Gold nanoparticles assisted sensitivity improvement of interdigitated microelectrodes biosensor for amyloid-β detection in plasma sample

Yoo, Yong Kyoung; Kim, Gangeun; Park, Dongsung; Kim, Jinsik; Kim, Young Soo; Kim, Hye Yun; Yang, Seung Dae; Lee, Jeong Hoon; Hwang, Kyo Seon

doi:10.1016/j.snb.2020.127710

Cited by 25 publications

(18 citation statements)

References 31 publications

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“…Regarding chronoamperometric biosensors, antibodies [ 8 , 9 , 10 ], aptamers [ 11 ], peptides [ 12 ] and enzymes [ 13 ] have been used as recognition molecules, and they are based on dual potentiometry. Regarding impedimetric biosensors using IDE, antibodies [ 24 , 25 , 26 , 27 , 28 ] and aptamers [ 29 ] have been used as the primary molecular recognition elements, as affinity-based detection requires strong binding and selective biomolecules for impedance measurement. With impedimetric biosensors, measurement is based on the access of a redox probe to the electrode surface, and changes occur when an insulating layer is produced with the recognition element/antigen complex, which reduces the transfer of electrons.…”

Section: Resultsmentioning

confidence: 99%

Development of an Interdigitated Electrode-Based Disposable Enzyme Sensor Strip for Glycated Albumin Measurement

et al. 2021

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Glycated albumin (GA) is an important glycemic control marker for diabetes mellitus. This study aimed to develop a highly sensitive disposable enzyme sensor strip for GA measurement by using an interdigitated electrode (IDE) as an electrode platform. The superior characteristics of IDE were demonstrated using one microelectrode of the IDE pair as the working electrode (WE) and the other as the counter electrode, and by measuring ferrocyanide/ferricyanide redox couple. The oxidation current was immediately reached at the steady state when the oxidation potential was applied to the WE. Then, an IDE enzyme sensor strip for GA measurement was prepared. The measurement of fructosyl lysine, the protease digestion product of GA, exhibited a high, steady current immediately after potential application, revealing the highly reproducible measurement. The sensitivity (2.8 nA µM−1) and the limit of detection (1.2 µM) obtained with IDE enzyme sensor strip were superior compared with our previously reported sensor using screen printed electrode. Two GA samples, 15 or 30% GA, corresponding to healthy and diabetic levels, respectively, were measured after protease digestion with high resolution. This study demonstrated that the application of an IDE will realize the development of highly sensitive disposable-type amperometric enzyme sensors with high reproducibility.

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Section: Resultsmentioning

confidence: 99%

Development of an Interdigitated Electrode-Based Disposable Enzyme Sensor Strip for Glycated Albumin Measurement

et al. 2021

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“…Surface plasmon resonance approaches to include nanoparticles for the detection of Aβ and, to a lesser extent, of α-synuclein 77 . Nanoplatforms induced gold and silver nanoparticles designed for biomedical applications, especially for the innovation of new strategies for molecular diagnosis with higher analytical capability, sensitivity with the possibility to use for neuropsychiatry 78 . These nanodiagnostics tools possess very good optical and spectral properties i.e.…”

Section: Nanotheranostics Induced Molecular Imaging Diagnosis Neuropmentioning

confidence: 99%

Review of nanotheranostics for molecular mechanisms underlying psychiatric disorders and commensurate nanotherapeutics for neuropsychiatry: The mind knockout

