymatic cascade reactions, powered by NADPH oxidase, superoxide dismutase (SOD), and myeloperoxidase (MPO), can efficiently produce highly oxidative HOCl • to damage the bacterial cell membrane. , Although natural enzymes are selective and efficient in catalysis, they also suffer from poor thermal stability, susceptibility to proteases and pH change, and high cost-effectiveness ratio. − To address the intrinsic shortcomings of natural enzymes, one promising strategy is to develop artificial enzyme mimics (AEMs). − In principle, most of these AEMs possess peroxidase and oxidase activity that can boost the generation of reactive oxygen species (ROS) to damage the cell membrane, DNA, and biomacromolecules. , Several metal oxides, ions, and carbon-based nanomaterials have been developed and used as AEMs. ,, In most cases, these AEMs have a single function, used individually to catalyze reactions or in combination to achieve cascades. , Therefore, there is an urgent need to develop enzyme mimics with multiple functions to enable cascades in one system and subsequent biomedical applications.…”

Section: Introductionmentioning

confidence: 99%

Single Copper Atom Photocatalyst Powers an Integrated Catalytic Cascade for Drug-Resistant Bacteria Elimination

Wang

et al. 2023

ACS Nano

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To address the issue posed by drug-resistant bacteria and inspired by natural antimicrobial enzymes, we report the atomically doped copper on guanine-derived nanosheets (G–Cu) that possess the integrated catalytic cascade property of glucose oxidase and peroxidase, yielding free radicals to eliminate drug-resistant bacteria upon light irradiation. Density functional theory calculations demonstrate that copper could notably promote oxygen activation and H2O2 splitting on the G–Cu complexes. Further all-atom simulation and experimental data indicate that the lysis of bacteria is mainly induced by cell membrane damage and the elevation of intracellular reactive oxygen species. Lastly, the G–Cu complexes efficiently eliminate the staphylococci in the infected wounds and accelerate their closure in a murine model, with negligible side effects on the normal tissues. Therefore, our G–Cu complexes may provide an efficient nonantibiotic alternative to the current treatments for bacterial infections.

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“…[ 11,14 ] Also, nano‐networks or porous structures could facilitate the diffusion of substrates and products, further improving the enzymatic efficiency. For instance, single‐atom nanoparticles, [ 32–34 ] metal‐phenolic nano‐networks, [ 35–40 ] metal‐organic frameworks, [ 41,42 ] and covalent‐organic frameworks [ 43,44 ] are well‐known examples of peroxidase‐like systems with relatively high enzymatic activities. However, most enzyme mimics need laborious preparation procedures and modification steps, resulting in low reproducibility for clinical translation.…”

Section: Introductionmentioning

confidence: 99%

Neighboring Carboxylic Acid Boosts Peroxidase‐Like Property of Metal‐Phenolic Nano‐Networks in Eradicating Streptococcus mutans Biofilms

Wang

Zhou

et al. 2022

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Developing nature‐inspired nanomaterials with enzymatic activity is essential in combating bacterial biofilms. Here, it is reported that incorporating the carboxylic acid in phenolic/Fe nano‐networks can efficiently manipulate their peroxidase‐like activity via the acidic microenvironment and neighboring effect of the carboxyl group. The optimal gallic acid/Fe (GA/Fe) nano‐networks demonstrate highly enzymatic activity in catalyzing H2O2 into oxidative radicals, damaging the cell membrane and extracellular DNA in Streptococcus mutans biofilms. Theoretical calculation suggests that the neighboring carboxyl group can aid the H2O2 adsorption, free radical generation, and catalyst reactivation, resulting in superb catalytic efficiency. Further all‐atom simulation suggests the peroxidation of lipids can increase the cell membrane fluidity and permeability. Also, GA/Fe nano‐networks show great potential in inhibiting tooth decay and treating other biofilm‐associated diseases without affecting the commensal oral flora. This strategy provides a facile and scale‐up way to prepare the enzyme‐like materials and manipulate their enzymatic activity for biomedical applications.

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GOx-encapsulated iron-phenolic networks power catalytic cascade to eradicate bacterial biofilms

Cited by 17 publications

References 44 publications

Lipid Prodrug Nanoassemblies via Dynamic Covalent Boronates

Lipid Prodrug Nanoassemblies via Dynamic Covalent Boronates

Single Copper Atom Photocatalyst Powers an Integrated Catalytic Cascade for Drug-Resistant Bacteria Elimination

Neighboring Carboxylic Acid Boosts Peroxidase‐Like Property of Metal‐Phenolic Nano‐Networks in Eradicating Streptococcus mutans Biofilms

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