Gold-Nanoparticles-Modified Cellulose Membrane Coupled with Laser Desorption/Ionization Mass Spectrometry for Detection of Iodide in Urine

Abstract

We report an efficient method for the determination of iodide (I(-)) ions by using gold-iodide hybrid cluster ions on gold nanoparticles (Au NPs) modified mixed cellulose ester membrane (Au NPs-MCEM) by pulsed laser desorption/ionization mass spectrometry (LDI-MS). When I(-) ions were deposited and concentrated on the surfaces of Au NPs (32 nm) via strong Au(+)-I(-) interaction on the MECM, the Au NPs-MCEM was observed to function as an efficient surface-assisted LDI substrate with very low background noise. When pulsed laser radiation (355 nm) was applied, I(-) binding to Au NPs ions induced the enhancement of the desorption and ionization efficiency of gold-iodide hybrid cluster ions from the Au NPs surfaces. The reproducibility of the probe for both shot-to-shot and sample-to-sample (both less than 10%) ion production was also improved by the homogeneous nature of the substrate surface. Thus, it allows the accurate and precise quantification of I(-) ions in high-salinity real samples (i.e., edible salt samples and urine) at the nanomolar range. This novel LDI-MS approach provides a simple route for the high-speed analysis of I(-) ions with high sensitivity and selectivity in real biological samples.

A Novel Sensor for Sensitive and Selective Detection of Iodide Using Turn-on Fluorescence Graphene Quantum Dots/Ag Nanocomposite

“…For example, deficiency of dietary iodine or abnormal metabolism of iodine could cause goiter, hypothyroidism, and hyperthyroidism. 1,2 Moreover, deficiency of dietary iodine could lead to developmental defects in extreme conditions. Therefore, the determination of iodide ions is valuable for epidemiological studies of iodine supplementation, particularly in diagnosing and controlling iodine deficiency.…”

Simultaneous and direct analysis of multiple types of organic contaminants in water based on a MOF decorated with a suitable quantity of Au nanoparticles, using SALDI-TOF MS

“…Gold nanoparticles (Au NPs) offer a powerful SALDI matrix for small biomolecules. [25][26][27][28][29][30][31] However, the Au NP matrix oen produces many Au cluster peaks in the mass spectrum that tend to suppress the actual sample ion peaks; moreover, the Au colloids may become inhomogeneously distributed aer being dried on the target plate, thus leading to poor reproducibility. To solve this problem, in our work, the Au NPs are loaded onto zirconium based metal-organic frameworks modied with amino groups (UiO-66-NH 2 ) via an impregnation method which takes advantage of the electrostatic attraction between AuCl 4 À and the amino groups on the MOF.…”

PolyA-tailed and fluorophore-labeled aptamer-gold nanoparticle conjugate for fluorescence turn-on bioassay using iodide-induced ligand displacement

“…On one hand, such two anions have been demonstrated to be capable of absorbing very strongly on gold surface for the removal of the surface impurities or other analytical applications (Chen et al, 2009;Li et al, 2013;Shen et al, 2014;Zhang et al, 2012c). Also, the Au-A interaction is intrinsically weaker than the Au-S covalent interaction (Zhang et al, 2012b), it is thus likely that a ligand displacement strategy of divide-and-conquer is adopted to displace the multiple consecutive adenines (polyA) anchored onto AuNP surface by the deposition of these anions on the particles ( Fig.…”