Kumar¹,

Chhikara²,

Gulia³

et al. 2021

Nanotheranostics

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Bio-neuronal led psychiatric abnormalities transpired by the loss of neuronal structure and function (neurodegeneration), pro-inflammatory cytokines, microglial dysfunction, altered neurotransmission, toxicants, serotonin deficiency, kynurenine pathway, and excessively produced neurotoxic substances. These uncontrolled happenings in the etiology of psychiatric disorders initiate further changes in neurotransmitter metabolism, pathologic microglial, cell activation, and impaired neuroplasticity. Inflammatory cytokines, the outcome of dysfunctional mitochondria, dysregulation of the immune system, and under stress functions of the brain are leading biochemical factors for depression and anxiety. Nanoscale drug delivery platforms, inexpensive diagnostics using nanomaterials, nano-scale imaging technologies, and ligand-conjugated nanocrystals used for elucidating the molecular mechanisms and foremost cellular communications liable for such disorders are highly capable features to study for efficient diagnosis and therapy of the mental illness. These theranostic tools made up of multifunctional nanomaterials have the potential for effective and accurate diagnosis, imaging of psychiatric disorders, and are at the forefront of leading technologies in nanotheranostics openings field as they can collectively and efficiently target the stimulated territories of the cerebellum (cells and tissues) through molecular-scale interactions with higher bioavailability, and bio-accessibility. Specifically, the nanoplatforms based neurological changes are playing a significant role in the diagnosis of psychiatric disorders and portraying the routes of functional restoration of mental disorders by newer imaging tools at nano-level in all directions. Because of these nanotherapeutic platforms, the molecules of nanomedicine can penetrate the Blood-Brain Barrier with an increased half-life of drug molecules. The discoveries in nanotheranostics and nanotherapeutics inbuilt unique multi-functionalities are providing the best multiplicities of novel nanotherapeutic potentialities with no toxicity concerns at the level of nano range.

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“…AuNPs have also been used as labels for AD peptide biomarkers sensing [ 105 , 106 , 107 , 108 , 109 , 110 , 111 ]. Yoo et al [ 106 ] designed a sandwich-type immunosensor for the detection of Aβ peptide by using an antibody-modified interdigitated microelectrode and AuNPs conjugated with antibodies as labels for signal amplification ( Figure 7 A). By measuring impedance changes in the presence of Aβ peptides, they obtained a LOD of 22.15 fM, what indicates that the use of AuNPs as labels for Aβ peptide is as valid as the use of AuNPs as electrode modifiers [ 94 ].…”

Section: Electrochemical Biosensors For Alzheimer’s Disease Diagnomentioning

confidence: 99%

“…The reduction process of thionine was electrochemically monitored for amplifying the signal recorded by DPV, providing a LOD of 100 pM. The use of thionine as electrochemical target seems to be less effective than AuNPs alone, as it was the case of Yoo et al [ 106 ]. In this case, the difference between the analytical methods followed (impedance changes and DPV) may also be a determining factor that should be taken into consideration.…”

Section: Electrochemical Biosensors For Alzheimer’s Disease Diagnomentioning

confidence: 99%

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Electrochemical Biosensors Based on Nanomaterials for Early Detection of Alzheimer’s Disease

Toyos-Rodríguez

Alonso

Escosura‐Muñiz

2020

Sensors

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Alzheimer’s disease (AD) is an untreatable neurodegenerative disease that initially manifests as difficulty to remember recent events and gradually progresses to cognitive impairment. The incidence of AD is growing yearly as life expectancy increases, thus early detection is essential to ensure a better quality of life for diagnosed patients. To reach that purpose, electrochemical biosensing has emerged as a cost-effective alternative to traditional diagnostic techniques, due to its high sensitivity and selectivity. Of special relevance is the incorporation of nanomaterials in biosensors, as they contribute to enhance electron transfer while promoting the immobilization of biological recognition elements. Moreover, nanomaterials have also been employed as labels, due to their unique electroactive and electrocatalytic properties. The aim of this review is to add value in the advances achieved in the detection of AD biomarkers, the strategies followed for the incorporation of nanomaterials and its effect in biosensors performance.

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Gold nanoparticles assisted sensitivity improvement of interdigitated microelectrodes biosensor for amyloid-β detection in plasma sample

Cited by 25 publications

References 31 publications

Development of an Interdigitated Electrode-Based Disposable Enzyme Sensor Strip for Glycated Albumin Measurement

Development of an Interdigitated Electrode-Based Disposable Enzyme Sensor Strip for Glycated Albumin Measurement

Review of nanotheranostics for molecular mechanisms underlying psychiatric disorders and commensurate nanotherapeutics for neuropsychiatry: The mind knockout

Electrochemical Biosensors Based on Nanomaterials for Early Detection of Alzheimer’s Disease

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