Gold and Gold-Palladium Coated Polypropylene Grafts in a S. epidermidis Wound Infection Model

Saygun, Oral; Ağalar, Canan; Aydınuraz, Kuzey; Agalar, Fatih; Daphan, Çağatay Erden; Saygun, Meral; Çeken, Sabahat; Akkus, Abdullah; Denkbaş, Emir Baki

doi:10.1016/j.jss.2005.06.020

Cited by 41 publications

(30 citation statements)

References 33 publications

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“…Although the competing mechanisms remain unclear, a large body of data suggests that both physical and chemical modifications of a material surface can be engineered to limit bacterial colonization. [5][6][7][8][9][10]12,13,20,22 For example, as shown in Figure 1, coating surgical-grade titanium alloy with a synthetic PEM of PAA and PAH reduced colony density of waterborne S. epidermidis bacteria by orders of magnitude after immersion in 10 7 bacteria/mL. Reduced colonization over both 2 and 4 h incubation timescales is relevant to medical procedure durations involving, for example, cardiac assist and orthopedic implant devices.…”

Section: Bacterial Colonization Can Be Reduced By Materialsmentioning

confidence: 99%

Substrata Mechanical Stiffness Can Regulate Adhesion of Viable Bacteria

et al. 2008

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The competing mechanisms that regulate adhesion of bacteria to surfaces and subsequent biofilm formation remain unclear, though nearly all studies have focused on the role of physical and chemical properties of the material surface. Given the large monetary and health costs of medical-device colonization and hospital-acquired infections due to bacteria, there is considerable interest in better understanding of material properties that can limit bacterial adhesion and viability. Here we employ weak polyelectrolyte multilayer (PEM) thin films comprised of poly(allylamine) hydrochloride (PAH) and poly(acrylic acid) (PAA), assembled over a range of conditions, to explore the physicochemical and mechanical characteristics of material surfaces controlling adhesion of Staphylococcus epidermidis bacteria and subsequent colony growth. Although it is increasingly appreciated that eukaryotic cells possess subcellular structures and biomolecular pathways to sense and respond to local chemomechanical environments, much less is known about mechanoselective adhesion of prokaryotes such as these bacteria. We find that adhesion of viable S. epidermidis correlates positively with the stiffness of these polymeric substrata, independently of the roughness, interaction energy, and charge density of these materials. Quantitatively similar trends observed for wild-type and actin analogue mutant Escherichia coli suggest that these results are not confined to only specific bacterial strains, shapes, or cell envelope types. These results indicate the plausibility of mechanoselective adhesion mechanisms in prokaryotes and suggest that mechanical stiffness of substrata materials represents an additional parameter that can regulate adhesion of and subsequent colonization by viable bacteria.

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Section: Bacterial Colonization Can Be Reduced By Materialsmentioning

confidence: 99%

Substrata Mechanical Stiffness Can Regulate Adhesion of Viable Bacteria

et al. 2008

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“…After incubation with S. Epidermidis, they were laid under the musculoaponeurotic layer of inguinal region of rats. Gold-palladium coated polypropylene mesh showed the least bacterial growth in vitro and no signs of infection in vivo [117].…”

Section: Antibiotic Resistance and Promising Solutionsmentioning

confidence: 93%

Innovative Modifications for Preventing Mesh Infections

Aydınuraz¹

2017

J Microb Biochem Technol

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“…The control was a sterile piece of biomaterial on sterile TSB medium. Quantitative evaluation of biofilm formation was performed by a modified method used by Saygun et al [11]. Fragments of biomaterials, after 72-hour incubation in TSB medium with bacterial suspension of density of 0.5 as per MacFarland scale and in sterile TSB medium (control) were washed with PBS of pH 7.2 and shaken (1100 rpm) in 1 ml of 0.5% saponin solution (Fluka, Steinheim, Germany) for 2 minutes [13].…”

