Substrata Mechanical Stiffness Can Regulate Adhesion of Viable Bacteria

Lichter, Jenny A.; Thompson, Michael T.; Delgadillo, Maricela; Nishikawa, Takehiro; Rubner, Michael F.; Vliet, Krystyn J. Van

doi:10.1021/bm701430y

Cited by 206 publications

(210 citation statements)

References 64 publications

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“…The plasma treatment has altered the mechanical properties of the top-most surface of the PDMS (Table 2). A positive correlation between substrate stiffness and the initial attachment of the bacteria Staphylococcus epidermis has been reported [46]. However this correlation does not apply in the case of the present study as lower bacteria attachment was observed for the plasma treated (harder) surface.…”

Section: Marine Bacteria: Attachment and Adhesion Strengthcontrasting

confidence: 58%

“…cally reduced when introducing fluorine into the PDMS coating. Bacterial adhesion to surfaces has been attributed to many factors, including surface chemical composition [42,43], surface hydrophilicity [44], surface roughness [45], and surface mechanical properties [46]. As no significant differences in surface roughness were detected between untreated and plasma treated samples (Figure 6), the differences observed in bacteria attachment cannot be attributed to differences in surface morphology.…”

Section: Marine Bacteria: Attachment and Adhesion Strengthmentioning

confidence: 99%

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Fluorination of poly(dimethylsiloxane) surfaces by low pressure CF4 plasma – physicochemical and antifouling properties

Cordeiro¹,

Nitschke²,

Janke³

et al. 2009

Express Polym. Lett.

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Abstract. Fluorinated surface groups were introduced into poly(dimethylsiloxane) (PDMS) coatings by plasma treatment using a low pressure radio frequency discharge operated with tetrafluoromethane. Substrates were placed in a remote position downstream the discharge. Discharge power and treatment time were tuned to alter the chemical composition of the plasma treated PDMS surface. The physicochemical properties and stability of the fluorine containing PDMS were characterized by X-ray photoelectron spectroscopy (XPS), atomic force microscopy (AFM) and contact angle measurements. Smooth PDMS coatings with a fluorine content up to 47% were attainable. The CF4 plasma treatment generated a harder, non-brittle layer at the top-most surface of the PDMS. No changes of surface morphology were observed upon one week incubation in aqueous media. Surprisingly, the PDMS surface was more hydrophilic after the introduction of fluorine. This may be explained by an increased exposure of oxygen containing moieties towards the surface upon re-orientation of fluorinated groups towards the bulk, and/or be a consequence of oxidation effects associated with the plasma treatment. Experiments with strains of marine bacteria with different surface energies, Cobetia marina and Marinobacter hydrocarbonoclasticus, showed a significant decrease of bacteria attachment upon fluorination of the PDMS surface. Altogether, the CF4 plasma treatments successfully introduced fluorinated groups into the PDMS, being a robust and versatile surface modification technology that may find application where a minimization of bacterial adhesion is required.

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Section: Marine Bacteria: Attachment and Adhesion Strengthcontrasting

confidence: 58%

Section: Marine Bacteria: Attachment and Adhesion Strengthmentioning

confidence: 99%

Fluorination of poly(dimethylsiloxane) surfaces by low pressure CF4 plasma – physicochemical and antifouling properties

Cordeiro¹,

Nitschke²,

Janke³

et al. 2009

Express Polym. Lett.

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“…18 Bacterial adhesion can also be affected by properties such as roughness, stiffness, and topography. 20,21 Reducing bacterial adhesion on the inner lumen of the ETT could reduce biofilm formation and perhaps prevent further colonization of the tube itself.…”

Section: Introductionmentioning

confidence: 99%

Decreased <em>Pseudomonas aeruginosa</em> biofilm formation on nanomodified endotracheal tubes: a dynamic lung model

