Gold Nanoparticles With Special Shapes: Controlled Synthesis, Surface-enhanced Raman Scattering, and The Application in Biodetection

Hu, Jianqiang; Wang, Zhouping; Li, Jinghong

doi:10.3390/s7123299

Cited by 164 publications

(95 citation statements)

References 41 publications

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“…XRD of synthesized GNP confirmed that the GNPs were in good agreement with previously reported data (Figure 2). 44,45 Photon cross-correlation spectroscopy (particle-size analyser) ( Figure 3D) and TEM findings ( Figure 3C) bolstered GNP size (22.4 and 21 ± 2.5 nm, respectively). Here, we used appropriate and optimum size and concentration (21 ± 2.5 nm and 0.2 mg/mL) of GNP, which is compatible with mammalian cells, as described elsewhere.…”

Section: Discussionmentioning

confidence: 99%

“…43 Figure 2 shows a typical powder XRD pattern of GNPs and confirmed the XRD peaks 111, 200, 220, and 311, which are in good agreement with previously reported data. 44,45 GNP size (21 ± 2.5 nm) and hydrodynamic mean diameter (22.4 nm) were analyzed and measured by TEM and cross-correlation spectroscopy instruments, respectively ( Figure 3C and D, respectively). It was observed that particles were ellipsoidal and quite stable as their sizes and shape remained constant after synthesis.…”

Section: Characterization Of Gnpmentioning

confidence: 99%

See 1 more Smart Citation

Gold nanoparticles enhance methylene blue– induced photodynamic therapy: a novel therapeutic approach to inhibit Candida albicans biofilm

Khan¹,

Alam²,

Azam³

et al. 2012

IJN

143

104

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This article explores the novel gold nanoparticle-enhanced photodynamic therapy of methylene blue against recalcitrant pathogenic Candida albicans biofilm. Physiochemical (X-ray diffraction, ultraviolet-visible absorption, photon cross-correlation, FTIR, and fluorescence spectroscopy) and electron microscopy techniques were used to characterize gold nanoparticles as well as gold nanoparticle-methylene blue conjugate. A 38.2-J/cm 2 energy density of 660-nm diode laser was applied for activation of gold nanoparticle-methylene blue conjugate and methylene blue against C. albicans biofilm and cells. Antibiofilm assays, confocal laser scanning, and electron microscopy were used to investigate the effects of the conjugate. Physical characteristics of the gold nanoparticles (21 ± 2.5 nm and 0.2 mg/mL) and methylene blue (20 µg/mL) conjugation were confirmed by physicochemical and electron microscopy techniques. Antibiofilm assays and microscopic studies showed significant reduction of biofilm and adverse effect against Candida cells in the presence of conjugate. Fluorescence spectroscopic study confirmed type I photo toxicity against biofilm. Gold nanoparticle conjugate-mediated photodynamic therapy may be used against nosocomially acquired refractory Candida albicans biofilm.

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Section: Discussionmentioning

confidence: 99%

Section: Characterization Of Gnpmentioning

confidence: 99%

Gold nanoparticles enhance methylene blue– induced photodynamic therapy: a novel therapeutic approach to inhibit Candida albicans biofilm

Khan¹,

Alam²,

Azam³

et al. 2012

IJN

143

104

View full text Add to dashboard Cite

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“…This peak is attributed to the surface plasmon resonance (SPR) due to the metallic gold nanoparticles embedded in the glass matrix. 10,11 There is no such absorption peak as seen in Fig. 1a.…”

Section: Resultsmentioning

confidence: 79%

Ionic to Electronic Conductivity Studies of Lithium-Borate Ruby-Glass-Ceramics Containing Gold Nano Particles

Shivaprakash

Anavekar

2011

Transactions of the Indian Ceramic Society

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Glass-ceramics containing Au nanoparticles have been synthesized in the glass composition 30Li 2 O-70B 2 O 3 with HAuCl 4 .3H 2 O as dopant. The characteristic ruby colour has been observed which is attributed to the surface plasmon resonance of the Au nanoparticles, uniformly embedded in the glass matrix. The imparted red colour due to the presence of gold nanoparticles has been confirmed by studying the optical absorption spectra using UV-VIS spectrometer in the visible range (400-1000 nm) which show a characteristic absorption peak at ~560 nm. In order to check the crystallization/ amorphous nature, the samples were powdered and characterized using X-ray diffraction (XRD). The microstructural modifications in the samples due to the addition of Au have been recorded using scanning electron microscopy (SEM). Further, the electron spin resonance (ESR) studies reveal that the electronic state of gold is either Au 0 or Au + . AC conductivity studies have been performed at room temperature over a frequency range 100 to 10 MHz. The ac conductivity data is fitted by Almond-West law with power exponent 's'. The dc conductivity is found to be increasing with the increase of temperature for a typical ruby glass, but almost constant with dopant concentration.[Keywords : Ruby-glass, Gold nanoparticles, Optical absorption, SPR, Transport properties] IntroductionRuby glasses containing gold nanoparticles or other noble metal nanoparticles have been extensively investigated for the past decades because of their excellent properties such as ultrafast optical response and large third order non-linear susceptibility. [1][2][3][4] This kind of glasses are expected to be promising materials for ultrafast optical switches and optical circuits with micrometer size. Hence, preparation and characterization of materials containing noble metal nanoparticles is the most challenging task for researchers in the area of materials science. Glasses containing metal nanoparticles look very distinctive with different colours. Since the quantum size effects and enhanced surface effects are expected to influence the electronic and optical properties at much smaller particle sizes, it leads to more interest for researchers to fabricate the metal nanoparticles in glasses. 5,6 Such investigated gold-ruby glasses are used not only as decorative glasswares but also for many technological applications in the field of electrical and optical communications. Silicate ruby glasses are synthesized mainly by using HAuCl 4 .3H 2 O along with a reducing agent, such as tin based compounds, and then annealing the glass for several hours at a particular temperature. 7,8 Metal nanoparticles in glasses are also precipitated using electron beam and laser irradiation. In these methods the metal ions are reduced but need thermal treatment.

