Gold nano-urchin integrated label-free amperometric aptasensing human blood clotting factor IX: A prognosticative approach for “Royal disease”

Letchumanan, Iswary; Gopinath, Subash C. B.; Arshad, M. K. Md; Anbu, Periasamy; Lakshmipriya, Thangavel

doi:10.1016/j.bios.2019.02.006

Cited by 75 publications

(58 citation statements)

References 18 publications

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“…The concentrations from the lower to the higher picomolar range concentrations (3, 6, 50, 100, 125 pM). The current flow was noted to compare the performance between nanogapped sensor and Interdigitated Electrode (IDE) sensor comprises a couple of electrodes, which are arranged in a comb-like fashion 41 . Figure 7 shows the current flow when using the nanogapped sensor.…”

Section: Surface Physical Characterizationmentioning

confidence: 99%

Comparative Analysis on Dielectric Gold and Aluminium Triangular Junctions: Impact of Ionic Strength and Background Electrolyte by pH Variations

Letchumanan

Arshad

Gopinath

et al. 2020

Sci Rep

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Field of generating a surface thin film is emerging broadly in sensing applications to obtain the quick and fast results by forming the high-performance sensors. Incorporation of thin film technologies in sensor development for the better sensing could be a promising way to attain the current requirements. This work predominantly delineates the fabrication of the dielectric sensor using two different sensing materials (Gold and Aluminium). conventional photolithography was carried out using silicon as a base material and the photo mask of the dielectric sensor was designed by AutocAD software. the physical characterization of the fabricated sensor was done by Scanning electron Microscope, Atomic Force Microscope, High Power Microscope and 3D-nano profiler. The electrical characterization was performed using Keithley 6487 picoammeter with a linear sweep voltage of 0 to 2 V at 0.01 V step voltage. By pH scouting, i-V measurements on the bare sensor were carried out, whereby the gold electrodes conducts a least current than aluminium dielectrodes. comparative analysis with pH scouting reveals that gold electrode is suitable under varied ionic strengths and background electrolytes, whereas aluminium electrodes were affected by the extreme acid (pH 1) and alkali (pH 12) solutions.

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Section: Surface Physical Characterizationmentioning

confidence: 99%

Comparative Analysis on Dielectric Gold and Aluminium Triangular Junctions: Impact of Ionic Strength and Background Electrolyte by pH Variations

Letchumanan

Arshad

Gopinath

et al. 2020

Sci Rep

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View full text Add to dashboard Cite

show abstract

“…e fabrication of the IDE sensing surface was followed as stated in previous methods using different parameters with chemical and physical surface modifications [23]. Initially, the silicon wafers were oxidized at high temperature, and then, an etching process was carried out with aluminum.…”

Section: Ide Sensing Surface Preparationmentioning

confidence: 99%

Coordination of Nanoconjugation with an Antigen/Antibody for Efficient Detection of Gynecological Tumors

Liu

Yang

Shao

et al. 2020

Journal of Analytical Methods in Chemistry

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Cervical, ovarian, and endometrial cancers are common in the female reproductive system. Cervical cancer starts from the cervix, while ovarian cancer develops when abnormal cells grow in the ovary. Endometrial or uterine cancer starts from the lining of the womb in the endometrium. Approximately 12,000 women are affected every year by cervical cancer in the United States. Squamous cell carcinoma antigen (SCC-Ag) is a well-established biomarker in serum for diagnosing gynecological cancers, and its levels were observed to be elevated in cervical, ovarian, and endometrial cancer patients. Moreover, SCC-Ag was used to identify the tumor size and progression stages. Various biosensing systems have been proposed to identify SCC-Ag; herein, enhanced interdigitated electrode sensing is presented with the use of gold nanoparticles (GNPs) to conjugate an antigen/antibody. It was proved that the limit of detection is 62.5 fM in the case of antibody-GNP, which is 2-fold higher than that by SCC-Ag-GNP. Furthermore, the antibody-GNP-modified surface displays greater current increases with concomitant dose-dependent SCC-Ag levels. High analytical performance was shown by the discrimination against α-fetoprotein and CYFRA 21-1 at 1 pM. An enhanced sensing system is established for gynecological tumors, representing an advance from the earlier detection methods.

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“…The sensor (interdigitated electrode) proposed in this study has the potential for highperformance analysis with a wide range of biomarkers. It is a dielectrode system with alternate gaps and fingers that operate under dielectric measurements [13][14][15].…”

Section: Introductionmentioning

confidence: 99%

Detection of microRNA-335-5p on an Interdigitated Electrode Surface for Determination of the Severity of Abdominal Aortic Aneurysms

Liu

Wang

et al. 2020

Nanoscale Res Lett

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Abdominal aortic aneurysm (AAA) refers to the enlargement of the lower artery of the abdominal aorta, and identification of an early detection tool is urgently needed for diagnosis. In the current study, an interdigitated electrode (IDE) sensing surface was used to identify miRNA-335-5p, which reflects the formation of AAAs. The uniformity of the silica material was observed by 3D profilometry, and the chemically modified highly conductive surface improved the detection via the I-V mode. The targeted miRNA-335-5p was detected in a dose-dependent manner and based on linear regression and 3σ analyses, the sensitivity was determined to be 1 fM with a biotinylated probe. The high specificity was shown by discriminating the target sequence from noncomplementary and single-and triple-mismatched sequences. These outputs demonstrated the high-performance detection of miRNA-335-5p with good reproducibility for determination of the severity of AAA.

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Gold nano-urchin integrated label-free amperometric aptasensing human blood clotting factor IX: A prognosticative approach for “Royal disease”

Cited by 75 publications

References 18 publications

Comparative Analysis on Dielectric Gold and Aluminium Triangular Junctions: Impact of Ionic Strength and Background Electrolyte by pH Variations

Comparative Analysis on Dielectric Gold and Aluminium Triangular Junctions: Impact of Ionic Strength and Background Electrolyte by pH Variations

Coordination of Nanoconjugation with an Antigen/Antibody for Efficient Detection of Gynecological Tumors

Detection of microRNA-335-5p on an Interdigitated Electrode Surface for Determination of the Severity of Abdominal Aortic Aneurysms

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