Machado¹,

Webster²

2016

IJN

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Ventilator-associated pneumonia (VAP) is a serious complication of mechanical ventilation that has been shown to be associated with increased mortality rates and medical costs in the pediatric intensive care unit. Currently, there is no cost-effective solution to the problems posed by VAP. Endotracheal tubes (ETTs) that are resistant to bacterial colonization and that inhibit biofilm formation could provide a novel solution to the problems posed by VAP. The objective of this in vitro study was to evaluate differences in the growth of Pseudomonas aeruginosa on unmodified polyvinyl chloride (PVC) ETTs and on ETTs etched with a fungal lipase, Rhizopus arrhizus , to create nanoscale surface features. These differences were evaluated using an in vitro model of the pediatric airway to simulate a ventilated patient in the pediatric intensive care unit. Each experiment was run for 24 hours and was supported by computational models of the ETT. Dynamic conditions within the ETT had an impact on the location of bacterial growth within the tube. These conditions also quantitatively affected bacterial growth especially within the areas of tube curvature. Most importantly, experiments in the in vitro model revealed a 2.7 log reduction in the number (colony forming units/mL) of P. aeruginosa on the nanoroughened ETTs compared to the untreated PVC ETTs after 24 hours. This reduction in total colony forming units/mL along the x -axis of the tube was similar to previous studies completed for Staphylococcus aureus . Thus, this dynamic study showed that lipase etching can create surface features of nanoscale roughness on PVC ETTs that decrease bacterial attachment of P. aeruginosa without the use of antibiotics and may provide clinicians with an effective and inexpensive tool to combat VAP.

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“…The type, concentration, conformation, and bioactivity of proteins adsorbed onto a material depend on its topographical (roughness), chemical, physical (charge and hydrophilicity), and mechanical (stiffness) properties, all of which can be easily influenced by nanotechnology (2). Surface properties, including topography, also effect bacterial adhesion and may play an important role in the initial stages of biofilm formation (3,4).…”

mentioning

confidence: 99%

Nanotechnology: Pediatric Applications

et al. 2010

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Ventilator-associated pneumonia (VAP) is a serious and costly clinical problem affecting pediatrics today. This devicerelated infection is thought to be directly linked to the colonization of the endotracheal tube (ETT) during long-term mechanical ventilation. Because of unspecific radiographic and clinical signs, VAP is especially difficult to diagnose in the pediatric population. Treatment with antibiotics is often ineffective, and VAP is associated with high morbidity, mortality, and medical costs. The use of nanomodified coatings on ETT may provide an effective strategy to prevent biofilm formation and ETT colonization. Nanoparticles such as selenium and iron oxide have been shown to penetrate into the biofilm reaching the protected cells antibiotics often miss. Moreover, nanoetching techniques can modify the topography of the ETT surface interfering with bacterial adhesion. This review seeks to examine the antimicrobial properties of both nanoparticles and nanomodified surfaces and to characterize their effectiveness at reducing bacterial colonization on ETT. (Pediatr Res 67: 500-504, 2010) N anotechnology is the control of matter at the atomic, molecular, and supramolecular scale. Nanomaterials, or materials (such as particles, fibers, tubes, grains, etc.) with at least one dimension in the range of 1-100 nm, can be metals, ceramics, polymers, or composites thereof. These materials exhibit unique properties because of their size and significantly greater surface areas, which can influence numerous properties including material conductivity, magnetic properties, surface energy, mechanical properties, catalytic properties, etc.An ongoing area of this research includes the interaction between synthetic nanoscale materials and living tissues. Micro-and nanoscale building blocks form the foundation for cells and tissues within the human body. It is thought that the difference in the activity of some cells on nanomodified surfaces is because of the ability of these materials to mimic the natural dimensions of constituents of biological tissues. Another important factor in this interaction is the unique surface energetics of nanomaterials because of their significantly greater surface areas compared with conventional, micron-structured materials. Such changes in surface energy undoubtedly influence initial protein interactions that are important for mediating bacteria and nonbacteria cell adhesion. Specifically, one of the first steps within the process of cell adhesion is the association of proteins adsorbed on implant surfaces to cell membrane receptors. The large surface to volume ratio characteristic of nanomaterials has been shown to affect this association to inhibit bacteria attachment and promote nonbacterial cell (such as osteoblasts, smooth muscle cells, endothelial cells, chondrocytes, etc.) adhesion (1). The type, concentration, conformation, and bioactivity of proteins adsorbed onto a material depend on its topographical (roughness), chemical, physical (charge and hydrophilicity), and mecha...

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Substrata Mechanical Stiffness Can Regulate Adhesion of Viable Bacteria

Cited by 206 publications

References 64 publications

Fluorination of poly(dimethylsiloxane) surfaces by low pressure CF4 plasma – physicochemical and antifouling properties

Fluorination of poly(dimethylsiloxane) surfaces by low pressure CF4 plasma – physicochemical and antifouling properties

Decreased <em>Pseudomonas aeruginosa</em> biofilm formation on nanomodified endotracheal tubes: a dynamic lung model

Nanotechnology: Pediatric Applications

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