Gold nanoparticles promote osteogenic differentiation of human periodontal ligament stem cells via the p38 MAPK signaling pathway

Niu, Chenguang; Yuan, Kai; Ma, Rui; Gao, Li; Jiang, Wenxin; Hu, Xuchen; Lin, Wei-Ming; Zhang, Xiaoling; Huang, Zhengwei

doi:10.3892/mmr.2017.7170

Cited by 50 publications

(41 citation statements)

References 46 publications

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“…It has been reported that during the process of adipocyte differentiation, cell morphology will change. With the change of cell morphology, the synthesis and assembly of actin and other major cytoskeleton proteins will also change [33], so the pathways involved in cell growth, proliferation, migration, and differentiation may affect lipid deposition. In fact, classical cell signaling pathways such as the MAPK signaling pathway and citrate cycle (TCA cycle) play an indispensable role in many cell responses, such as cell proliferation and differentiation [34,35].…”

Section: Discussionmentioning

confidence: 99%

MiRNAs and mRNAs Analysis during Abdominal Preadipocyte Differentiation in Chickens

Sun

Zhu

et al. 2020

Animals

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The excessive deposition of abdominal fat has become an important factor in restricting the production efficiency of chickens, so reducing abdominal fat deposition is important for improving growth rate. It has been proven that miRNAs play an important role in regulating many physiological processes of organisms. In this study, we constructed a model of adipogenesis by isolating preadipocytes (Ab-Pre) derived from abdominal adipose tissue and differentiated adipocytes (Ab-Ad) in vitro. Deep sequencing of miRNAs and mRNAs expressed in Ab-Pre and Ab-Ad groups was conducted to explore the effect of miRNAs and mRNAs on fat deposition. We identified 80 differentially expressed miRNAs (DEMs) candidates, 58 of which were up-regulated and 22 down-regulated. Furthermore, six miRNAs and six mRNAs were verified by qRT-PCR, and the results showed that the expression of the DEMs and differentially expressed genes (DEGs) in the two groups was consistent with our sequencing results. When target genes of miRNA were combined with mRNA transcriptome data, a total of 891 intersection genes were obtained, we predicted the signal pathways of cross genes enrichment to the MAPK signal pathway, insulin signal pathway, fatty acid metabolism, and ECM–receptor interaction. Meanwhile, we constructed miRNA and negatively correlated mRNA target networks, including 12 miRNA–mRNAs pairs, which showed a strong association with the abdominal adipocyte differentiation (miR-214−ACSBG2, NFKB2, CAMK2A, ACLY, CCND3, PLK3, ITGB2; miR-148a-5p−ROCK2; miR-10a-5p−ELOVL5; miR-146b-5p−LAMA4; miR-6615-5p−FLNB; miR-1774−COL6A1). Overall, these findings provide a background for further research on lipid metabolism. Thus, we can better understand the molecular genetic mechanism of chicken abdominal fat deposition.

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Section: Discussionmentioning

confidence: 99%

MiRNAs and mRNAs Analysis during Abdominal Preadipocyte Differentiation in Chickens

Sun

Zhu

et al. 2020

Animals

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“…The expression of osteogenic markers and alizarin red staining were upregulated by 100 nM FICZ. Several signalling pathways were reported to be involved in the study of agents promoting osteogenic differentiation of PDLCs, including JNK, p38 MARK and so on (Niu et al, ; Tang et al, ). Wnt/β‐catenin signalling pathway has been widely investigated in the osteogenesis process, and it was reported to have a crosstalk with AhR signalling in the development of zebrafish embryos (Wincent, Stegeman, & Jonsson, ).…”

Section: Discussionmentioning

confidence: 99%

Protective roles of FICZ and aryl hydrocarbon receptor axis on alveolar bone loss and inflammation in experimental periodontitis

