Gold‑platinum bimetallic nanoparticles coated 3-(aminopropyl)triethoxysilane (APTES) based electrochemical immunosensor for vitamin D estimation

Kaur, Amandeep; Kapoor, Shivali; Bharti, Anu; Rana, Shilpa; Chaudhary, Ganga Ram; Prabhakar, Nirmal

doi:10.1016/j.jelechem.2020.114400

Cited by 34 publications

(17 citation statements)

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“…The result can be achieved rapidly and conveniently, allowing for quick decision-making, thus minimizing the risk of human-to-human transmission. Few reports are available in the literature for the development of VD biosensors including the previous work done in our lab for VD detection [ 4 , 11 – 16 ]. However, the biosensor developed for 25VD 3 is very limited as shown in Table 1 .…”

Section: Introductionmentioning

confidence: 99%

“…However, the use of the Au electrode is costly, and also the SPR technique requires expertise to handle the instrument [ 15 ]. One recent report for 25VD 3 detection by Kaur et al utilized gold-platinum bimetallic nanoparticles coated 3-(aminopropyl)triethoxysilane (APTES) for immunosensor (Ab-25(OH)D 3 /Glut/Au-Pt/APTES) to detect 25VD 3 [ 16 ]. The main shortcomings of the previous reports are the lack of validation study through a conventional technique of ELISA and also the real samples were not analyzed to test the efficiency of the electrodes.…”

Section: Introductionmentioning

confidence: 99%

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Carbon cloth-based immunosensor for detection of 25-hydroxy vitamin D3

2021

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Vitamin D (VD) deficiency is a global health concern due to its serious health impacts, and at present, the monitoring of VD status is expensive. Here, a novel immunosensor for sensitive and label-free detection of 25-hydroxy vitamin D 3 (25VD 3 ) is reported. Nanostructured cerium(IV) oxide (nCeO 2 ) was anchored onto carbon cloth (CC) via electrophoretic deposition to fabricate a nanoplatform (nCeO 2 /CC). Subsequently, bioactive molecules (anti-25VD 3 and BSA) were introduced to fabricate the nanobioplatform BSA/anti-25VD 3 /nCeO 2 /CC as an immunosensor. The analytical performance of the developed immunosensor was studied towards 25VD 3 detection. The immunosensor provides a broad linear range of 1-200 ng mL -1 , high sensitivity of 2.08 μA ng −1 mL cm −2 , a detection limit of 4.63 ng mL −1 , and a response time of 15 min, which is better than that of previous reports. The biosensor exhibited high selectivity, good reproducibility, and excellent stability for about 45 days. The potential application of the proposed immunosensor was observed for real serum samples towards 25VD 3 detection that demonstrated a high correlation with the conventional enzyme-linked immunosorbent assay. Graphical abstract Supplementary Information The online version contains supplementary material available at 10.1007/s00604-021-04751-y.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Carbon cloth-based immunosensor for detection of 25-hydroxy vitamin D3

2021

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“…Among the electrochemical approaches, these include flexible wearable and point-of-care enzymatic amperometric sensors for dynamic changes of VC in sweat, tears, saliva, urine, or blood ( Sempionatto et al, 2020 ; Zhao et al, 2021 ). Similarly, electrochemical VD immunosensors were recently described based on electrodes modified with different nanomaterials ( Carlucci et al, 2013 ; Chauhan et al, 2018 ; Chauhan et al, 2019 ; Kaur et al, 2020 ; Sarkar et al, 2018 ), with only one study involving an assay of body fluid (serum) ( Kaur et al, 2020 ). The sophistication of transduction has limited the use of optical approaches for direct VD detection in biological fluids ( Prante et al, 2019 ; Di Meo et al, 2020 ), with only few examples described smartphone-assisted optical approaches applied solely in diluted ( Walter et al, 2020 ) or filtered serum samples ( Lee et al, 2014 ).…”

Section: Introductionmentioning

confidence: 99%

Decentralized vitamin C & D dual biosensor chip: Toward personalized immune system support

