GnRH localization in the equine brain and infundibulum: An immunohistochemical study

Dees, W. Les; Sorensen, A. M.; Kemp, Walter M.; McArthur, N. H.

doi:10.1016/0006-8993(81)90625-9

Cited by 15 publications

(9 citation statements)

References 29 publications

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“…Our findings that there is a greater concentration of KiSS-ir cells in the ARC rather than the POA are also qualitatively similar to the distribution of kisspeptin-containing neurons that has been reported in sheep (19,54), monkeys (10,55), and humans (55). This is also consistent with our knowledge that whereas the anteroventral paraventricular nucleus is thought of as a GnRH/LH surge center in the rodent (56), it appears to be lacking in the human (57), mare (58,59), and ewe (60). Although more study will be necessary, this body of evidence suggests that kisspeptin in the equine may be involved in both positive and negative feedback for hypothalamic GnRH output via the arcuate nucleus as has been proposed for higher mammals (19).…”

Section: Discussionsupporting

confidence: 91%

Biological and Anatomical Evidence for Kisspeptin Regulation of the Hypothalamic-Pituitary-Gonadal Axis of Estrous Horse Mares

Magee¹,

Foradori

Bruemmer

et al. 2009

Endocrinology

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The purpose of the present study was to evaluate the effects of kisspeptin (KiSS) on LH and FSH secretion in the seasonally estrous mare and to examine the distribution and connectivity of GnRH and KiSS neurons in the equine preoptic area (POA) and hypothalamus. The diestrous mare has a threshold serum gonadotropin response to iv rodent KiSS decapeptide (rKP-10) administration between 1.0 and 500 microg. Administration of 500 microg and 1.0 mg rKP-10 elicited peak, mean, and area under the curve LH and FSH responses indistinguishable to that of 25 microg GnRH iv, although a single iv injection of 1.0 mg rKP-10 was insufficient to induce ovulation in the estrous mare. GnRH and KiSS-immunoreactive (ir) cells were identified in the POA and hypothalamus of the diestrous mare. In addition, KiSS-ir fibers were identified in close association with 33.7% of GnRH-ir soma, suggesting a direct action of KiSS on GnRH neurons in the mare. In conclusion, we are the first to reveal a physiological role for KiSS in the diestrous mare with direct anatomic evidence by demonstrating a threshold-like gonadotropin response to KiSS administration and characterizing KiSS and GnRH-ir in the POA and hypothalamus of the diestrous horse mare.

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Section: Discussionsupporting

confidence: 91%

Biological and Anatomical Evidence for Kisspeptin Regulation of the Hypothalamic-Pituitary-Gonadal Axis of Estrous Horse Mares

Magee¹,

Foradori

Bruemmer

et al. 2009

Endocrinology

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“…A recent study which utilized the same antiserum for immunocytochemical procedures in the rat also failed to visualize reactive perikarya [17]. However, immunoreactive LHRH containing perikarya have been localized with this antiserum in the horse [11], sheep [12], and cow [10].…”

Section: Discussionmentioning

confidence: 89%

Immunocytochemical Evidence that Suckling Inhibits the Postovariectomy Depletion of Median Eminence Luteinizing Hormone Releasing Hormone

