A Pd1–Ps–P1 Feedback Loop Controls Pubescence Density in Soybean

Tang

et al. 2021

Preprint

Leaf trichomes play vital roles in plant resistance and tea quality. Basic helix-loop-helix (bHLH) transcription factors (TFs) play an important role in regulating plant development and growth. However, it is poorly understood whether bHLH TFs are associated with trichome formation in tea plant. In this study, a total of 134 CsbHLH proteins were identified in the Camellia sinensis var. sinensis (CSS) genome. All identified proteins were divided into 19 subgroups according to the Arabidopsis thaliana classification. Phylogenetic tree analysis indicated that the members of group IIIc-I and group IIIc-II might be associated with trichome formation. Expression analysis showed that the candidate genes associated with trichome formation in tea plant were primarily located in cluster 1, cluster 3 and cluster 5. The expression patterns of CsbHLH116, CsbHLH133, CsbHLH060, CsbHLH028, CsbHLH024, CsbHLH112 and CsbHLH053 from these clusters were similar to the trichome distribution in tea plants. Notably, CsbHLH024 and CsbHLH133 were highly expressed in the young tissues of different cultivars. CsbHLH024 and CsbHLH133 possessed transcriptional activation ability and could interact with CsTTG1, a regulator of tea trichome formation. This study provides useful information for further research on the function of CsbHLHs in the regulation of trichome formation.

Section: Phylogenetic Tree Analysis Of Csbhlh Proteinsmentioning

confidence: 99%

“…Trichome formation has been systematically investigated in Arabidopsis thaliana, Solanum lycopersicum, Cucumis sativus L., Oryza sativa L., Nicotiana tabacum L., Gossypium spp. and Glycine max [15][16][17][18][19][20][21]. Additionally, trichome formation is induced by cell differentiation.…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Identification of Tea Plant bHLH Transcription Factor Family and Discovery of Candidate Regulator for Trichome Formation

Liu

Tang

et al. 2021

Preprint

“…Other genes that affect drought tolerance in soybean have also been identified. For instance, the genes responsible for pubescence density, Pd1 , P1 , Ps and Pd2 , were also found to be involved in drought tolerance in soybean (Liu et al ., 2020c; Pfeiffer et al ., 2003). Overexpression of the LOS5/ABA3 gene, which encodes a molybdenum cofactor sulfurase, using a constitutive expression promoter in soybean enhanced drought tolerance and increased seed yield by at least 21% under drought stress (Li et al ., 2013a).…”

Section: Genes Responsible For Important Agronomic Traitsmentioning

confidence: 99%

Progress in soybean functional genomics over the past decade

Zhang

Liu

Plant Biotechnology Journal

et al. 2021

Self Cite

Summary Soybean is one of the most important oilseed and fodder crops. Benefiting from the efforts of soybean breeders and the development of breeding technology, large number of germplasm has been generated over the last 100 years. Nevertheless, soybean breeding needs to be accelerated to meet the needs of a growing world population, to promote sustainable agriculture and to address future environmental changes. The acceleration is highly reliant on the discoveries in gene functional studies. The release of the reference soybean genome in 2010 has significantly facilitated the advance in soybean functional genomics. Here, we review the research progress in soybean omics (genomics, transcriptomics, epigenomics and proteomics), germplasm development (germplasm resources and databases), gene discovery (genes that are responsible for important soybean traits including yield, flowering and maturity, seed quality, stress resistance, nodulation and domestication) and transformation technology during the past decade. At the end, we also briefly discuss current challenges and future directions.

“…Both non-glandular trichomes (providing physical defense) and glandular trichomes (providing chemical defense) serve as defenses against herbivorous insects. Many phytohormones are involved in trichome development, such as salicylic acid (SA), jasmonic acid (JA), gibberellins (GAs), abscisic acid (ABA), brassinosteroids (BRs), auxin and cytokinin (CK) ( Zhou et al, 2013 ; Yan et al, 2018 ; Liu S. et al, 2020 ; Tan et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Phytohormone-Based Regulation of Trichome Development

Jiang

et al. 2021

Front. Plant Sci.

Phytohormones affect plant growth and development. Many phytohormones are involved in the initiation of trichome development, which can help prevent damage from UV radiation and insect bites and produce fragrance, flavors, and compounds used as pharmaceuticals. Phytohormones promote the participation of transcription factors in the initiation of trichome development; for example, the transcription factors HDZIP, bHLH and MYB interact and form transcriptional complexes to regulate trichome development. Jasmonic acid (JA) mediates the progression of the endoreduplication cycle to increase the number of multicellular trichomes or trichome size. Moreover, there is crosstalk between phytohormones, and some phytohormones interact with each other to affect trichome development. Several new techniques, such as the CRISPR-Cas9 system and single-cell transcriptomics, are available for investigating gene function, determining the trajectory of individual trichome cells and elucidating the regulatory network underlying trichome cell lineages. This review discusses recent advances in the modulation of trichome development by phytohormones, emphasizes the differences and similarities between phytohormones initially present in trichomes and provides suggestions for future research.