Huang

Cai

et al. 2019

J Clinic Periodontology

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Aim: The aryl hydrocarbon receptor (AhR)-ligand axis has been shown to be involved in inflammatory diseases and bone homeostasis. However, the activation of AhR signalling pathway and the possible functions of AhR ligands in periodontitis are underexplored. This study investigated the expression of the AhR target gene cytochrome P450 subfamily B member 1 (CYP1B1) and the functions and mechanisms of the AhR ligand 6 formylindolo[3,2-b]carbazole (FICZ) in periodontitis. Materials and Methods: CYP1B1 expression was detected in human periodontitis samples, mice with ligature-induced periodontitis and lipopolysaccharide (LPS)-induced inflammation in periodontal ligament cells (PDLCs) in vitro. FICZ was administered topically or systemically. The therapeutic functions of FICZ were detected via qPCR, micro-computed tomography and immunohistochemistry. Finally, the mechanisms of AhR signalling in periodontitis were investigated by cell assays. Results: CYP1B1 expression was downregulated in periodontitis. FICZ rescued the alveolar bone loss and mitigated the inflammatory cytokines in periodontitis mice. In vitro, FICZ pre-treatment reduced the LPS-induced inflammation in PDLCs via the increased phosphorylation of STAT3. Additionally, FICZ prompted the mineralization of PDLCs via activation of the Wnt/β-catenin signalling pathway. Conclusion: AhR signalling pathway is suppressed in periodontitis and the AhR ligand FICZ can prevent periodontitis. K E Y W O R D S AhR signalling, CYP1B1, FICZ, inflammation, periodontitis | 883 HUANG et Al.

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“…The relative osteogenic gene expression value for control was one in this experiment. Collagen1 (COL1) is the most prominent protein that occurred in the bone matrix and generated during the proliferation of osteoblast cells [42]. The expression of the COL1 gene marker from hMSCs shows the formation of bone cells in the presence of G. frondosa.…”

Section: Mineralization and Real-time Pcr Analysismentioning

confidence: 99%

Influence of Maitake (Grifola frondosa) Particle Sizes on Human Mesenchymal Stem Cells and In Vivo Evaluation of Their Therapeutic Potential

Patel

Seo

Dutta

et al. 2020

BioMed Research International

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Maitake (Grifola frondosa) mushroom has received an enormous amount of attention as a dietary supplement due to its high nutritional values. The particle sizes of G. frondosa mushrooms were monitored by a classifying mill. β-Glucans are the bioactive component of the mushroom, and it was revealed through Fourier transform infrared spectroscopy (FTIR), proton and carbon nuclear magnetic resonance (1H and 13C-NMR), matrix-assisted laser desorption/ionization, and time-of-flight (MALDI-TOF) spectrometry. The biocompatibility of G. frondosa particles, as well as induced osteogenesis of hMSCs, was evaluated through WST-1 assay and alizarin staining (ARS) technique, respectively. Notably, enhanced cell viability was noted in the presence of G. frondosa. Significantly improved calcium deposition has observed from hMSCs with G. frondosa, suggesting to their mineralization potential. The expression of osteogenic related gene markers was examined in the presence of G. frondosa through real-time polymerase chain reaction (qPCR) technique. The upregulation of osteogenic gene markers in the presence of G. frondosa particles was indicating their superior osteogenic potential. Besides, G. frondosa also activated the secretion of various kinds of proteins from the hMSCs indicating their potential for tissue engineering applications. Enhanced secretion of different immunoglobulins was observed in rat serum in the presence of G. frondosa, further demonstrating their therapeutic nature. Therefore, G. frondosa is effective for enhanced osteogenesis and can be utilized as a natural, edible, and osteogenic agent.

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Gold nanoparticles promote osteogenic differentiation of human periodontal ligament stem cells via the p38 MAPK signaling pathway

Cited by 50 publications

References 46 publications

MiRNAs and mRNAs Analysis during Abdominal Preadipocyte Differentiation in Chickens

MiRNAs and mRNAs Analysis during Abdominal Preadipocyte Differentiation in Chickens

Protective roles of FICZ and aryl hydrocarbon receptor axis on alveolar bone loss and inflammation in experimental periodontitis

Influence of Maitake (Grifola frondosa) Particle Sizes on Human Mesenchymal Stem Cells and In Vivo Evaluation of Their Therapeutic Potential

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