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“…Gold and silver nanoparticles are commonly used as SERS substrates with gold particles being used more frequently due to their high sensitivity and stability compared to silver compounds [5][6][7][8]. It must be noted that the enhanced Raman signal enabled by the plasmon resonance of gold and silver nanoparticles gave rise to new biosensors that have been used for identifying the binding region in proteins [9], and for studying binding affinity of immunoreactions [10].…”

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confidence: 99%

Identification of binding interactions between myeloperoxidase and its antibody using SERS

et al. 2010

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Surface Enhanced Raman Spectroscopy (SERS) is a widely used spectroscopic method that can dramatically increase the sensitivity of Raman spectroscopy and has demonstrated significant benefit in the identification of biological molecules. We report the use of SERS in differentiating the bound immunocomplex of myeloperoxidase (MPO) and its antibody from the unbound complex and its individual components. The SERS signal was enabled by gold nanoparticles attached to MPO, pAb and their immunocomplex at an excitation wavelength of 785 nm. The obtained SERS spectrum of MPO is in agreement with previous literature. Comparative SERS spectrum analysis of MPO, pAb, and their immunocomplex reveals the significant peak shifts and intensity variations caused by the conformational changes due to the immunocomplex formation. Several key areas have been identified which correspond to specific amino acids being shielded from undergoing resonance while new amino acid residues are made visible in the SERS spectrum of the immunocomplex and could be a result of conformational binding. Our work demonstrates the capability of SERS to identify binding events and differentiate an immunocomplex from its unbound components with direct applications in biosensors.Keywords: Gold nanoparticle; Antigen; Immunocomplex; Biosensors Citation: E. S. Papazoglou, S. Babu, S. Mohapatra, D. R. Hansberry and C. Patel, "Identification of binding interactions between myeloperoxidase and its antibody using SERS", Nano-Micro Lett. 2, 74-81 (2010). doi:10.5101/nml.v2i2. p74-82 Raman spectroscopy is a useful technique, based on Raman scattering, that has been widely used for molecular material EXPERIMENTAL METHODSAll chemicals were purchased from Sigma Aldrich unless otherwise mentioned, pAb (rabbit anti human myeloperoxidase)was purchased from ABD-Serotec and myeloperoxidase (MPO) was purchased from Lee Biosolutions Inc.Gold nanoparticles were prepared according to Frens, G.[24] with added modifications. 500µl of 1% chloroauric acid Slides with immobilized gold nanoparticles were then washed with DI water and air dried. A well with a capacity of ~100 mL was constructed using plastic pipette and epoxy on top of the glass slide to facilitate pAb immobilization and subsequent SERS data collection. -Micro Lett. 2, 74-82 (2010) Polyclonal antibody was immobilized by letting ~100 ml of 100 nM pAb solutions interact with the gold nanoparticle coated slides for 15 minutes at room temperature followed by thorough washing with 1x PBS buffer (pH7.4). Gold nanoparticles coated with pAb were then allowed to interact with MPO (100 μl, 1 μM) Nanofor 15 minutes at room temperature followed by washing. A similar procedure was followed to immobilize MPO (100 μl, 1 M) directly on gold nanoparticles. SERS data was collected from the Au-pAb conjugates, Au-pAb/MPO immunocomplex, and Au-MPO conjugates immediately after preparation using a Renishaw RM1000 confocal Raman microspectrometer with a 50x long focal microscope. A 785nm diode laser (15 mW) was used to collect the ...

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Gold Nanoparticles With Special Shapes: Controlled Synthesis, Surface-enhanced Raman Scattering, and The Application in Biodetection

Cited by 164 publications

References 41 publications

Gold nanoparticles enhance methylene blue– induced photodynamic therapy: a novel therapeutic approach to inhibit Candida albicans biofilm

Gold nanoparticles enhance methylene blue– induced photodynamic therapy: a novel therapeutic approach to inhibit Candida albicans biofilm

Ionic to Electronic Conductivity Studies of Lithium-Borate Ruby-Glass-Ceramics Containing Gold Nano Particles

Identification of binding interactions between myeloperoxidase and its antibody using SERS

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Gold Nanoparticles With Special Shapes: Controlled Synthesis, Surface-enhanced Raman Scattering, and The Application in Biodetection

Cited by 164 publications

References 41 publications

Gold nanoparticles enhance methylene blue&ndash; induced photodynamic therapy: a novel therapeutic approach to inhibit Candida albicans biofilm

Gold nanoparticles enhance methylene blue&ndash; induced photodynamic therapy: a novel therapeutic approach to inhibit Candida albicans biofilm

Ionic to Electronic Conductivity Studies of Lithium-Borate Ruby-Glass-Ceramics Containing Gold Nano Particles

Identification of binding interactions between myeloperoxidase and its antibody using SERS

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Product

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Gold nanoparticles enhance methylene blue– induced photodynamic therapy: a novel therapeutic approach to inhibit Candida albicans biofilm

Gold nanoparticles enhance methylene blue– induced photodynamic therapy: a novel therapeutic approach to inhibit Candida albicans biofilm