Gonadotropin-releasing hormone analogue multiple injection potentially accelerated testicular maturation of male yellow catfish (Pelteobagrus fluvidraco, Richardson) in captivity

Abstract

The potential for accelerating testicular development and ¢nal maturation of adult male yellow cat¢sh (Pelteobagrus £uvidraco, Richardson) in captivity was evaluated by using gonadotropin-releasing hormone analogue (GnRHa) multiple injection, and its possible regulatory mechanism was explored by measurements of luteinizing hormone (LH) and steroid levels. The results showed that GnRHa multiple injection increased gonad-somatic index (GSI), the sperm count and the milt-testis index (MTI) and promoted the larvae production by elevated rates of fertilization and hatching, and lowered the rate of deformed larvae. Meanwhile GnRHa treatment decreased the percentages of spermatogonia and spermatocytes, increased the relative sperm density (RSD) and percentages of spermatids and spermatozoa (STZ), suggesting GnRHa treatment could accelerate meiotic division and enhance the formation of spermatids andSTZ. Moreover, pituitary and serum LH, as well as serum T levels were increased after GnRHa multiple injection. Taken together, the present study demonstrated GnRHa multiple injection successfully accelerated the testicular development and maturation of male yellow cat¢sh, which was mediated by the increase in serum LH and T levels that further mediated biological and histological changes of testis. Therefore, GnRHa multiple injection could be a valuable method to induce precocious testicular maturation of adult yellow cat¢sh with gonadal developmental arrest in captivity.

Recent Comparative Studies on the Performance and Survival Rate of African Catfish (Clarias gariepinus) Larval Produced under Natural and Synthetics Hormones: A Review

“…Gonadotropin Releasing Hormone analogue (GnRHa) is now the best available biotechnological tool for the induced breeding of fish. These includes the following which have been used to induce breeding successfully, Ovaprim, Ovatide, Ovaryprim, Ovopel, Ovupin-L, Ovulin, Dagin and Aquaspawn [12,13,[26][27][28]. Among Gonadotropin Releasing Hormone analogue (GnRHa), Ovaprim and Ovatide containing salmon gonadotropin releasing hormone analogue and domperidone (SGnRHa + Domperidone) which are usually used for spawning induction in catfishes to get quality seed [29].…”

“…These reproductive processes are controlled through the brain-pituitary gonadal axis. The brain is stimulated by environmental cues (water rise, temperature, feeding, rainfall, and photoperiod) to release gonadotropin releasing hormones [10]. Then, ovulation and spermiation are affected as a result of the sex steroids that have been produced [11].…”

Sperm Quality and reproductive performance of male Clarias Gariepinus induced with synthetic hormones (Ovatide and Ovaprim)

“…Ovulation and spermiation are effected as a result of the sex steroids that have been produced. Administration of gonadotropin releasing hormone analogue has been reported to increase levels of plasma sex steroids in fish (Zhuo et al, 2011).…”

INDUCTION OF GONADAL MATURATION OF POND CULTURED MALE TIGER SHRIMP, Penaeus monodon WITH DIFFERENT DOSAGES OF GONADOTROPIN RELEASING HORMONE ANALOGUE AGAINST EYE STALK ABLATION

“…In addition, clear signs of spermiation and lobules filled with a large number of spermatozoa also demonstrated in testes from sGnRHa treated fish by identifying the testes morphological differences compared to HCG-injected groups. A stimulatory effect of this hormone on germ cell pro- liferation and spermatozoid differentiation has been also reported in marine fish the golden rabbitfish (Komatsu et al, 2006) and freshwater species of Senegalese sole (Guzman et al, 2011) and yellow catfish (Zhuo et al, 2012).…”