Gold nanoparticles modulate the crosstalk between macrophages and periodontal ligament cells for periodontitis treatment

Ni, Can; Zhou, Ji; Kong, Na; Bian, Tianying; Zhang, Yangheng; Huang, Xiaofeng; Xiao, Yin; Yang, Wenrong; Yan, Fuhua

doi:10.1016/j.biomaterials.2019.03.039

Cited by 160 publications

(130 citation statements)

References 106 publications

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“…During such pathological cascades, many immune cells polarize toward proinflammatory responses; for example, we previously reported increased levels of M1 phenotypes of macrophages in inflammatory gingival tissues (Zhou et al, ). Given the nature of the disease, it is essential to regulate the immune response involved in periodontal reparative events when designing new therapies for periodontitis (e.g., see Bi et al, ; He et al, ; Ni et al, ; Wu et al, ; Yu, Wu, Yin, & Chen, ). Periodontal pathogens also activate T cells, especially CD4+ T cells (T‐helper cells), to develop into various subsets and initiate the acquired immune response (Knight, Liu, Seymour, Faggion, & Cullinan, ; Silva et al, ); subsequently, a spectrum of proinflammatory cytokines are released, which induces tissue damage and bone destruction (Hienz et al, ).…”

Section: Introductionmentioning

confidence: 99%

The relationship between T‐helper cell polarization and the RANKL/OPG ratio in gingival tissues from chronic periodontitis patients

Sun

et al. 2019

Clinical & Exp Dental Res

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This study aimed to investigate the relationship between inflammation‐related T‐helper cell polarization and the receptor activator for nuclear factor‐κB ligand (RANKL)/osteoprotegerin (OPG) ratio, which is associated with bone resorption or remodeling of chronic periodontitis patients. Gingival crevicular fluid (GCF) and gingival tissues were obtained from periodontally healthy individuals (PH group) and chronic periodontitis patients (CP group). The GCF levels of IFN‐γ, IL‐4, IL‐17, and IL‐10 linked to T‐helper cell polarization toward the Th1, Th2, Th17, and Treg phenotypes, respectively, were determined by ELISA. The expression levels of these cytokines and the polarized T‐helper cells in gingival tissues were assessed through immunohistochemical and immunofluorescence assays. In addition, the RANKL and OPG expression levels in gingival tissues were detected by immunohistochemical assays, and linear regression analysis was used to identify the potential relationship between T‐helper cell polarization and the RANKL/OPG ratio. In total, 22 individuals and 35 patients were enrolled in the present study. In both GCF and gingival tissues, increased levels of IL‐17 and the decreased levels of IL‐4 and IL‐10 were observed in the CP group. When polarized T‐helper cells were identified in gingival tissues, more Th1 and Th17 cells were found in the CP group, whereas more Th2 and Treg cells were found in the PH group. Although there was no significant difference in OPG expression between the two groups, the RANKL/OPG ratio in the CP group was higher than that in the PH group. The linear regression analysis showed that the presence of more Th1 and Th17 cells correlated with a higher RANKL/OPG ratio, whereas the presence of more Th2 cells correlated with a lower RANKL/OPG ratio. Th1 and Th17 cells are positively correlated and Th2 cells are negatively correlated with the RANKL/OPG ratio. Our data suggest that T‐helper cell polarization is closely linked to the RANKL/OPG ratio in gingival tissues from chronic periodontitis patients.

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Section: Introductionmentioning

confidence: 99%

The relationship between T‐helper cell polarization and the RANKL/OPG ratio in gingival tissues from chronic periodontitis patients

Sun

et al. 2019

Clinical & Exp Dental Res

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“…NF-κB induces the expression of cyclooxygenase-2 (COX-2) and transforming growth factor beta (TGF-β) [5]. Authors have reported that AuNps reduce the levels of inflammatory markers in experimental models of periodontitis [42], 1% carrageenan-induced peritonitis [20], and ethanol-methamphetamine-induced hepatic injury [7], and positively influence the cellular response to infection or inflammation, interfering with the balance of cytokines involved in the inflammatory process [43].…”

