Gold-copper nanoshell dot-blot immunoassay for naked-eye sensitive detection of tuberculosis specific CFP-10 antigen

Recent applications and strategies in nanotechnology for lung diseases

“…The method can detect CFP-10 antigen in clinical urine samples with high detection sensitivity and specificity. This method can be used for accurate and reliable real-time detection of CFP-10 and is significantly valuable for the early diagnosis of tuberculosis [163].…”

Advanced Signal-Amplification Strategies for Paper-Based Analytical Devices: A Comprehensive Review

“…An alternative way to prepare AuNP aggregates is based on a high concentration of salt in the reaction Ref. [48]. (B) The mechanism for detection of pore-forming toxin Listeriolysin O (LLO) using AuNP aggregation-based signal enhancement.…”

“…The subsequent addition of sodium ascorbate (SA) leads to reduction of the forming complex, enabling the formation of a well-defined Cu nanopolyhedron shell that is quantitatively detectable with the naked eye [ 47 ]. This approach was successfully employed by Phan et al to prepare Cu nanoshells on AuNP surfaces in dot-blot immunoassays for sensitive detection of the Mycobacterium tuberculosis –specific antigen CFP-10 [ 48 ]. The fabrication process is illustrated in Figure 2 A.…”

Nanomaterials for Healthcare Biosensing Applications

“…Novel inorganic architectures such as nanoshells, nanocages, and nanowires have recently gained much attention for biosensor development. Nanoshells, usually comprising a dielectric silica core enveloped in a highly conducting, ultrathin layer of silver or gold, constitute a new class of nanomaterials with tunable plasmon resonance, permitting materials to be particularly engineered to match the wavelength for specific applications, such as near infrared (NIR) areas where optimal light penetration through tissue is required [2,149]. Nanoshell substrates with surface enhanced Raman spectroscopy (SERS)-based sensors are promising platforms for in vivo detection [150,151,152].…”

“…The biosensor provided an exceptional response for real time O 2 •− sensing in cellular medium, offering a detection range of 0.8–1080 nM and a detection limit of 0.2 nM. Phan and co-workers used Cu shell -Au core nano particles for the determination of cultural filtrate protein (CFP-10), a Mycobacterium tuberculosis antigen, with the help of a dot-blot immunoassay, which allowed a highly sensitive detection by the naked eye [149]. Prior to growing the Cu nanoshells, gold binding polypeptide antibodies (GBPAb) were immobilised on the AuNPs.…”