Section: Methodsmentioning

confidence: 99%

“…Evaluation of biofilm formation. The study was performed with the use qualitative method [9,10], quantitative method [11] and scanning electron microscopy (SEM) [12].…”

Section: Introductionmentioning

confidence: 99%

Biofilm formation on biomaterials used in hernia surgery

Reśliński

Dąbrowiecki

Głowacka

2014

MBS

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Head: Ryszard Górecki prof. S u m m a r yDeep surgical site infection (DSSI) in patients undergoing hernioplasty with implantation of biomaterials is a complication taking place with biofilm formation. Despite the indicated increase in the frequency of isolation of S. aureus and E. coli bacteria as etiological factors of DSSI, there have been few studies published so far that evaluated biofilm formation by these microorganisms on the surface of different biomaterials.The aim of this study was to evaluate and compare biofilm formation on the surface of biomaterials used in hernia surgery by clinical isolates of S. aureus and E. coli.70 strains of S. aureus and E. coli were used; they differed in chromosomal DNA within the species. The evaluation of biofilm formation on the surface of the monofilament polypropylene mesh, multifilament mesh (polypropylene, polyester and composite) and a patch of expanded polytetrafluoroethylene was made using qualitative and quantitative methods and by means of a scanning electron microscope.The strains differ in terms of biofilm formation within the species. Strains of S. aureus formed a biofilm more strongly than E. coli. The investigated strains formed biofilm stronger on the surface of the multifilament implants than on polypropylene monofilament mesh.Formation of biofilm by clinical isolates of S. aureus and E. coli on the surface of biomaterials used in hernia surgery varies depending on the strain and species of bacteria as well as the structure and the hydrophobicity of biomaterial. S t r e s z c z e n i e Głębokie zakażenie miejsca operowanego (GZMO) u pacjentów poddanych hernioplastyce z implantacją biomateriału jest powikłaniem przebiegającym z powstaniem biofilmu. Mimo, że notowany jest wzrost częstości izolacji bakterii S. aureus i E. coli jako czynników etiologicznych GZMO, dotychczas ukazały się nieliczne prace, w których oceniano tworzenie biofilmu przez te drobnoustroje na powierzchni różnych biomateriałów.Celem pracy była ocena i porównanie tworzenia biofilmu na powierzchni biomateriałów stosowanych w chirurgii przepuklin przez izolaty kliniczne S. aureus i E. coli.Użyto po 70 szczepów S. aureus i E. coli, izolowanych od różnych pacjentów hospitalizowanych w 3 klinikach chirurgii, różniących się wzorem DNA chromosomalnego w obrębie gatunku. Ocenę tworzenia biofilmu na powierzchni monofilamentowej siatki polipropylenowej, multifilamentowych siatek (polipropylenowej, poliestrowej i kompozytowej) oraz łacie z ekspandowanego politetrafluoroetylenu DOI: 10.2478/MBS.2014 wykonano metodą jakościową, ilościową oraz z użyciem skaningowego mikroskopu elektronowego.Szczepy różniły się zdolnością do tworzenia biofilmu w obrębie gatunku. Szczepy S. aureus tworzyły biofilm silniej niż pałeczki E. coli. Badane izolaty tworzyły biofilm silniej na powierzchni implantów multifilamentowych niż na monofilamentowej siatce polipropylenowej.Tworzenie biofilmu przez izolaty kliniczne S. aureus i E. coli na powierzchni biomateriałów stosowanych chirurgii przepuklin różni się w zależności ...

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Gold and Gold-Palladium Coated Polypropylene Grafts in a S. epidermidis Wound Infection Model

Cited by 41 publications

References 33 publications

Substrata Mechanical Stiffness Can Regulate Adhesion of Viable Bacteria

Substrata Mechanical Stiffness Can Regulate Adhesion of Viable Bacteria

Innovative Modifications for Preventing Mesh Infections

Biofilm formation on biomaterials used in hernia surgery

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