GoldMag nanocomposite-functionalized graphene sensing platform for one-step electrochemical immunoassay of alpha-fetoprotein

Zhang, Bing; Tang, Dianping; Liu, Bingqian; Chen, Huafeng; Cui, Yuling; Chen, Guonan

doi:10.1016/j.bios.2011.07.016

Cited by 49 publications

(21 citation statements)

References 37 publications

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“…In recent years it has been proven that electrode material and structure play critical roles to achieve simple, sensitive, rapid, and stable detection in bioanalysis using electrochemical or piezoelectrical methods [1][2][3]. Nanomaterials and three-dimensional (3D) nanostructures have been introduced into electrode structures to improve the performance of electrical measurements [4][5][6].…”

Section: Introductionmentioning

confidence: 99%

A nanowell-based QCM aptasensor for rapid and sensitive detection of avian influenza virus

Wang

Callaway

et al. 2017

Sensors and Actuators B: Chemical

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In this study, a cost-effective nanowell structure was fabricated and utilized for the development of a nanowell-based quartz crystal microbalance (QCM) aptasensor for rapid, sensitive, and label-free detection of H5N1 avian influenza virus (AIV). A nanoporous gold film with a thickness of 120 nm and a pore size of ~20 nm was prepared using a metallic corrosion method. Then, the nanoporous gold film was immobilized onto a gold electrode surface using a self-assembled monolayer to form a nanowell-based electrode. A specific H5N1 AIV ssDNA aptamer with a NH 2 conjugated 5'-terminal was used in the fabrication of the QCM aptasensor through covalent bonding. The stepwise assembly of the aptasensor was characterized by means of QCM. The result showed that the binding of target AIV H5N1 onto the immobilized aptamers decreased the sensor's resonant frequency, and the frequency change correlated to the virus titer. We demonstrated that the developed nanowell-based QCM aptasensor could dramatically reduce detection time down to 10 min using a label-free assay. The detection range of 2-4 to 2 4 hemagglutination units (HAUs) /50 μl was obtained with a detection limit of 2-4 HAU/50 μl for AIV H5N1. The binding of target H5N1 virus onto the nanowell-based electrode surface was further confirmed by scanning electron microscopy (SEM). No interference was observed from non-target AIV subtypes of H1N1, H2N2, H7N2 and H5N3. The aptasensor using H5N1 aptamer was validated for the detection of AIV H5N1 in chicken tracheal swab samples. The developed aptasensor could be adopted for detection of other viruses.

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Section: Introductionmentioning

confidence: 99%

A nanowell-based QCM aptasensor for rapid and sensitive detection of avian influenza virus

Wang

Callaway

et al. 2017

Sensors and Actuators B: Chemical

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“…Heterogeneous immunoassays are easier to design and construct, but they require a separation of the Ag and the Ab from the samples and their immobilization at a solid surface. In contrast, the homogeneous immunoassays usually involve the immobilization of the biomolecules on nano-/microbeads, thus allowing the integration of multiple liquid handling processes [23,24]. The homogeneous immunoassays coupled with microfluidic devices are relatively inexpensive and efficient alternatives and can be used for the detection of complex samples, such as urine or blood, without any sample pre-treatment or large volume consumption.…”

Section: Label-free Versus Labelled Immunosensorsmentioning

confidence: 99%

“…The distinction has an entirely different meaning to the terms direct and indirect in the immunoassay fields, which are both tracer-related. They are distinguished by whether Ab binding is directly detected, for example, after an enzymelabelled Ab is bound to an immobilized coating conjugate or an enzyme tracer to an immobilized Ab, or whether the detection only takes place after a secondary binding reaction, for example, if an enzyme-labelled secondary Ab is used to label the first Ab bound to an immobilized coating conjugate [21,22,23].…”

Section: Label-free Versus Labelled Immunosensorsmentioning

confidence: 99%

Immunosensors

Cristea¹,

Florea²,

Tertiş³

et al. 2015

Biosensors - Micro and Nanoscale Applications

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Immunosensors are solid-state devices in which the immunochemical reaction is coupled to a transducer. They form one of the most important classes of affinity biosensors based on the specific recognition of antigens by antibodies to form a stable complex, in a similar way to immunoassay. Depending on the type of transducer there are four types of immunosensor: electrochemical, optical, microgravimetric and thermometric. The most commonly used bioelements for the development of electrochemical immunosensors are antibodies (Ab), followed by aptamers (Apt) and, in the last five years, microRNA (miRNA). In order to perform an early diagnosis, a method that is able to measure peptides and proteins directly in a sample, without any sample pre-treatment or any separation, is preferred. This direct detection can be performed with methods making use of the specific interaction of proteins with Ab, Apt and miRNA. The recent developments made in the immunosensor field, regarding the incorporation of nanomaterials for increased sensitivity, multiplexing or microfluidic-based devices, may have potential for promising use in industry and clinical analysis. Some examples of assays for several commercially available biomarkers will be presented. The main application fields, beside biomedical analysis, are drug abuse control, food analysis and environmental analysis.

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“…So far, many techniques have been developed for the detection of AFP, such as enzyme-linked immunoassay (ELISA) (Liew et al, 2007), electrochemical luminescence (ECL) (Qian et al, 2010;Yang et al, 2009), electrochemical method (Dai et al, 2011;Tang et al, 2010;Zhang et al, 2011), mass spectrometry (Stevens et al, 2008), quartz crystal microbalance (QCM) (Chou et al, 2002) and surface plasmon resonance (SPR) (Teramura and Iwata, 2007) immunity determination.…”

Section: Introductionmentioning

confidence: 99%

A sensitive photoelectrochemical biosensor for AFP detection based on ZnO inverse opal electrodes with signal amplification of CdS-QDs

Jiang

Xia

et al. 2015

Biosensors and Bioelectronics

117

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GoldMag nanocomposite-functionalized graphene sensing platform for one-step electrochemical immunoassay of alpha-fetoprotein

Cited by 49 publications

References 37 publications

A nanowell-based QCM aptasensor for rapid and sensitive detection of avian influenza virus

A nanowell-based QCM aptasensor for rapid and sensitive detection of avian influenza virus

Immunosensors

A sensitive photoelectrochemical biosensor for AFP detection based on ZnO inverse opal electrodes with signal amplification of CdS-QDs

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