Montiel

Sempionatto

Vargas

et al. 2021

Biosensors and Bioelectronics

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Combating the ongoing COVID-19 pandemic has put the spotlight on nutritional support of the immune system through consumption of vitamins C and D. Accordingly, there are urgent demands for an effective on-the-spot multi-vitamin self-testing platform that monitors the levels of these immune-supporting micronutrients for guiding precision nutrition recommendations. Herein, we present a compact bioelectronic dual sensor chip aimed at frequent on-the-spot simultaneous monitoring of the salivary vitamin C and D dynamics. The new bioelectronic chip combines a new electrocatalytic vitamin C amperometric assay along with competitive vitamin D immunoassay on neighboring electrodes, to perform selective and cross-talk free detection of both vitamins in a 10-μL saliva sample within 25 min. The distinct vitamin C or D temporal profiles obtained for different individuals after vitamin supplementation indicate the potential of the new bioelectronic chip strategy for enhancing personalized nutrition towards guiding dietary interventions to meet individual nutrition needs and promote immune system health.

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“…In our previous attempt, a Au-Pt NPs supported on 3-(aminopropyl)triethoxysilane-modified FTO (fluoride tin oxide) electrode as a biosensing platform for the electrochemical detection of 25(OH)D 3 was designed. The LOD was 0.49 pg mL −1 with stability of 10 days [15]. Herein, we have designed a strategy to further improve the analytical performance of the biosensor for detection of 25(OH)D 3 .…”

Section: Introductionmentioning

confidence: 99%

Voltammetric detection of vitamin D employing Au-MoS2 hybrid as immunosensing platform

et al. 2021

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A voltammetric immunosensor based on molybdenum sulphide (MoS 2 ) and gold nanoparticles (Au NPs) for the determination of 25-hydroxy vitamin D 3 (25(OH)D 3 ) is reported. Anti-vit D (Ab-25(OH)D 3 ) was immobilized onto the cysteamine-modified MoS 2 and Au NPs which were deposited onto a fluoride tin oxide (FTO) electrode (Ab/Cys/Au/MoS 2 /FTO). The MoS 2 sheets were prepared by hydrothermal method followed by an in situ growth of Au film onto the MoS 2 /FTO surface. Self-assembled monolayer (SAM) of cysteamine was synthesized onto the Au/MoS 2 /FTO which acts as a linker to covalently bind Ab-25(OH)D 3 . The Ab-25(OH)D 3 -immobilized Cys/Au/MoS 2 /FTO was used to detect 25(OH)D 3 using differential pulse voltammetry. The electrochemical system provided an anodic peak current at a potential of +0.21 V vs. Ag/AgCl (satd. KCl) of ferricyanide/ferrocyanide redox couple. The detection principle relies on the inhibition of electron transfer at the electrode surface owing to the hindrance caused by the formation of immune complex between Ab-25(OH)D 3 and 25(OH)D 3 . The immunosensor shows linear response from 1 pg mL −1 to 100 ng mL −1 25(OH)D 3 and a sensitivity of 189 μA [log (pg mL −1 )] −1 cm −2 along with a low limit of detection (LOD) of 0.38 pg mL −1 . The immunosensor is highly selective towards 25(OH)D 3 and presented a long shelf life of 28 days. Also, the immunosensor exhibits satisfactory performance towards spiked human serum samples with recovery between 95.1 and 102% (RSD 1.15-3.22%).

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Gold‑platinum bimetallic nanoparticles coated 3-(aminopropyl)triethoxysilane (APTES) based electrochemical immunosensor for vitamin D estimation

Cited by 34 publications

References 50 publications

Carbon cloth-based immunosensor for detection of 25-hydroxy vitamin D3

Carbon cloth-based immunosensor for detection of 25-hydroxy vitamin D3

Decentralized vitamin C & D dual biosensor chip: Toward personalized immune system support

Voltammetric detection of vitamin D employing Au-MoS2 hybrid as immunosensing platform

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