Gossypin Sıçanlarda Böbrek İskemi- Reperfüzyon Hasarına Karşı Korur

Abstract

Renal injury occurring as a result of renal ischemia-reperfusion may lead to renal failure or even death. The aim of this study is to investigate possible protective effects of Gossypin on tissue damage occurred due to ischemia-reperfusion in rat kidney tissue. A total of 48 male Wistar albino rats were used in the study. These rats were randomly divided into 6 groups equally (n = 8). The created groups were control (C), sham (S), ischemia-reperfusion (I/R), I/R + DMSO, I/R + 400 μg/kg gossypin and I/R + 4 mg/kg gossypin. In the rats of sham group, the right nephrectomy was performed. In the rats of other groups rather than sham, the left renal artery was clamped after performing the right nephrectomy. Gossypin was administered intraperitoneally before the reperfusion. 24 h reperfusion was applied to the left renal after 1 h of ischemia. TNF-α, IL-1β, IL-6 and IL-10 levels were measured with spectrophotometric methods in the kidney tissues after the procedures were completed. Apoptosis and inflammatory pathways were evaluated histopathologically using Caspase 3 and NF-κB antibodies. There was a statistically significant decrease in IL-1β and IL-6 levels of the gossypin groups compared to the I/R group (P<0.05). As the level of TNF-α was decreased in the gossypin administered groups compared to the I/R group although not statistically significant, the level of IL-10 was increased. In the present study, we aimed to show that gossypin in renal I/R model is effective on inflammatory process and apoptosis and that it can be used in routine treatment to decrease the damage in all reasons that may cause I/R. In addition, this study can shed light on the studies to be done in this field in the future. ÖzBöbrek iskemi reperfüzyonu sonucu meydana gelen renal hasar, böbrek yetmezliğine ve hatta ölüme neden olabilir. Çalışmanın amacı sıçan böbrek dokusunda iskemi-reperfüzyona bağlı oluşan doku hasarına karşı gossypinin olası koruyucu etkilerini araştırmaktır. Çalışmada toplam 48 adet Wistar albino cinsi erkek sıçan kullanıldı. Sıçanlar randomize ve eşit olmak üzere 6 gruba ayrıldı (n=8). Kontrol (C), sham (S), istemi-reperfüzyon (I/R), I/R + DMSO, I/R + 400 μg/kg gossypin ve I/R + 4 mg/kg gossypin grupları oluşturuldu. Sham grubunda arka bölge açılarak sağ nefrektomi yapıldı. Gossypin reperfüzyondan önce intraperitoneal olarak uygulandı. Sol renal artere 1 saat iskemi sonunda 24 saat reperfüzyon uygulandı. Prosedürler tamamlandıktan sonra böbrek dokularında spektrofotometrik metodlarla TNF-α, IL-1β, IL-6 and IL-10 seviyeleri ölçüldü. Apoptozis ve inflamatuvar yolaklar kazpaz-3 ve NF-κB antikorları kullanılarak değerlendirildi. Gossypin grupları I/R grupları ile kıyaslanınca IL-1β and IL-6 seviyelerinde istatistiksel olarak önemli derecede azalma tespit edildi (P<0.05). I/R grubu ile kıyaslandığında gossypin uygulanan gruplarda istatistiksel olarak anlamlı olmasa da TNF-α miktarlarına azalma tespit edildi. Mevcut çalışmada gossypinin I/R modelindeki inflamasyon aşaması ve apoptosis üzerine etkisini ve I/R neden olan tüm durum...

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“…Gossypin is a bioflavonoid found in the Hibiscus vitifolius plant, which belongs to the Malvaceae family. It is a flavone with a chemical structure that includes 3,5,8,3,4‐pentahydroxy‐7‐O‐glucosyl and an 8‐glucoside group 22–24 . Bioflavonoids are plant‐based compounds that are known for their antioxidant properties and potential health benefits 23,25 .…”

“…It is a flavone with a chemical structure that includes 3,5,8,3,4‐pentahydroxy‐7‐O‐glucosyl and an 8‐glucoside group 22–24 . Bioflavonoids are plant‐based compounds that are known for their antioxidant properties and potential health benefits 23,25 . Gossypin also exhibited the protective effect against the pancreatic β‐cells from the glucotoxicity in the diabetic rats 26 .…”

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