Gold nanoparticles based lateral flow immunoassay with largely amplified sensitivity for rapid melamine screening

Zhong, Youhao; Chen, Yinji; Li, Yao; Zhao, Diping; Zheng, Lei; Liu, Guodong; Ye, Yingwang; Chen, Wei

doi:10.1007/s00604-016-1812-9

Cited by 60 publications

(32 citation statements)

References 24 publications

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“…BSA-Au NPs-based lateral flow immunoassay was proposed by Zhong and co-workers For rapid discrimination of melamine [153]. This method was also legalised as an effective procedure for melamine detection, but still much focus is required to improve its applicability and detection limits.…”

Section: Nanoparticles In Melamine Quantificationmentioning

confidence: 99%

Review on Nanomaterial-Based Melamine Detection

Shellaiah

Sun

2019

Chemosensors

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Illegal adulteration of milk products by melamine and its analogs has become a threat to the world. In 2008, the misuse of melamine with infant formula caused serious effects on babies of China. Thereafter, the government of China and the US Food and Drug Administration (FDA) limited the use of melamine of 1 mg/kg for infant formula and 2.5 mg/kg for other dairy products. Similarly, the World Health Organization (WHO) has also limited the daily intake of melamine of 0.2 mg/kg body weight per day. Many sensory schemes have been proposed by the scientists for carrying out screening on melamine poisoning. Among them, nanomaterial-based sensing techniques are very promising in terms of real-time applicability. These materials uncover and quantify the melamine by means of diverse mechanisms, such as fluorescence resonance energy transfer (FRET), aggregation, inner filter effect, surface-enhanced Raman scattering (SERS), and self-assembly, etc. Nanomaterials used for the melamine determination include carbon dots, quantum dots, nanocomposites, nanocrystals, nanoclusters, nanoparticles, nanorods, nanowires, and nanotubes. In this review, we summarize and comment on the melamine sensing abilities of these nanomaterials for their suitability and future research directions.

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Section: Nanoparticles In Melamine Quantificationmentioning

confidence: 99%

Review on Nanomaterial-Based Melamine Detection

Shellaiah

Sun

2019

Chemosensors

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“…The systems that implement the aggregation of several types of functionalized nanoparticles cause particular interest. Such approaches are described, for example, by Choi et al [126] with a 100-fold gain in sensitivity for the detection of troponin I using two kinds of gold nanoparticles; by Razo et al [20] with the generation of an optical signal by complexes of iron oxide nanoparticles (also used as a concentrating agent) and gold nanoparticles with a 32-fold decrease in the detection limit of potato virus X; by Taranova et al [127] with a 30-fold gain in the analysis of procalcitonin due to biotin-streptavidin aggregation of gold nanoparticles; by Shi et al [128] with complexation of gold nanoparticles of two sizes in the analysis of imidaclothiz; by Zhong et al [129] with the formation of two layers of antibody conjugates with gold nanoparticles in the detection of melamine; and by Shen et al [130] with aggregation of gold nanoparticles using polyamidoamine dendrimer, which lowered the detection limit of rabbit immunoglobulin G 20 times.…”

Section: Proper Response For Lfiamentioning

confidence: 99%

Ways to Reach Lower Detection Limits of Lateral Flow Immunoassays

Zherdev¹,

Dzantiev²

2018

Rapid Test - Advances in Design, Format and Diagnostic Applications

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This chapter considers factors influencing sensitivity of lateral flow immunoassay and modern developments that are focused on reaching lower detection limits. The existing variety of proposed approaches is classified in accordance with the "big five rules" for these assays, including proper sample, receptor, interaction, response, and output. The solutions for rapid extraction of target analytes and preventing negative influence of extractants are considered. Role to antibodies affinity and specificity is characterized. Potential of alternate bioreceptor molecules is discussed. Immunoreactants' compositions, concentrations, and locations on the test strip are characterized as factors determining assay parameters. The existing variety of labels is compared in terms of their optical and alternate registration. Tools to modulate a sequence of analytical reactions and to form aggregates of the detected labels are considered. The discussed approaches are illustrated through developments of test strips for detection of mycotoxins, veterinary drugs, and other analytes.The overall design of the immunochromatographic test strip is shown in Figure 1. It is a composite of several membranes of different structures and porosities, fixed on a support. The bundling of the test strip can vary, so it makes sense to consider its design based on what analytical tasks are being performed on its different sites.A. Typically, the lower portion of the test strip contains a sample pad. It ensures the absorption of sample components, in which the presence of the target analyte is checked.B. The following is a section with immunoreagents that are washed out during the analysis and move upward along with the components of the sample. As a rule, a conjugate pad forms this zone. It contains a conjugate of antibodies against the target analyte with a nanodispersed label-particles of colored latex, colloidal gold, and so on.The next two sections are located on the main working membrane of the test strip. C.First, there is a zone along which the movement of the absorbed components of the sample and the washed immunoreagents continues. During this movement, immune reactions occur, and specific intermolecular complexes are formed. D.Next, a mixture of reacted and unreacted molecules enters the binding area with immobilized immunoreagents. Depending on whether the target analyte was present in the sample and in what amount, binding of labeled immune complexes occurs in certain areas (in the traditional case, with the formation of narrow colored lines). Usually, additional reagents are located here to control the functionality of the test system. E. The upper part of the test strip with the final pad, usually structurally similar to the sample pad, ensures the further movement of the reaction mixture under the action of capillary forces and the washing of unreacted components from the underlying areas. These processes allow the label's binding to be evaluated correctly.Membrane components of the test strip are fixed on a plastic support and partia...

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“…However, AuNPs-ICA is usually not sufficiently sensitive, and several efforts have been devoted toward sensitivity enhancement. [13][14][15] Among the novel labels applied in ICA (e.g., magnetic nanoparticles, upconverting phosphors, and time-resolved uorescence), quantum dots (QDs) have attracted considerable attention owing to their unique optical properties. QDs exhibit broad absorption spectra, narrow photoluminescence spectra, size tunability due to quantum connement, and excellent photostability.…”

Section: Introductionmentioning

confidence: 99%