Goat oocyte quality and competence to undergo IVM and embryo development after parthenogenetic activation from goats fed with different levels of cashew nut bran as source of dietary lipids

Fernandes, César Carneiro Linhares; Feltrin, Cristiano; Martins, Leonardo Tondello; Neto, S. Gaudencio; Aguiar, L. H.; Silva, A.M.; Oliveira, C.H.A.; Silva, L.M.; Silva, C.M.G.; Bertolini, Marcelo; Rondina, Davide

doi:10.1016/j.theriogenology.2014.04.011

Cited by 9 publications

(5 citation statements)

References 28 publications

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“…In this study, we verified that the glucose rate of animals fed glycerine was higher than that of the control group, which corroborates previous researches that concluded that glycerine use improves the energy balance in ruminants (Chung et al, ). Likewise, cholesterol levels followed the increase in diet lipid levels, similar to that observed in the study carried out by Fernandes et al (). Also, Alves et al () used a diet supplemented with 12% dry matter‐based lipids and found mean values of plasma cholesterol close to those observed in the present experiment (74.7 mg/dl), which corroborate with the reference values established by Cozna et al ().…”

Section: Discussionsupporting

confidence: 88%

“…Nutritional strategies have been proposed to improve follicular dynamics seeking to increase efficiency in production systems and results in small ruminants (Useni & Cruywagen, ), which can be mediated by multiple mechanisms, including interactions between metabolite concentrations and reproductive hormones (Scaramuzzi et al, ). Several studies have shown that glycerol or lipid‐based nutritional stimuli are capable of promoting changes in plasma metabolites such as glucose (Silva et al, ), insulin (Rodrigues et al, ) and cholesterol (Alves et al, ; Fernandes et al, ). In the case of crude glycerine, composed of 80%–88% glycerol, there is a rapid generation of propionic acid, considered a trigger of pancreatic insulin secretion (Donkin, ), whereas lipids are considered excellent energy sources because they are composed in significant proportion by fatty acids, which have a much higher energy potential than carbohydrates.…”

Section: Discussionmentioning

confidence: 99%

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Impact of short nutrient stimuli with different energy source on follicle dynamics and quality of oocyte from hormonally stimulated goats

Alves

Fernandes

Rossetto

et al. 2019

Reprod Domestic Animals

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The aim of this study was to verify the effect of the energy source for a short‐term diet supplementation on follicular dynamics, ovarian response and oocyte recovery in goats. Thirty Anglo Nubian crossbred does received a diet for 4 weeks to satisfy the nutritional requirements of breeding for adult non‐dairy goats. Seven days prior to oocyte recovery (OR), a group of does (n = 10) was supplemented with ground full‐fat linseed in the diet (Diet A), whereas a second group of does (n = 10) received crude glycerine in the diet (Diet B). The total mixed ration (TMR) diet was maintained as the Control Diet (n = 10). All animals were oestrous‐synchronized by the use of a progesterone insert for 12 days prior to OR. Follicles were stimulated by using pFSH (five 40‐mg/ml doses) during the supplementation time. At OR, follicles were counted and recovered oocytes were classified as viable or degenerated. Follicular dynamics was monitored by ultrasonography, and plasma glucose, cholesterol and triglyceride levels were measured during supplementation. Glucose was higher in Diet B and cholesterol in Diet A. Diet B had a lower proportion of small (<3 mm) and large follicles (≥3 mm; p = 0.01). The follicular growth rate was higher in Diet A (p < 0.01), with follicles emerging in the 5th day of supplementation. No differences were observed for follicles counted and oocytes recovered. Thus, the type of energy source supplemented for a short term was capable to alter the follicular dynamics, without affecting the proportion of morphologically viable oocytes upon recovery.

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Section: Discussionsupporting

confidence: 88%

Section: Discussionmentioning

confidence: 99%

Impact of short nutrient stimuli with different energy source on follicle dynamics and quality of oocyte from hormonally stimulated goats

Alves

Fernandes

Rossetto

et al. 2019

Reprod Domestic Animals

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“…However, high feeding levels after ovulation suppress progesterone levels in the blood and change the constituents of the uterine fluid, affecting embryonic survival (Jalali, Likszo, & Skarzynski, ; Koch et al, ). In addition, studies have been conducted about the effect of feeding high levels of energy or protein on follicular activity and oocyte quality (Fernandes et al, ), but there are no investigation about the effects of nutrition on the oviduct and uterus capacity to establish and maintain pregnancy in goats.…”

