Gold-based paper for antigen detection of monkeypox virus

Abstract

In 2022, the outbreak of the monkeypox virus occurred in many non-endemic countries, and the World Health Organization (WHO) assessed that this outbreak was "atypical". The establishment of a rapid...

Colloidal gold-based immunochromatographic biosensor for quantitative detection of S100B in serum samples

“…Hybridoma cells were screened with HAT selective medium, and after three rounds of subcloning, the hybridoma cell lines with the highest absorbance at 450 nm were selected. [26][27][28] Finally, the mAbs were obtained by protein G purification.…”

Gold nanoparticle-based immunochromatographic assay for the rapid detection of the SARS-CoV-2 Omicron variant

“…The protocol used for protein expression was similar to previous studies in our laboratory. 26,27 E. coli BL21 (DE3) lack Lon and OmpT proteases, which can effectively reduce the degradation of the target protein, and is the most commonly used strain for prokaryotic protein expression. First, the constructed plasmid pET28a-N was transformed into BL21 (DE3) competent cell and plated in the Luria-Bertani (LB) medium and incubated for 12-16 h at 37 1C.…”

Preparation of monoclonal antibodies against norovirus and establishment of a rapid immunochromatographic technique

“…The process of labeling HRP with purified antibodies was performed with reference to a previous article. 26 Antibodies labelled with HRP possess the ability of capturing antigens and catalyzing substrates. The specific pairing process was as follows.…”