Carbon cloth-based immunosensor for detection of 25-hydroxy vitamin D3

Abstract

Vitamin D (VD) deficiency is a global health concern due to its serious health impacts, and at present, the monitoring of VD status is expensive. Here, a novel immunosensor for sensitive and label-free detection of 25-hydroxy vitamin D 3 (25VD 3 ) is reported. Nanostructured cerium(IV) oxide (nCeO 2 ) was anchored onto carbon cloth (CC) via electrophoretic deposition to fabricate a nanoplatform (nCeO 2 /CC). Subsequently, bioactive molecules (anti-25VD 3 and BSA) were introduced to fabricate the nanobioplatform BSA/anti-25VD 3 /nCeO 2 /CC as an immunosensor. The analytical performance of the developed immunosensor was studied towards 25VD 3 detection. The immunosensor provides a broad linear range of 1-200 ng mL -1 , high sensitivity of 2.08 μA ng −1 mL cm −2 , a detection limit of 4.63 ng mL −1 , and a response time of 15 min, which is better than that of previous reports. The biosensor exhibited high selectivity, good reproducibility, and excellent stability for about 45 days. The potential application of the proposed immunosensor was observed for real serum samples towards 25VD 3 detection that demonstrated a high correlation with the conventional enzyme-linked immunosorbent assay. Graphical abstract Supplementary Information The online version contains supplementary material available at 10.1007/s00604-021-04751-y.

A molecularly imprinted polymer based on a novel polyaniline–zinc sulfide nanocomposite for electrochemical detection of trimethylamine N-oxide

“…Also, the value of ΔEp [ΔEp = Epa − Epc] was evaluated to be 0.250 V and 0.273 V for the MIP/ITO and NIP/ITO electrodes, respectively. 58 Further, an increase in the peak potential with an increment in the scan rate shows a sluggish electron transfer at the interface.…”

“…Also, the value of ΔEp [ΔEp = Epa − Epc] was evaluated to be 0.250 V and 0.273 V for the MIP/ITO and NIP/ITO electrodes, respectively. 58 Further, an increase in the peak potential with an increment in the scan rate shows a sluggish electron transfer at the interface.ΔEp (MIP/ITO) = 0.020 V s −1 mV −1 × (scan rate [mV s −1 ] 1/2 ) + 0.101 V, R 2 = 0.995Similarly, linear fit curves of the NIP/ITO electrode showing the Ipa, Ipc, and ΔEp [Fig. S1(c)†] relationship as a function of the square root of the scan rate are given by eqn (5)–(7), respectively.Ipa (NIP/ITO) = 31.843 μA s −1 mV −1 × (scan rate [mV s −1 ] 1/2 ) + 118.359 μA, R 2 = 0.987Ipc (NIP/ITO) = −25.072 μA s −1 mV −1 × (scan rate [mV s −1 ] 1/2 ) + 71.380 μA, R 2 = 0.989ΔEp (NIP/ITO) = 0.023 V s −1 mV −1 × (scan rate [mV s −1 ] 1/2 ) + 0.102 V, R 2 = 0.997The analysis and electron movements were studied using kinetic interface parameters, for instance, the electron transfer rate constant (Ks), diffusion constant ( D ), surface area (Ae), and surface concentration of the redox probe of the electrode ( I *) for both MIP/ITO and NIP/ITO electrodes.…”

ASu@MNPs-based electrochemical immunosensor for vitamin D3 serum samples analysis

“…Therefore, for the best of our knowledge, our work presents the higher number of serum samples analyzed than any other experimental electrochemical immunosensor available in literature and, among the validated ones, the one exhibiting the lower limit of detection. MPA/Au DPV 20-200 ng ml −1 10 ng ml −1 0.16 A ml ng −1 cm-2 [6] CAEF/RCP* Chronoamperometry 10 -100 ng ml −1 10 ng ml −1 0.16 μA ml ng −1 cm −2 [40] nCeO2/CC** DPV 1-200 ng ml −1 4.63 ng ml −1 2.08 μA ml ng −1 cm −2 [41] Cys-Au@ZrO2/ITO DPV 1-50 ng ml −1 3.54 ng ml −1 2.01 μA ml ng −1 cm −2 [42] CD-CH***/ITO DPV 10-50 ng ml −1 1.35 ng ml −1 0.2 μA ml ng −1 cm-2 [43] Asp-Gd2O3NRs/ITO DPV 10-100 ng ml −1 0.10 ng ml −1 0.38 μA ml ng −1 cm-2 [11] Fe3O4-PANnFs/ITO DPV 10-100 ng ml −1 0.12 ng ml −1 0.90 μA ml ng −1 cm-2 [10] CYM@AuMNPs DPV 7.4 -70 ng ml −1 2.4 ng ml −1 0.55 μA ml ng −1 cm-2…”

Advances in electrochemical and optical sensing techniques for vitamins detection: a review

“…Cerium (IV) oxide (nCeO2) nanostructure was formed on carbon cloth (CC) by electrophoretic deposition, resulting in the nanoplatform (nCeO2/CC). Anti-25VD 3 and BSA on top of this, the BSA/ anti-25VD 3 /nCeO 2 /CC nanobioplatform enhanced Vit D 3 detection (Chauhan et al 2021). Aptamer with polyethyleneglycol (PEG)-free gold NR (AuNR) was used for sensing.…”