Colloidal gold-based immunochromatographic biosensor for quantitative detection of S100B in serum samples

Abstract

Traumatic brain injury has become a serious public health problem. Timely detection, diagnosis and treatment of brain injury are closely related to the prognosis of patients, so identification of highly...

Establishment and application of a gold nanoparticle-based immunochromatographic test strip for the detection of avian leukosis virus P27 antigen in egg white samples

“…AuNPs are negatively charged and only in an alkaline environment can the positive charge on the protein band be stably combined with AuNPs. 33 Fig. 4(b) presents the UV-vis absorption spectrum of the AuNPs and AuNP-labeled mAbs.…”

Antibody-labeled gold nanoparticle based resonance Rayleigh scattering detection of S100B

“…To evaluate the selectivity of the proposed Ab-AuNP probe, we selected some interference in serum samples, such as S100A, NSE, GFAP and PGP9.5, which belong to the same family of calcium-binding proteins. 12…”

“…Existing S100B assays include enzyme-linked immunosorbent assay (ELISA), 6 magnetic bead quantum dot assay, 7 SPR sandwich photoelectrochemical immunoassay, 8 electrochemical assay, 9 paper lateral flow strips (PLFS), 10 peptide-modified ratiometric fluorescent nanoprobes, 11 and colloidal gold-based immunochromatographic biosensors. 12…”

Preparation of monoclonal antibodies against norovirus and establishment of a rapid immunochromatographic technique

“…The process of labeling HRP with purified antibodies was performed with reference to a previous article. 26 Antibodies labelled with HRP possess the ability of capturing antigens and catalyzing substrates. The specific pairing process was as follows.…”