Governor Vessel Electro-Acupuncture Promotes the Intrinsic Growth Ability of Spinal Neurons through Activating Calcitonin Gene-Related Peptide/α-Calcium/Calmodulin-Dependent Protein Kinase/Neurotrophin-3 Pathway after Spinal Cord Injury

Xu, Hongmei; Yang, Yang; Deng, Qingwen; Zhang, Bao-Bao; Ruan, Jing-Wen; Jin, Hui; Wang, Junhua; Ren, Jia-Le; Jiang, Bin; Sun, Jia-Hui; Zeng, Yuan-Shan; Ding, Ying

doi:10.1089/neu.2020.7155

Cited by 21 publications

(38 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Another finding from this study was the importance of the NT‐3 level in the injured spinal cord, which was markedly augmented by EA treatment 11,25 . Our recent study has also verified that EA could stimulate the spinal nerve branches of the dorsal root ganglion (DRG) cells to increase the release of calcitonin gene‐related peptide (CGRP) in the injured spinal cord, and then trigger the synthesis and secretion of NT‐3 in spinal neurons by activating the CGRP/RAMP1/αCaMKII pathway and the intrinsic growth ability of spinal neurons after SCI 32 . Additionally, NT‐3 can promote the survival and differentiation of neurons necessitated for the development of descending motoneuron pathways 33 …”

Section: Discussionmentioning

confidence: 79%

Electroacupuncture facilitates the integration of a grafted TrkC‐modified mesenchymal stem cell‐derived neural network into transected spinal cord in rats via increasing neurotrophin‐3

Yang

Deng

et al. 2021

CNS Neurosci Ther

Self Cite

View full text Add to dashboard Cite

Aims This study was aimed to investigate whether electroacupuncture (EA) would increase the secretion of neurotrophin‐3 (NT‐3) from injured spinal cord tissue, and, if so, whether the increased NT‐3 would promote the survival, differentiation, and migration of grafted tyrosine kinase C (TrkC)‐modified mesenchymal stem cell (MSC)‐derived neural network cells. We next sought to determine if the latter would integrate with the host spinal cord neural circuit to improve the neurological function of injured spinal cord. Methods After NT‐3‐modified Schwann cells (SCs) and TrkC‐modified MSCs were co‐cultured in a gelatin sponge scaffold for 14 days, the MSCs differentiated into neuron‐like cells that formed a MSC‐derived neural network (MN) implant. On this basis, we combined the MN implantation with EA in a rat model of spinal cord injury (SCI) and performed immunohistochemical staining, neural tracing, electrophysiology, and behavioral testing after 8 weeks. Results Electroacupuncture application enhanced the production of endogenous NT‐3 in damaged spinal cord tissues. The increase in local NT‐3 production promoted the survival, migration, and maintenance of the grafted MN, which expressed NT‐3 high‐affinity TrkC. The combination of MN implantation and EA application improved cortical motor‐evoked potential relay and facilitated the locomotor performance of the paralyzed hindlimb compared with those of controls. These results suggest that the MN was better integrated into the host spinal cord neural network after EA treatment compared with control treatment. Conclusions Electroacupuncture as an adjuvant therapy for TrkC‐modified MSC‐derived MN, acted by increasing the local production of NT‐3, which accelerated neural network reconstruction and restoration of spinal cord function following SCI.

show abstract

Section: Discussionmentioning

confidence: 79%

Electroacupuncture facilitates the integration of a grafted TrkC‐modified mesenchymal stem cell‐derived neural network into transected spinal cord in rats via increasing neurotrophin‐3

Yang

Deng

et al. 2021

CNS Neurosci Ther

Self Cite

View full text Add to dashboard Cite

show abstract

“…EA can improve the motor function in rats with SCI by inhibiting signal transduction of semaphorin3A and upregulating the peripheral nerve networks (Hu et al, 2020). EA can also enhance the intrinsic growth ability of spinal neurons through activating the CGRP/αCaMKII/NT-3 pathway after SCI (Xu et al, 2021). The above-mentioned findings suggest that the efficacy of EA is closely related to the nervous system.…”

Section: Discussionmentioning

confidence: 94%

Effect of Schwann cell transplantation combined with electroacupuncture on axonal regeneration and remyelination in rats with spinal cord injury

Tan

Yang

Liu

et al. 2021

The Anatomical Record

View full text Add to dashboard Cite

show abstract

“…Except for ST36 and SP 6, there are many other acupoints which benefit spinal cord injury. Ding et al (2013) found Governor Vessel acupoints can also promote neuronal survival and axonal regeneration of injured spinal cord (Xu et al, 2021). Wong et al (2003) demonstrated that EA at SI3 and BL62 in conjunction with auricular acupoints produced enhanced recovery of bladder function in patients with SCI.…”

Section: Discussionmentioning

confidence: 97%

“…Ding et al. ( 2013 ) found Governor Vessel acupoints can also promote neuronal survival and axonal regeneration of injured spinal cord (Xu et al., 2021 ). Wong et al.…”

Section: Discussionmentioning

confidence: 99%

Effect of electroacupuncture on inhibition of inflammatory response and oxidative stress through activating ApoE and Nrf2 in a mouse model of spinal cord injury

et al. 2021

View full text Add to dashboard Cite

Introduction: Electroacupuncture protects neurons and myelinated axons after spinal cord injury by mitigating the inflammatory response and oxidative stress, but how it exerts these effects is unclear.Methods and results: Spinal cord injury was induced in C57BL/6 wild-type and apolipoprotein E (ApoE) knockout (ApoE -/-) mice, followed by electroacupuncture or ApoE mimetic peptide COG112 treatment. Mice with spinal cord injury suffered loss of myelinated axons and hindlimb motor function through the detections of Basso mouse scale, histology, and transmission electron microscopy; electroacupuncture partially reversed these effects in wild-type mice but not in ApoE -/mice. Combining exogenous ApoE administration with electroacupuncture significantly mitigated the effects of spinal cord injury in both mouse strains, and these effects were associated with up-regulation of anti-inflammatory cytokines and down-regulation of proinflammatory cytokines which were detected by quantitative reverse transcriptionpolymerase chain reaction. Combination treatment also reduced oxidative stress by up-regulating ApoE and Nrf2/HO-1 signaling pathway through the detections of immunofluorescence and western blot analysis. Conclusions:These results suggest that electroacupuncture protects neurons and myelinated axons following spinal cord injury through an ApoE-dependent mechanism.

show abstract

Governor Vessel Electro-Acupuncture Promotes the Intrinsic Growth Ability of Spinal Neurons through Activating Calcitonin Gene-Related Peptide/α-Calcium/Calmodulin-Dependent Protein Kinase/Neurotrophin-3 Pathway after Spinal Cord Injury

Cited by 21 publications

References 33 publications

Electroacupuncture facilitates the integration of a grafted TrkC‐modified mesenchymal stem cell‐derived neural network into transected spinal cord in rats via increasing neurotrophin‐3

Electroacupuncture facilitates the integration of a grafted TrkC‐modified mesenchymal stem cell‐derived neural network into transected spinal cord in rats via increasing neurotrophin‐3

Effect of Schwann cell transplantation combined with electroacupuncture on axonal regeneration and remyelination in rats with spinal cord injury

Effect of electroacupuncture on inhibition of inflammatory response and oxidative stress through activating ApoE and Nrf2 in a mouse model of spinal cord injury

Contact Info

Product

Resources

About