Golgi-Cox Staining Step by Step

Zaqout, Sami; Kaindl, Angela M.

doi:10.3389/fnana.2016.00038

Cited by 159 publications

(117 citation statements)

References 13 publications

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“…Step by Step" (Zaqout & Kaindl, 2016). In the impregnation step, silver nitrate is replaced with mercury chloride, and similar to the other methods, potassium dichromate and potassium chromate are used in this step.…”

Section: Specific Points Regarding Each Methods Golgi-cox Methodsmentioning

confidence: 99%

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Comprehensive Review of Golgi Staining Methods for Nervous Tissue

Kang

Kim

Moon

et al. 2017

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Golgi staining has been modified and developed since Camillo Golgi introduced the black reaction in 1873. This study focuses on the commonly used Golgi staining methods and presents comprehensive data regarding three Golgi staining methods along with their strong and weak points. The Golgi-Cox method uses mercuric chloride for brain tissue impregnation and is a reliable technique for analyzing the complete dendritic tree of cortical neurons. However, specimens tend to shrink during the staining steps. Recent combination of the Golgi-Cox method and immunofluorescence provides additional options for neuroscientists. Rapid Golgi staining requires osmium tetroxide for the postfixation process. It homogenously stains whole structures of neurons and provides their detailed anatomical morphology. This staining is influenced by the age of the specimen, temperature of the laboratory, and duration of each procedure. The Golgi-Kopsch method uses formaldehyde and glutaraldehyde instead of osmium tetroxide and can be used regardless of the age of the specimen and the duration after fixation. This method is suitable for research using human brain fixed for a long time or for specimens obtained from old-aged animals. Selecting a Golgi staining protocol that is appropriate for the specimen type and research purpose is important to achieve best results.

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Section: Specific Points Regarding Each Methods Golgi-cox Methodsmentioning

confidence: 99%

“…In the color development process, the specimen is dehydrated and developed. Dehydration using ethanol and cleaning with xylol are repeated many times, resulting in further quality improvement (Zaqout & Kaindl, 2016). The specimens are then mounted and observed.…”

Section: Specific Points Regarding Each Methods Golgi-cox Methodsmentioning

confidence: 99%

Comprehensive Review of Golgi Staining Methods for Nervous Tissue

Kang

Kim

Moon

et al. 2017

View full text Add to dashboard Cite

show abstract

“…O protocolo adaptado para Golgi-Cox 6,19 apresentado neste estudo também se mostrou eficiente na identificação dos neurônios piramidais, e apesar da técnica também promover a coloração de células gliais e vasos sanguíneos, foi possível fazer a identificação segura dos neurônios devido à impregnação dos prolongamentos neuronais e das espinhas dendríticas (Figura 3).…”

Section: Resultado E Discussãounclassified

O Desafio Para Compreender a Morfologia Cerebral: Da Visualização À Quantificação

Melo

Oliveira

et al. 2019

SaudPesq

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O objetivo deste trabalho é estudar a morfologia neuronal a partir de modelos animais, fornecer informações biológicas difíceis de serem obtidas em humanos, permitindo estudar condições neuropsiquiátricas como doença de Alzheimer, ansiedade, dentre outras. O presente trabalho descreveu metodologia de estudo para cérebro de roedores, duas técnicas neuroanatômicas, Klüver-Barrera e Golgi-Cox, e seus respectivos processos de quantificação. A técnica de Klüver-Barrera permitiu visualização da substância branca e cinzenta com destaque na bainha de mielina. A técnica de Golgi-Cox, adaptada para realidade de nosso laboratório, mostrou-se eficiente para visualização de neurônios e seus prolongamentos, como dendritos e espinhas dendríticas, permitindo assim a quantificação. A partir de imagens obtidas de microscópio descreveu-se os diferentes passos para quantificação, a determinação de volume de estruturas internas cerebrais (corpo caloso e camada celular do hipocampo) assim como a quantificação das espinhas dendríticas em neurônios piramidais. Os métodos descritos e detalhados poderão ser utilizados em vários campos da neurociência.

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“…in the layers of the hippocampus, the trees can be highly anisotropic to support inter-layer connectivity. 25,26…”

Section: Dendritesmentioning

confidence: 99%

Imaging brain microstructure with diffusion MRI: practicality and applications

et al. 2017

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This article gives an overview of microstructure imaging of the brain with diffusion MRI and reviews the state of the art. The microstructure‐imaging paradigm aims to estimate and map microscopic properties of tissue using a model that links these properties to the voxel scale MR signal. Imaging techniques of this type are just starting to make the transition from the technical research domain to wide application in biomedical studies. We focus here on the practicalities of both implementing such techniques and using them in applications. Specifically, the article summarizes the relevant aspects of brain microanatomy and the range of diffusion‐weighted MR measurements that provide sensitivity to them. It then reviews the evolution of mathematical and computational models that relate the diffusion MR signal to brain tissue microstructure, as well as the expanding areas of application. Next we focus on practicalities of designing a working microstructure imaging technique: model selection, experiment design, parameter estimation, validation, and the pipeline of development of this class of technique. The article concludes with some future perspectives on opportunities in this topic and expectations on how the field will evolve in the short‐to‐medium term.

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Golgi-Cox Staining Step by Step

Cited by 159 publications

References 13 publications

Comprehensive Review of Golgi Staining Methods for Nervous Tissue

Comprehensive Review of Golgi Staining Methods for Nervous Tissue

O Desafio Para Compreender a Morfologia Cerebral: Da Visualização À Quantificação

Imaging brain microstructure with diffusion MRI: practicality and applications

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