Culler¹,

McArthur²,

Dees³

et al. 1982

Neuroendocrinology

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Suckling has been demonstrated to impair the release of pituitary luteinizing hormone (LH) and to prevent the dramatic increase in plasma LH observed following ovariectomy. In the present study, the effect of suckling (10 pups/animal) for either 1 or 3 weeks on the relative amount of luteinizing hormone releasing hormone (LHRH) present in the hypothalamus and preoptic area of ovariectomized and intact rats was examined using immunocytochemical methodology. Controls consisted of nonlactating animals which were either intact (diestrous) or ovariectomized for 1 or 3 weeks. Brains were removed following transcardial perfusion of phosphate-buffered formalin and Bouin’s fixative. After dehydration, clearing and paraffin embedding, the brains were sectioned and LHRH localized by an indirect immunoperoxidase technique. A positive reaction denoting the presence of immunoreactive LHRH was observed over axons and termini throughout the rostral to caudal extent of the median eminence (ME) and surrounding the organum vasculosum of the lamina terminalis (OVLT) in the preoptic area. Ovariectomy resulted in a progressive decline in the concentration of LHRH within the ME as evidenced by a reduction in the intensity of the staining reaction and in the number of axons over which the reaction was observed. In contrast, brains from ovariectomized rats which had been suckled appeared to have concentrations of LHRH in the ME equal to or greater than that of the diestrous controls. Similarly, the concentration of LHRH In the ME of intact, suckled rats did not differ significantly from that of the diestrous controls. Neither ovariectomy nor suckling produced any observable change in the relative concentration of LHRH located near the OVLT. These data demonstrate that suckling prevents the depletion of LHRH from the ME following ovariectomy and provide evidence for mechanism by which the suckling stimulus may suppress plasma LH.

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“…Kelch) used at 1:700 to 1:1,000 dilutions for 24 to 48 hours at 4°C. Specificity of this antiserum for use in immunohistochemistry has been reported (Gross, 1976;Gross and Baker, 1977;Dees et al, 1981b). This was followed by sheep anti-rabbit globulin serum (Miles) used at a 1:200 dilution for 30 minutes at room temperature, rabbit PAP complex (Miles) used at a 1:200 dilution for 30 minutes at room temperature and 0.05% 3.3' diaminobenzidine (Sigma) with 0.003% hydrogen peroxide for 5 to 10 minutes at room temperature on an automatic shaker.…”

Section: Zmmunohistochemical Techniquesmentioning

confidence: 94%

“…Hoffman et al, 1978;G. Hoffman et al, 1978;Dees et al, 1981a;Polkowska et al, 1980) and the horse (Dees et al, 1981b). To date, the distribution of GnRH within the brain and infundibulum (INF) of the cow has not been reported.…”

mentioning

confidence: 99%

“…To date, the distribution of GnRH within the brain and infundibulum (INF) of the cow has not been reported. Reports indicate species differences in precise GnRH localization in the INF of a variety of animals (Baker et al, 1974;Dees et al, 1981b). The guinea pig (Silverman, 1976) and the rhesus monkey (Silverman et al, 1977) appear to contain a wider hypothalamic and extrahypothalamic distribution of the peptide than other animals.…”

mentioning

confidence: 99%

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Immunoshistochemical localization of gonadortropin releasing hormone (GnRH) in the bovine hypothalamus and infundibulum

Dees

McArthur

1981

Anat. Rec.

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Neural structures containing gonadotropin-releasing hormone (GnRH) in the bovine brain and infundibulum were characterized by light microscopic immunohistochemistry. GnRH-positive perikarya were localized singly or in small groups within the infundibular nucleus and were seen grouped in large discrete clusters within the ventromedial nucleus. Positive axons were localized within the brain from the nucleus of the rostral commissure to the medial mammillary nuclei, and in specific areas of the infundibulum and infundibular stalk.

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GnRH localization in the equine brain and infundibulum: An immunohistochemical study

Cited by 15 publications

References 29 publications

Biological and Anatomical Evidence for Kisspeptin Regulation of the Hypothalamic-Pituitary-Gonadal Axis of Estrous Horse Mares

Biological and Anatomical Evidence for Kisspeptin Regulation of the Hypothalamic-Pituitary-Gonadal Axis of Estrous Horse Mares

Immunocytochemical Evidence that Suckling Inhibits the Postovariectomy Depletion of Median Eminence Luteinizing Hormone Releasing Hormone

Immunoshistochemical localization of gonadortropin releasing hormone (GnRH) in the bovine hypothalamus and infundibulum

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