Section: Discussionmentioning

confidence: 99%

Effect of Gold Nanoparticle on 5-Fluorouracil-Induced Experimental Oral Mucositis in Hamsters

et al. 2020

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Background: Oral mucositis (OM) is a severe inflammation of the oral mucosal cells associated with chemotherapy and/or radiotherapy-induced toxicity, resulting in epithelial ulcers and higher risk of death from sepsis. The aim of the present study was to evaluate the nanoparticle (AuNp) effect on OM induced in hamsters. Materials and methods: 5-fluorouracil (5FU) was used on the first and second day of the experimental model in Golden sirian hamsters, and on the fourth day, mechanical trauma was applied to induce OM. The animals were divided into groups, i.e., polyvinylpyrrolidone (PVP), mechanical trauma (MT), 5FU, and groups treated with gold nanoparticles (AuNps) (62.5, 125, and 250 μg/kg). On the 10th day, animals were euthanized for macroscopic, histopathological, immunohistochemical, western blot, quantitative polymerase chain reaction (qRT-PCR), and AuNp quantification. Results: AuNp (250 μg/kg) reduced TNF-α, IL-1β, COX-2, NF-κB, TGF-β, and SMAD 2/3; increased glutathione levels; decreased the expression of Kelch ECH-associated protein 1 (KEAP1); and induced heme oxygenase 1 (HMOX-1) and NAD (P) H quinone oxidoreductase 1 (NQO1) genes. Conclusions: AuNp (250 μg/kg) prevented 5-FU-induced OM in hamsters and improved the parameters of inflammation and oxidative stress.

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“…In macrophages, various intracellular signalling pathways, such as the nuclear factor kappa B (NF-jB) pathway, have been found to be highly associated with the anti-inflammatory action of GNPs [34]. Herein, RAW264.7 cells were pretreated with GNP-CK-CopA3 (20 or 40 lg/mL) for 1 h and then irritated by LPS (1 mg/mL) for 2 h [35].…”

Section: Effects Of Gnp-ck-copa3 On the Nf-jb Signalling Pathwaymentioning

confidence: 99%

Intracellular synthesis of gold nanoparticles by Gluconacetobacter liquefaciens for delivery of peptide CopA3 and ginsenoside and anti-inflammatory effect on lipopolysaccharide-activated macrophages

Liu

Perumalsamy

Kang

et al. 2020

Artificial Cells, Nanomedicine, and Biotechnology

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Probiotic Gluconacetobacter strains are intestinal microbes with beneficial effects on human health. Recently, researchers have used these strains to biosynthesize metal and non-metal nanoparticles for treating various chronic diseases. Despite their importance in nanotechnology, gold nanoparticles (AuNPs) biosynthesized by Gluconacetobacter species have not been clearly identified for treating inflammation and inflammation-associated diseases. While ginsenoside CK has strong pharmaceutical activity, it also has strong cytotoxicity and hydrophobicity which is hurdle to make formulation. Peptide-nanoparticle hybrids are gaining increasing attention for their potential biomedical applications, including human inflammatory diseases. Herein, we developed peptide CopA3 surface conjugated and ginsenoside compound K (CK) loaded gold nanoparticles (GNP-CK-CopA3), which intracellularly synthesised by the probiotic Gluconacetobacter liquefaciens kh-1, to target lipopolysaccharide (LPS)-activated RAW264.7 macrophages. The synthetic GNP-CK-CopA3 was characterised by various instrumental techniques. The results of our cellular uptake and MTT assays exhibited obvious drug intracellular delivery without significant cytotoxicity. In addition, pre-treatment with GNP-CK-CopA3 significantly ameliorated LPS-induced nitric oxide (NO) and reactive oxygen species (ROS) production and suppressed the mRNA and protein expression of pro-inflammatory cytokines in macrophages. Furthermore, GNP-CK-CopA3 efficiently inhibited the activation of the nuclear factor-jB (NF-jB) and mitogen-activating protein kinase (MAPK) signalling pathways. Taken together, our findings highlight the potential of using peptide-nanoparticle hybrids in the development of anti-inflammatory approaches and providing the experimental foundation for further application.

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Gold nanoparticles modulate the crosstalk between macrophages and periodontal ligament cells for periodontitis treatment

Cited by 160 publications

References 106 publications

The relationship between T‐helper cell polarization and the RANKL/OPG ratio in gingival tissues from chronic periodontitis patients

The relationship between T‐helper cell polarization and the RANKL/OPG ratio in gingival tissues from chronic periodontitis patients

Effect of Gold Nanoparticle on 5-Fluorouracil-Induced Experimental Oral Mucositis in Hamsters

Intracellular synthesis of gold nanoparticles by Gluconacetobacter liquefaciens for delivery of peptide CopA3 and ginsenoside and anti-inflammatory effect on lipopolysaccharide-activated macrophages

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