Gold nanoparticle-based colorimetric platform technology as rapid and efficient bacterial pathogens detection method from various sources

Abstract

Rapid, sensitive, and reliable bacterial pathogens detection is a chief requirement. The gold nanoparticles (AuNPs) have numerous applications such as in the detection of biomolecules for their high surface to volume ratio and unique optical property facilitating development of highly efficient AuNPs-based bio-sensing tools. Although various molecular detection methods, such as PCR, real-time PCR, and loop-mediated isothermal amplification are sensitive and convenient, these techniques need elaborate work and require special skills to increase their specificity. Smartly fabricated gold nanoparticle (GNPs) play a role as probes for selective detection of pathogens. The AuNPs-based colorimetric methods have become applicable for rapid, simple, reliable and high-efficient, sensitive, inexpensive, and easy detection of the DNA, RNA, and protein biomolecules. Colorimetric detection using AuNPs has been used for rapid and high precision and multiplex detection of a large number and of bacterial pathogens. AuNPs act in functionalized and unfunctionalized ways. AuNPs-based colorimetric methods have incredible advantages compared with many other bacterial detection methods. In spite of many molecular techniques, AuNPs-based colorimetric methods do not require additional devices, fabrication cost, signal processing and interpretation complexities, and costly and complex instruments. This simple and rapid method is suitable, especially in low-income areas and for large number of samples analysis. In this review, applications of AuNPs and AuNPs-based colorimetric methods for bacterial pathogens detection have been overviewed.

Establishment and application of a gold nanoparticle-based immunochromatographic test strip for the detection of avian leukosis virus P27 antigen in egg white samples

“…Too much mAbs-labeled AuNPs will not only cause waste, but also cause tailing of the test strip; insufficient labeling of the mAbs leads to incomplete labeling of the AuNPs, which reduces the sensitivity of the test strip and the occurrence of false positives. 38,39 Additionally, the antibody on the T line and C line directly affects the binding ratio of the antigen or antibody to the mAbs-labeled AuNPs, and ultimately affects the color development of test strip. If the concentration of capture antibody is too low, the color of the band on the membrane is not clear or the band is hollow.…”

Nanotechnology for Therapy of Zoonotic Diseases: A Comprehensive Overview

“…Prasad and colleagues successfully targeted the Salmonella enterotoxin gene ( Stn ) by utilizing AuNPs to improve the LOD compared to electrophoresis gels. As a result, the signal strength was improved, and the Stn gene was easily detected [101] …”

“…As a result, the signal strength was improved, and the Stn gene was easily detected. [101] There are numerous nucleic acid-based techniques used for the detection of Salmonella. Selectively targeting antibodies that can selectively target molecules on the surface of bacteria have the potential to detect the pathogen precisely.…”

GLAD Based Advanced Nanostructures for Diversified Biosensing Applications: Recent Progress

“…Their biocompatibility and efficient thiol binding ability make gold nanoparticles (AuNPs) an ideal candidate for rapid biosensing based on the sensitivity of the surface plasmon resonance to the aggregation state, which produces a visible color change. Additionally, therefore, antibody-conjugated AuNPs have been used in the rapid colorimetric detection of pathogenic bacterial species in multiple systems, [ 188 , 189 , 190 , 191 ] Huan et al [ 191 ] have developed a simple phage display for calorimetric detection of a variety of bacterial species, ( Figure 16 ). This stage involeves Au nanoparticles for simple, efficient and sensitive detection of bacterial cells upto 100 cells with no cross reactivity between different species.…”