Gold nanoparticles attenuate albuminuria by inhibiting podocyte injury in a rat model of diabetic nephropathy

Alomari, Ghada; Al‐Trad, Bahaa; Hamdan, Salehhuddin; Aljabali, Alaa A. A.; Zoubi, Mazhar Salim Al; Bataineh, Nesreen; Qar, Janti; Tambuwala, Murtaza M.

doi:10.1007/s13346-019-00675-6

Cited by 40 publications

(27 citation statements)

References 52 publications

(71 reference statements)

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“…AuNPs demonstrated antioxidant effects via quenching reactive oxygen species (ROS), such as superoxide anion radical (O 2 − ) and H 2 O 2 in a dose-dependent manner [27,30]. Other studies have also revealed anti-hyperglycemic, anti-inflammatory, and antioxidative activities of AUNPs in diabetic animals [24,31], collagen-induced arthritis [32], and bone loss models [33].…”

Section: Introductionmentioning

confidence: 99%

Nanogold Particles Suppresses 5-Flurouracil-Induced Renal Injury: An Insight into the Modulation of Nrf-2 and Its Downstream Targets, HO-1 and γ-GCS

et al. 2021

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The development of the field of nanotechnology has revolutionized various aspects in the fields of modern sciences. Nano-medicine is one of the primary fields for the application of nanotechnology techniques. The current study sheds light on the reno-protective impacts of gold nano-particles; nanoGold (AuNPs) against 5-flurouracil (5-FU)-induced renal toxicity. Indeed, the use of 5-FU has been associated with kidney injury which greatly curbs its therapeutic application. In the current study, 5-FU injection was associated with a significant escalation in the indices of renal injury, i.e., creatinine and urea. Alongside this, histopathological and ultra-histopathological changes confirmed the onset of renal injury. Both gene and/or protein expression of nuclear factor erythroid 2–related factor 2 (Nrf-2) and downstream antioxidant enzymes revealed consistent paralleled anomalies. AuNPs administration induced a significant renal protection on functional, biochemical, and structural levels. Renal expression of the major sensor of the cellular oxidative status Nrf-2 escalated with a paralleled reduction in the renal expression of the other contributor to this axis, known as Kelch-like ECH-associated protein 1 (Keap-1). On the level of the effector downstream targets, heme oxygenase 1 (HO-1) and gamma-glutamylcysteine synthetase (γ-GCS) AuNPs significantly restored their gene and protein expression. Additionally, combination of AuNPs with 5-FU showed better cytotoxic effect on MCF-7 cells compared to monotreatments. Thus, it can be inferred that AuNPs conferred reno-protective impact against 5-FU with an evident modulatory impact on Nrf-2/Keap-1 and its downstream effectors, HO-1 and γ-GCS, suggesting its potential use in 5-FU regimens to improve its therapeutic outcomes and minimize its underlying nephrotoxicity.

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Section: Introductionmentioning

confidence: 99%

Nanogold Particles Suppresses 5-Flurouracil-Induced Renal Injury: An Insight into the Modulation of Nrf-2 and Its Downstream Targets, HO-1 and γ-GCS

et al. 2021

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“…It is known that gold NPs (AuNPs) attenuate hyperglycemia in diabetic animal models without any observed side effects. Our research showed that AuNPs treatment prevented diabetes-associated increases in the BGL via the downregulation effect of AuNPs in renal mRNA or the protein expression of transforming growth factor β1 (TGF-β1), collagen IV, fibronectin, vascular endothelial growth factor-A (VEGF-A), and TNF-α [103]. In another study, the anti-hyperglycemic activity of AuNPs in STZ-induced diabetic animal models has been estimated.…”

Section: Contribution Of Authors To the Nano-based Diabetes Therapymentioning

confidence: 68%

Emerging Treatment Strategies for Diabetes Mellitus and Associated Complications: An Update

et al. 2021

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The occurrence of diabetes mellitus (DM) is increasing rapidly at an accelerating rate worldwide. The status of diabetes has changed over the last three generations; whereas before it was deemed a minor disease of older people but currently it is now one of the leading causes of morbidity and mortality among middle-aged and young people. High blood glucose-mediated functional loss, insulin sensitivity, and insulin deficiency lead to chronic disorders such as Type 1 and Type 2 DM. Traditional treatments of DM, such as insulin sensitization and insulin secretion cause undesirable side effects, leading to patient incompliance and lack of treatment. Nanotechnology in diabetes studies has encouraged the development of new modalities for measuring glucose and supplying insulin that hold the potential to improve the quality of life of diabetics. Other therapies, such as β-cells regeneration and gene therapy, in addition to insulin and oral hypoglycemic drugs, are currently used to control diabetes. The present review highlights the nanocarrier-based drug delivery systems and emerging treatment strategies of DM.

