Gold Nanoislands Grown on Multiphoton Polymerized Structures as Substrate for Enzymatic Reactions

Abstract

We show that multiphoton thiol–ene polymerized structures comprise unreacted thiol moieties, which can be used for postpolymerization gold metallization. Some of the thiol groups located at the surface are not involved in thiol–ene reactions and, therefore, can serve as nucleation seeds for the synthesis of ∼50 nm sized gold nanoislands. Additionally, we show that the nanoislands can be used for immobilization of fluorescent molecules. We observed a significant enhancement of the fluorescence signal on the nanoisland-functionalized polymer structures when compared to the structured polymers without gold. To show a possible application, we bound peroxidase to the gold nanoislands. Peroxidase activity has been verified by chemiluminescence of the converted luminol substrate.

Strategies for Integrating Metal Nanoparticles with Two‐Photon Polymerization Process: Toward High Resolution Functional Additive Manufacturing

“…2PP with thiol‐ene and thiol‐yne resins have been used to build 3D mesostructures [ 37 ] and thiol functionality is also known to have high affinity to metal nanoparticles such as AuNPs. [ 38 ] Here we demonstrate that multimaterial 2PP with PETA and thiol‐ene resins offer a route for fabrication of intricate geometries with sub‐micron resolution as well as to achieve selective decoration of complex and curved surfaces. This facile methodology for selective Au patterning of micro/nanoscale surfaces, including curved and intricately designed surfaces, opens up new opportunities for micro/nano plasmonic metal patterns for applications such as sensing and metamaterials.…”

Single molecule studies of dynamic platelet interactions with endothelial cells

“…To gain spatio-temporal control of cellular stress, single EC (ltECs) nuclei were treated with a femtosecond pulsed laser. Experiments (N = 11, three to five ECs treated each) targeting single ECs were carried out using a 515 nm laser (290 fs pulse duration, 1 MHz repetition rate) with a peak power of 1,600 W/μm³ and an air objective lens (50x, NA = 0.42) ( Buchegger et al, 2019 ; Buchegger et al, 2021 ; Naderer et al, 2024 ). During laser treatment, ltECs exhibited morphological changes, nucleoplasm leakage, as well as occasionally nuclear bleb formation ( Figure 2B ).…”

Dual Channel Microfluidics for Mimicking the Blood–Brain Barrier

“…As shown in the fluorescence image of the polymer structure (Figure S1f), streptavidin efficiently bound to the biotinylated PETA containing the ring substructure and did not bind to the protein repellent rectangular grid consisting of PETA–PEGDA. Such selective polymer functionalization ,− enables local immobilization of biomolecules inside the imaging window and could potentially be used for cell-type-specific stimulation. ,, …”