GmNAP1 is essential for trichome and leaf epidermal cell development in soybean

Abstract

Key message Map-based cloning revealed that two novel soybean distorted trichome mutants were due to loss function of GmNAP1 gene, which affected the trichome morphology and pavement cell ploidy by regulating actin filament assembly. Abstract Trichomes increase both biotic and abiotic stress resistance in soybean. In this study, Gmdtm1-1 and Gmdtm1-2 mutants with shorter trichomes and bigger epidermal pavement cells were isolated from an ethyl methylsulfonate mutagenized population. Both of them had reduced plant height and smaller seeds. Map-based cloning and bulked segregant analysis identified that a G-A transition at the 3ʹ boundary of the sixth intron of Glyma.20G019300 in the Gmdtm1-1 mutant and another G-A transition mutation at the 5ʹ boundary of the fourteenth intron of Glyma.20G019300 in Gmdtm1-2; these mutations disrupted spliceosome recognition sites creating truncated proteins. Glyma.20G019300 encodes a Glycine max NCK-associated protein 1 homolog (GmNAP1) in soybean. Further analysis revealed that the GmNAP1 involved in actin filament assembling and genetic information processing pathways during trichome and pavement cell development. This study shows that GmNAP1 plays an important role in soybean growth and development and agronomic traits.

A Pd1–Ps–P1 Feedback Loop Controls Pubescence Density in Soybean

“…Recently, it was found that P2, an NCK-associated protein 1 (NAP1) encoding gene, and the homologs of Arabidopsis CPR5 play essential roles in soybean trichome development. Loss of function of these two genes results in abnormal trichome growth (Campbell et al 2016(Campbell et al , 2019Tang et al, 2020). In this study, we focused on the genetics that underlies pubescence density and cloned the genes responsible for the classic loci Pd1, Ps, and P1.…”

“…The genes GmCPR5 and GmNAP1 were reported to be involved in pubescence development (Campbell et al 2016(Campbell et al , 2019Tang et al, 2020). To determine whether Pd1, Ps, and P1 affect GmCPR5 and GmNAP1, we analyzed the transcriptional levels of GmCPR5 and GmNAP1 in DN50 and the Pd1, Ps, and P1 transgenic lines.…”

Advances in the Regulation of Epidermal Cell Development by C2H2 Zinc Finger Proteins in Plants

“…Cell differentiation and morphogenesis must occur at the correct time and place to ensure the normal growth and development of multicellular organisms (Song et al, 2019;Haas et al, 2020;Su et al, 2021). Plant cell differentiation is precisely and intricately regulated to enable these organisms to successfully complete their lifecycles (Glover, 2000;Tang et al, 2020). Root hairs, stomata, trichomes, and salt glands, the major epidermal structures of terrestrial plants, play essential roles in plant growth, development, and adaptation to environmental stress (Yuan et al, 2019b;Li et al, 2021).…”

GmPGL2, Encoding a Pentatricopeptide Repeat Protein, Is Essential for Chloroplast RNA Editing and Biogenesis in Soybean

“…The homologs of GmPGL2 protein were characterized by BLAST tool in Phytozome 1 ; the phylogenetic and microsyntynteny analysis were performed as described previously ( Tang et al, 2020 ). The signal peptide of GmPGL2 protein was analyzed by TargetP2.0 program.…”

“…All samples had at least three biological replicates. The statistical analyses were performed using R software (version 3.6.2) as described previously ( Tang et al, 2020 ). Asterisks indicate significant differences as determined by p values ( * p <0.05; * p <0.01; and ** p <0.001).…”