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“…The nephrin is essential for normal glomerular function. Preservation and permeability of the glomerular filtration barrier integrity is associated with the presence of proteins such as nephrin and podocin 22 .…”

Section: Discussionmentioning

confidence: 99%

L-carnitine effects in CCl4-nephrotoxicity: Immunohistochemical evaluation of glomerular nephrin and HIF-1alpha expressions

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et al. 2020

Cukurova Medical Journal

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In this study, we aimed to demonstrate the effects of L-carnitine after carbontetrachloride (CCl 4) toxicity through nephrin and Hypoxia inducible factor-1 alpha (HIF-1α) expressions in the glomerular structure. Materials and Methods: Forty male Sprague dawley rats were divided into 5 groups with animals in each group. Group I: Control group; 0.2 ml olive oil intraperitoneal (ip) twice weekly, Group II: L-carnitine group; 200 mg/kg Lcarnitine (ip) twice a week, Group III: CCl 4 group; 0.2 ml CCl 4 (ip) twice a week for 6 weeks, Group IV: L-carnitine + CCl 4 group, 200 mg/kg ip L-carnitine 24 hours before CCl 4 twice a week, Group V: CCl 4 + L-carnitine group; 200 mg/kg L-carnitine half an hour after CCl 4 twice a week. Immunohistochemical staining was performed on kidney tissue sections to show nephrin and HIF-1α expression. Expression densities of the proteins were measured by ImageJ program. Results: Nephrin expression was significantly increased in Group III compared to other groups. There was a significant increase in HIF-1α expression only between Group I and Group III. Expression densities of proteins in L-carnitine-treated groups were similar to control. Conclusion: L-carnitine has both protective and therapeutic effects against CCl 4 toxicity in renal glomeruli. Amaç: Bu çalışmada glomerül yapısındaki nefrin ve hipoksi indüklenebilir faktör-1alfa ekspresyonları aracılığıyla karbontetraklorid (CCl4) toksisitesi sonrası Lkarnitin'in etkilerinin gösterilmesini amaçladık. Gereç ve Yöntem: 40 adet Sprague dawley erkek sıçan 5 gruba (n=8) ayrıldı. Grup I: Kontrol grubu; 0.2 ml zeytinyağı intraperitoneal (ip) haftada 2 kez, Grup II: Lkarnitin grubu; 200 mg/kg L-karnitin (ip) haftada 2 kez, Grup III: CCl 4 group; 0.2 ml CCl 4 (ip) haftada 2 kez 6 hafta boyunca, Grup IV: L-karnitin + CCl 4 grubu, haftada 2 kez CCl 4 uygulamasından önce 200 mg/kg ip L-karnitin, Grup V: CCl 4 + L-karnitin grubu, haftada 2 kez CCl 4 uygulamasından 1 saat sonra 200 mg/kg ip L-karnitin. Böbrek doku kesitlerine nefrin ve HIF-1α ekspresyonunu göstermek için immunohistokimya boyama uygulandı. Proteinlerin ekspresyon yoğunlukları ImageJ programında ölçüldü. Bulgular: Nefrin ekspresyonu diğer gruplar ile kıyaslandığında Grup III'de anlamlı olarak arttı. HIF-1α ekspresyonu yalnızca Grup I ve Grup III arasında anlamlı şekilde arttı. Proteinlerin ekspresyon yoğunlukları Lkarnitin-tedavili gruplarda kontrol grubuna benzerdi. Sonuç: L-karnitin böbrek glomerulusunda CCl 4 toksisitesine karşı hem koruyucu hem tedavi edici etkilere sahiptir.

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Gold nanoparticles attenuate albuminuria by inhibiting podocyte injury in a rat model of diabetic nephropathy

Cited by 40 publications

References 52 publications

Nanogold Particles Suppresses 5-Flurouracil-Induced Renal Injury: An Insight into the Modulation of Nrf-2 and Its Downstream Targets, HO-1 and γ-GCS

Nanogold Particles Suppresses 5-Flurouracil-Induced Renal Injury: An Insight into the Modulation of Nrf-2 and Its Downstream Targets, HO-1 and γ-GCS

Emerging Treatment Strategies for Diabetes Mellitus and Associated Complications: An Update

L-carnitine effects in CCl4-nephrotoxicity: Immunohistochemical evaluation of glomerular nephrin and HIF-1alpha expressions

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