Gonadotrophin administration can benefit ovarian tissue grafted to the body wall: implications for human ovarian grafting

Imthurn, Bruno; Cox, Shae-Lee; Jenkin, Graham; Trounson, Alan; Shaw, Jillian M

doi:10.1016/s0303-7207(00)00218-5

Cited by 66 publications

(46 citation statements)

References 16 publications

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“…Considering the fact that early initiation of angiogenesis is a crucial factor for the maintenance of transplanted ovarian tissues, angiogenic factors need to be present in effective amounts at transplanted position, for the critical time immediately after transplantation in order to induce neovascularization and therefore prevent ischaemic hypoxia [18].…”

Section: Introductionmentioning

confidence: 99%

VEGF and bFGF increase survival of xenografted human ovarian tissue in an experimental rabbit model

Wang

Ying

OuYang

et al. 2013

J Assist Reprod Genet

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Section: Introductionmentioning

confidence: 99%

VEGF and bFGF increase survival of xenografted human ovarian tissue in an experimental rabbit model

Wang

Ying

OuYang

et al. 2013

J Assist Reprod Genet

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“…Furthermore, it causes an additional loss of follicles if administered during the critical neovascularization period after the transplantation [42], and significant loss of primordial follicles after prolonged gonadotropin stimulation in xenografts [43]. However, Imthurn et al [26] showed an increase in the total number of growing follicles in the graft with administration of recombinant human FSH and LH.…”

Section: Discussionmentioning

confidence: 99%

“…So the survival of ovarian implants was improved under conditions that support vascular maintenance. As well, the extensive capillary bed of KC naturally favours rapid graft revascularization [26].…”

Section: Discussionmentioning

confidence: 99%

“…The time needed to achieve an adequate perfusion of the transplanted tissue with new blood vessels is critical for the follicular survival and the functional longevity of the graft [25]. More importantly, vascular anastomosis is likely to be influenced by angiogenic growth factors [26]. The mRNA for vascular endothelial growth factor (VEGF) and transforming growth factor beta (TGF-b), a very potent angiogenic factor, are normally expressed in the ovary [27] and are rapidly upregulated by hypoxia as well as by gonadotropins [28].…”

Section: Introductionmentioning

confidence: 99%

“…However, the effect of gonadotrophins either before or after graft on further follicular development or fertility restoration on human and animal ovarian tissue grafting to mice is still controversial [25]. The administration of gonadotrophins to recipient mice after transplantation under kidney capsule (KC) [30] or abdominal wall [26], or in ovarian pedicle [15] showed no difference in follicular survival. In contrast, several reports showed an increase in the total number of growing follicles and can initiate follicular growth in the graft with administration of gonadotrophins [31][32][33].…”

Section: Introductionmentioning

confidence: 99%

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Effect of graft site and gonadotrophin treatment on follicular development of canine ovarian grafts transplanted to NOD‐SCID mice

Abdel-Ghani

Abe

Asano

et al. 2011

Reprod Medicine & Biology

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Purpose To investigate the effect of graft site and gonadotrophins administration on the number and survival rate of follicles of canine ovarian grafts transplanted to NOD-severe combined immune deficiency (SCID) mice. Methods Fresh ovarian cortex slices obtained from immature bitches were grafted subcutaneously (SC), under kidney capsule (KC) or into ovarian bursa (OB) in NOD-SCID mice. Two months after surgery, the mice allocated into non-treated and treated gonadotrophins groups that injected with porcine follicle stimulating hormone during 7 days and human chorionic gonadotrophin 48 h later. Ovarian grafts were collected after 10 h of last injection and processed for histology. Results The number of transitional and preantral follicles under KC and into OB was significantly higher in gonadotrophins-treated mice than those who received saline. Furthermore, the survival rates of primary, transitional and preantral follicles under KC and into OB grafts were significantly higher than those placed SC in the treated gonadotrophins group, and in the non-treated gonadotrophins group; the proportion of primary and preantral follicle survival was significantly higher under KC and into OB than SC grafts. Conclusions In canine ovarian xenografting, administration of gonadotrophin could be effective for improvement of survival of transplanted ovary. Furthermore, the grafting into OB appeared to be better than grafting under KC, which in turn is better than SC.

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Angiogenesis of the Frozen‐Thawed Human Fetal Ovarian Tissue at the Early Stage after Xenotransplantation and the Positive Effect of Salviae miltiorrhizae

Ya-ning

Tong

et al. 2010

The Anatomical Record

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Cryopreserving ovarian tissue followed by transplantation has been suggested to preserve fertility for young cancer survivors. However, ischemia in the early stage after transplantation causes massive follicle loss. The aim was to investigate the histological and ultrastructural characteristics of the frozen-thawed human fetal ovarian tissue after xenotransplantation and the effects of Salviae miltiorrhizae (SM) on the angiogenesis. The human fetal ovarian tissues were frozen-thawed, xenografted into the immunodeficient nu/nu mice, and then collected 2, 7, and 28 days after transplantation. SM was administered. Compared with that of the frozen-thawed ovarian tissue, the total follicle number of the grafts was greatly reduced. Nearly half of the primordial follicles were damaged at different levels on day 2. Moreover, edema was prevalent in the stroma during the first week after the graft, especially on day 2. The microvessel density of the grafts was increased on day 2, reached a peak on day 7, and then declined on day 28. Both healthy primordial follicle proportion and the total healthy primordial follicles pool in the SM group were significantly higher than those of the control group (P ¼ 0.003 and P ¼ 0.001). We found a statistically significant difference of microvessel density between the two groups on day 2 (P < 0.001). In the frozenthawed fetal ovarian grafts, angiogenesis has been begun on day 2, and the first week is the critical time for the grafts to regain their function, in which SM can facilitate graft vascularization and improve the preservation of primordial follicles. Anat Rec, 293:2154Rec, 293: -2162

show abstract

Gonadotrophin administration can benefit ovarian tissue grafted to the body wall: implications for human ovarian grafting

Cited by 66 publications

References 16 publications

VEGF and bFGF increase survival of xenografted human ovarian tissue in an experimental rabbit model

VEGF and bFGF increase survival of xenografted human ovarian tissue in an experimental rabbit model

Effect of graft site and gonadotrophin treatment on follicular development of canine ovarian grafts transplanted to NOD‐SCID mice

Angiogenesis of the Frozen‐Thawed Human Fetal Ovarian Tissue at the Early Stage after Xenotransplantation and the Positive Effect of Salviae miltiorrhizae

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