Section: Introductionmentioning

confidence: 99%

Proteome of the periovulatory oviduct and uterus of goats as related to nutritional balance

Fernandes

Rodríguez-Villamil

Vasconcelos

et al. 2018

Reprod Domestic Animals

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This study was conducted to evaluate the effects of different feeding levels on the proteome of oviduct and uterus tissues of hormonally stimulated goats during the periovulatory period. Forty goats were separated into four different diet groups: Diet 1.0 M (n = 11), Diet 1.3 M (n = 10), Diet 1.6 M (n = 9), Diet 1.9 M (n = 10), fed with 1.0, 1.3, 1.6 and 1.9 times live weight maintenance, respectively. After four weeks of treatment, six hormonally stimulated females per treatment group were randomly selected for collection of uterine and the oviduct tissue samples. Samples were collected after animals were slaughtered in a commercial unit. Feeding goats with 1.3 to 1.9 times more nutrients than a control group directly influenced the proteome of the oviduct and uterus, altering the expression of proteins that participate in biological processes such as apoptosis, antioxidant, and immunological activities. These events are crucial for fertilization and early embryonic survival. Expression of oviduct proteins such as Tubulin Beta 2B, Transferrin and Disulphide-isomerase A3 increased in the 1.9 M group in relation to the other feeding levels. Disulphide-isomerase A4 showed higher expression in the 1.0 M group compared to diets with higher energetic levels. As energy intake increased in the diets, there was higher expression of Alpha-1-antitrypsin and downregulation of Profilin-1 in the uterus of the goats. In conclusion, this study showed that specific proteins of the goat oviduct and uterus expressed during the periovulatory period are modified as the result of nutritional balance.

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“…Selected COCs, segregated as viable by grade (G-I to G-III) according to morphological quality and experimental groups, were subjected to IVM, parthenogenetic activation and IVC procedures according to Fernandes et al (2014) , with minor modifications. Briefly, COCs were subjected to IVM at 38.5°C and 5% CO 2 in petri dishes (Corning ® , USA) under mineral oil-containing drops of 100 µL of maturation medium.…”

Section: Methodsmentioning

confidence: 99%

Effectiveness of near-infrared spectroscopy as a non-invasive tool to discriminate spectral profiles of in vitro cultured oocytes from goats

et al. 2021

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Here, we aimed to discriminate between the spectral profiles of spent culture media after oocyte in vitro maturation (IVM) and culture (IVC) from goats of different ages subjected to repeated hormonal treatments. The profiles were discriminated using near infrared (NIR) spectroscopy combined with multivariate methods. A total of 19 goats (young = 10; old = 9) were subjected to serial hormonal stimulation (HS) with gonadotropins. Cumulus oophorus complexes (COCs) were collected using laparoscopic ovum pick-up (LOPU) and subjected to IVM and parthenogenetic activation. The initial embryos were subjected to IVC. Spent culture media were collected after oocyte IVM and on day 2 of IVC and analyzed using NIR spectroscopy. NIR spectral data were interpreted through chemometric methods, such as principle component analysis (PCA) and partial least square discriminant analysis (PLS-DA). The results of PCA analysis clearly showed a separation in the spectral profiles between the experimental groups (HS sessions; young and old animals) both after IVM and IVC. Overall, the main absorption bands were attributed to the C-H group second overtone, first overtone of O-H and N-H, and C-H combinations and may serve as molecular markers. On the other hand, the spectral data obtained using PLS-DA models provided a better classification of the groups. The results showed the possibility of discriminating young and old groups as well as the three HS sessions with high specificity, sensitivity, and accuracy using NIR spectra. Thus, the culture medium analysis using NIR spectroscopy combined with multivariate methods indicated the dissimilarities between the groups and provided an insight into the in vitro development of goat oocytes. This technique serves as an efficient, objective, rapid, and non-invasive method to discriminate spectral profiles.

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Goat oocyte quality and competence to undergo IVM and embryo development after parthenogenetic activation from goats fed with different levels of cashew nut bran as source of dietary lipids

Cited by 9 publications

References 28 publications

Impact of short nutrient stimuli with different energy source on follicle dynamics and quality of oocyte from hormonally stimulated goats

Impact of short nutrient stimuli with different energy source on follicle dynamics and quality of oocyte from hormonally stimulated goats

Proteome of the periovulatory oviduct and uterus of goats as related to nutritional balance

Effectiveness of near-infrared spectroscopy as a non-invasive tool to discriminate spectral profiles of in vitro cultured oocytes from goats

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