Gold nanoparticle-assisted all optical localized stimulation and monitoring of Ca2+ signaling in neurons

Lavoie-Cardinal, Flavie; Salesse, Charleen; Bergeron, Éric; Meunier, Michel; Koninck, Paul De

doi:10.1038/srep20619

Cited by 57 publications

(47 citation statements)

References 34 publications

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“…This magnetothermal approach to temporally and spatially control release of compounds in the brain may perhaps be compatible with other neuromodulation techniques that rely upon light in the visible (optogenetics) or possibly near infrared (direct or with plasmonic nanostructures) spectral range. The option of using such a tool alongside other stimuli could ultimately enable neuroscientists to design experiments that probe functional connectivity between distinct brain structures.…”

Section: Resultsmentioning

confidence: 95%

Localized Excitation of Neural Activity via Rapid Magnetothermal Drug Release

Romero

Christiansen

Barbosa

et al. 2016

Adv Funct Materials

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Hysteretic heat dissipation by magnetic nanoparticles (MNPs) in alternating magnetic fields (AMFs) allows these materials to act as local transducers of external stimuli. Commonly employed in cancer research, MNPs have recently found applications in remote control of heat‐dependent cellular pathways. Here, a thermally labile linker chemistry is adapted for the release of neuromodulatory compounds from the surfaces of MNPs via local nanoscale heating. By examining a range of MNP sizes, and considering individual particle loss powers, AMF conditions and nanomaterials suitable for rapid and complete release of a payload from MNP surfaces are selected. Local release of allyl isothiocyanate, an agonist of the Ca2+ channel TRPV1 (transient receptor potential vanilloid cation channel subfamily member 1), from iron oxide MNPs results in pharmacological excitation of neurons with latencies of ≈12 s. When targeted to neuronal membranes, these MNPs trigger Ca2+ influx and action potential firing at particle concentrations three orders of magnitude less than those previously used for magnetothermal neuromodulation accomplished with bulk heating.

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Section: Resultsmentioning

confidence: 95%

Localized Excitation of Neural Activity via Rapid Magnetothermal Drug Release

Romero

Christiansen

Barbosa

et al. 2016

Adv Funct Materials

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“…Media was replaced 1:1 every three days and cells were used at 7 DIV unless stated otherwise. For live STED recordings, rat hippocampal neurons were prepared as described previously 33 .…”

Section: Primary Neuronal Culturesmentioning

confidence: 99%

Nano-positioning and tubuline conformation determine transport of mitochondria along microtubules

Steenbergen

Lavoie-Cardinal

Kazwiny³

et al. 2020

Preprint

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Correct spatiotemporal distribution of organelles and vesicles is crucial for healthy cell functioning and is regulated by intracellular transport mechanisms. Controlled transport of bulky mitochondria is especially important in polarized cells such as neurons that rely on these organelles to locally produce energy and buffer calcium.Mitochondrial transport requires and depends on microtubules which fill much of the available axonal space. How mitochondrial transport is affected by their position within the microtubule bundles is not known. Here, we found that anterograde transport, driven by kinesin motors, is susceptible to the molecular conformation of tubulin both in vitro and in vivo. Anterograde velocities negatively correlate with the density of elongated tubulin dimers, similar to GTP-tubulin, that are more straight and rigid. The impact of the tubulin conformation depends primarily on where a mitochondrion is positioned, either within or at the rim of microtubule bundle. Increasing elongated tubulin levels lowers the number of motile anterograde mitochondria within the microtubule bundle and increases anterograde transport speed at the microtubule bundle rim. We demonstrate that the increased kinesin step processivity on microtubules consisting of elongated dimers underlies increased mitochondrial dynamics. Our work indicates that the molecular conformation of tubulin controls mitochondrial motility and as such locally regulates the distribution of mitochondria along axons.

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“…Both spontaneous and stimulated spiking activities provide unique information on the interactions of ion channels and synapses from single neurons to complex networks 5,6 . For decades, a number of techniques have been developed to record action potentials, including patch-clamps 7,8 , optical-probes 3,9,10 , multi-electrode arrays 6,11,12 , and several types of optical imaging modalities 4,5,10,11 . In particular, single/two-photon fluorescence microscopy has become one of the most powerful and popular approaches to monitoring ion dynamics of neuronal activities in large networks.…”

Section: Introductionmentioning

confidence: 99%

Laser Recording of Subcellular Neuron Activities

Chen

Zhu

et al. 2019

Preprint

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Advances in imaging and recording of neural activities with a single neuron resolution have played a significant role in understanding neurological diseases in the past decade. Conventional methods relying on patch-clamp and electrodes are regarded as invasive, whereas fluorescence-based imaging tools are useful but still suffer from a low signal-to-noise ratio and low sensitivity. Here we developed a novel optical imaging and recording system by employing laser emissions to record the action potentials in single neurons and neuronal networks caused by subtle transients (Ca2+concentration) in primary neuronsin vitrowith a subcellular and single-spike resolution. By recording the laser emissions from neurons, we discovered that lasing emissions could be biologically modulated by intracellular activities and extracellular stimulation with >100-fold improvement in detection sensitivity over traditional fluorescence-based measurement. Finally, we showed that ultrasound can wirelessly activate neurons adsorbed with piezoelectric BaTiO3nanoparticles, in which the neuron laser emissions were modulated by ultrasound. Our findings show that ultrasound stimulation can significantly increase the lasing intensity and neuron network response. This work not only opens the door to laser emission recording of intracellular dynamics in neuronal networks but may provide an ultra-sensitive detection method for brain-on-chip applications, optogenetics, and neuro-analysis.

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Gold nanoparticle-assisted all optical localized stimulation and monitoring of Ca2+ signaling in neurons

Cited by 57 publications

References 34 publications

Localized Excitation of Neural Activity via Rapid Magnetothermal Drug Release

Localized Excitation of Neural Activity via Rapid Magnetothermal Drug Release

Nano-positioning and tubuline conformation determine transport of mitochondria along microtubules

Laser Recording of Subcellular Neuron Activities

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