Nanochannel-based electrochemical assay for transglutaminase activity

Fernández, Iñigo; Sánchez, Alfredo; Díez, Paula; Martı́nez-Ruiz, Paloma; Pierro, Próspero Di; Porta, Raffaele; Villalonga, Reynaldo; Pingarrón, José M.

doi:10.1039/c4cc05083e

Cited by 27 publications

(17 citation statements)

References 16 publications

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“…The issue here is to develop suitable hybrid materials for stabilization of the protein and promoting enzymatic activity and, possibly, efficient electron transfers. The future could be a further miniaturization and integration in lab‐on‐chip devices, as illustrated by the development of nanofluidic in mesoporous channels 236, nanomachine or nanomotors based on mesoporous silica particles 237, 238 and nanochannel‐based electrochemical assays 239.…”

Section: Discussionmentioning

confidence: 99%

Mesoporous Materials‐Based Electrochemical Enzymatic Biosensors

et al. 2015

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This review (239 references) is dealing with the use of mesoporous materials in the field of electrochemical enzymatic biosensors. After a brief discussion on the interest of mesoporous materials for electrochemical biosensing, a presentation of the various types of inorganic and organic‐inorganic hybrid mesostructures used to date in the elaboration of enzymatic bioelectrodes is given and the main bioelectrode configurations are described. The various biosensor applications are then summarized on the basis of a comprehensive table and some representative illustrations. The main detection schemes developed in the field are based on amperometry and mediated electrocatalysis, as well as some on spectroelectrochemistry.

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Section: Discussionmentioning

confidence: 99%

Mesoporous Materials‐Based Electrochemical Enzymatic Biosensors

et al. 2015

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“…For example, Fernández et al have functionalized the channel walls of VMSFs to construct a novel turn-off electrochemical assay for evaluating transglutaminase (TGase) activity. 89 The detection rested on the molecular transport of electroactive probes, namely Fe(CN) 6 3− , through amine-functionalized silica mesochannels controlled by TGases. TGases can catalyze the linking of N-benzyloxycarbonyl-L-glutaminylglycine with tethered amino groups on the channel walls, which physically blocked the mesochannels and hindered the diffusion of Fe(CN) 6 3− to the underlying electrode, thus resulting in an obvious current decrease associated with the reduction of Fe(CN) 6 3− .…”

Section: Size Selective Effectmentioning

confidence: 99%

Vertically ordered silica mesochannel films: electrochemistry and analytical applications

Yan

Lin

2016

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Mesoporous silica films consisting of highly ordered and vertically aligned nanochannels (abbreviated as VMSFs) have received considerable attention because of their high surface area, long-range order, thermal and mechanical stability, controllable pore size and more importantly good molecular accessibility for rapid mass transport. These characteristics are ideal for electroanalytical chemistry and separation science. In this review, we firstly present briefly the strategies for the synthesis of VMSFs on the electrode surface, mainly the electrochemically assisted self-assembly and Stöber-solution growth approaches, as well as the surface modification of channel walls with diverse terminal groups for various functionalities. In the next section, recent progress on the applications of VMSFs in electroanalytical chemistry and sensing is summarized, in terms of the spatial confinement and permselective effects (size, charge and lipophilicity selectivity of the mesochannels). We then present the preparation and application of perforated free-standing VMSFs for fast and precise molecular sieving/separation. The review ends with an outlook and perspective on the future applications of VMSFs.

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“…Regarding MSFs, to date, they have always been used as a modifier of conventional gold electrodes in catalytic or affinity biosensing methods to target transglutaminase activity (TGase) [ 196 ] Cu 2+ /ascorbic acid (AA) [ 195 ], E. coli 16S rRNA [ 175 ], prostate-specific antigen (PSA) [ 176 ], and streptomycin (STR) [ 177 ]. Villalonga’s group proposed an electrochemical assay to quantify TGase activity via the enzyme-controlled diffusion of Fe(CN) 6 3−/4− through amino-functionalized nanochannels of a MSF-AuE [ 196 ]. The nanochannels were selectively gated by the catalytic action of TGase in the presence of the glutamine-donor substrate N-benzyloxycarbonyl-L -glutaminylglycine (CBZ).…”

Section: Multifunctional Silica Nanomaterialsmentioning

confidence: 99%

Multimodal/Multifunctional Nanomaterials in (Bio)electrochemistry: Now and in the Coming Decade

et al. 2020

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Multifunctional nanomaterials, defined as those able to achieve a combined effect or more than one function through their multiple functionalization or combination with other materials, are gaining increasing attention in the last years in many relevant fields, including cargo targeted delivery, tissue engineering, in vitro and/or in vivo diseases imaging and therapy, as well as in the development of electrochemical (bio)sensors and (bio)sensing strategies with improved performance. This review article aims to provide an updated overview of the important advances and future opportunities exhibited by electrochemical biosensing in connection to multifunctional nanomaterials. Accordingly, representative aspects of recent approaches involving metal, carbon, and silica-based multifunctional nanomaterials are selected and critically discussed, as they are the most widely used multifunctional nanomaterials imparting unique capabilities in (bio)electroanalysis. A brief overview of the main remaining challenges and future perspectives in the field is also provided.

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Nanochannel-based electrochemical assay for transglutaminase activity

Cited by 27 publications

References 16 publications

Mesoporous Materials‐Based Electrochemical Enzymatic Biosensors

Mesoporous Materials‐Based Electrochemical Enzymatic Biosensors

Vertically ordered silica mesochannel films: electrochemistry and analytical applications

Multimodal/Multifunctional Nanomaterials in (Bio)electrochemistry: Now and in the Coming Decade

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