Gold surface patterned with cyclodextrin-based molecular nanopores for electrochemical assay of transglutaminase activity

Nanochannel-based electrochemical assay for transglutaminase activity

“…In this context, we recently described a novel voltammetric strategy to quantify TGase activity by using cyclodextrin-patterned Au surfaces. 6 However, this approach implied a time consuming protocol for the synthesis of the oligosaccharide derivative and the sequential assembly of the sensing surface.…”

“…It is important to emphasize that the proposed assay improves considerably the analytical performance with respect to that described previously using a gold surface patterned with cyclodextrin-based molecular nanopores in terms of a wider linear range at low enzyme concentrations (0.4-185 mU mL À1 vs. 1.9-37 mU mL À1 ) and a much higher sensitivity (6.3 mA mL U À1 s À1 vs. 1.4 Â 10 À9 mA mL U À1 s À1 ). 6 The electrochemical assay also showed the potentiality to be used for the determination of Pb 2+ in water due to the irreversible inhibition of TGases by this ion 9 as a consequence of the presence of an active thiol group at the active site of these enzymes. 10 The assay was performed by comparing the time-dependent voltammetric behavior of the nanostructured electrode in the presence of 15 mM CBZ and 100 mU mL À1 TGase with and without addition of Pb(NO 3 ) 2 at different concentrations.…”