GmKIX8‐1 regulates organ size in soybean and is the causative gene for the major seed weight QTL <i>qSw17‐1</i>

Nguyen, Cuong Xuan; Paddock, Kyle J; Zhang, Zhanyuan; Stacey, Minviluz G.

doi:10.1111/nph.16928

Cited by 67 publications

(87 citation statements)

References 84 publications

(149 reference statements)

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“…The identified stable QTLs will pave the way for positional cloning of genes regulating seed size and shape traits in soybean. Several QTLs and genes controlling seed size traits have been cloned in other crop species, but similar studies are lagging in soybean (Mao et al, 2010;Li et al, 2011;Ma et al, 2015;Xu et al, 2015;Lu et al, 2017;Nguyen et al, 2020). The physical interval of the qSS2, spans ∼887 Kb and harbors 110 protein coding genes.…”

Section: Discussionmentioning

confidence: 99%

A Major and Stable Quantitative Trait Locus qSS2 for Seed Size and Shape Traits in a Soybean RIL Population

Kumawat

2021

Front. Genet.

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Seed size and shape traits are important determinants of seed yield and appearance quality in soybean [Glycine max (L.) Merr.]. Understanding the genetic architecture of these traits is important to enable their genetic improvement through efficient and targeted selection in soybean breeding, and for the identification of underlying causal genes. To map seed size and shape traits in soybean, a recombinant inbred line (RIL) population developed from K099 (small seed size) × Fendou 16 (large seed size), was phenotyped in three growing seasons. A genetic map of the RIL population was developed using 1,485 genotyping by random amplicon sequencing-direct (GRAS-Di) and 177 SSR markers. Quantitative trait locus (QTL) mapping was conducted by inclusive composite interval mapping. As a result, 53 significant QTLs for seed size traits and 27 significant QTLs for seed shape traits were identified. Six of these QTLs (qSW8.1, qSW16.1, qSLW2.1, qSLT2.1, qSWT1.2, and qSWT4.3) were identified with LOD scores of 3.80–14.0 and R2 of 2.36%–39.49% in at least two growing seasons. Among the above significant QTLs, 24 QTLs were grouped into 11 QTL clusters, such as, three major QTLs (qSL2.3, qSLW2.1, and qSLT2.1) were clustered into a major QTL on Chr.02, named as qSS2. The effect of qSS2 was validated in a pair of near isogenic lines, and its candidate genes (Glyma.02G269400, Glyma.02G272100, Glyma.02G274900, Glyma.02G277200, and Glyma.02G277600) were mined. The results of this study will assist in the breeding programs aiming at improvement of seed size and shape traits in soybean.

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Section: Discussionmentioning

confidence: 99%

A Major and Stable Quantitative Trait Locus qSS2 for Seed Size and Shape Traits in a Soybean RIL Population

Kumawat

2021

Front. Genet.

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“…Production of enlarged leaves with uneven lamina is also observed upon down-regulation of PPD or KIX and up-regulation of SAP orthologues in Medicago truncatula (medicago; BIG SEEDS 1, mtbs1-1, SMALL LEAF AND BUSHY 1 (SLB1)) [60,61], Vigna mungo (black gram; MULTIPLE ORGAN GIGANTISM (MOG), VmPPD) [62], Pisum sativum (pea; ELEPHANT-EAR-LIKE LEAF 1 (ELE1), PsPPD, BIGGER ORGANS (BIO), PsKIX) [63], Glycine max (soybean; BIG SEEDS 1/2 (BS1/2), GmPPD1/2, GmKIX8-1) [60,62,64,65], Capsella rubella (CrSAP) [66], Cucumis sativus L. (cucumber; LITTLELEAF (LL), CsSAP) [67] and Populus tremula x P. alba (P. x canescens, grey poplar; BIG LEAF (BL), SAP orthologue) [68], suggesting a highly conserved role for the PPD pathway in leaf growth regulation across rosid eudicot species (Table 1, Figure 3). More recently, also Solanum lycopersicum (tomato) Slkix8-kix9 plants were shown to produce enlarged and rippled leaves, demonstrating that the pathway is also conserved in asterid eudicots (Table 1, Figure 3) [69].…”

Section: Leaf Developmentmentioning

confidence: 99%

“…In multiple eudicot species, down-regulation of PPD or KIX or up-regulation of SAP orthologues also significantly affects other plant organs (Table 1, Figure 3) [42,46,47,51,60,62,64,65,68,83].…”

Section: Flower Fruit and Seed Developmentmentioning

confidence: 99%

“…Besides fruit size, disruption or overexpression of PPD1/2, KIX8/9 or SAP also affects seed size and weight in arabidopsis [42,46,47,83], as well as in medicago (BS1, mtbs1-1, SLB1) [60,61], black gram (MOG, VmPPD) [62], pea (ELE1, PsPPD; BIO, PsKIX) [63], soybean (BS1, GmPPD1/2; GmKIX8-1/-2) [60,62,64,65] and cucumber (LL, CsSAP) [67] (Table 1, Figure 3). Whereas also soybean seed quality, more specifically the amino acid content, is significantly improved in bs1 seeds compared with the wild type [60], the increase in seed size comes at the expense of seed number in black gram [62] and soybean [62,64].…”

Section: Flower Fruit and Seed Developmentmentioning

confidence: 99%

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The PEAPOD Pathway and Its Potential To Improve Crop Yield

Schneider

González

Pauwels

et al. 2021

Trends in Plant Science

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A key strategy to increase plant productivity is to improve intrinsic organ growth. Some of the regulatory networks underlying organ growth and development, and interconnections between these networks are highly conserved. An example of such a growth-regulatory module with a highly conserved role in final organ size and shape determination in eudicot species is the PEAPOD (PPD)/KINASE-INDUCIBLE DOMAIN INTERACTING (KIX)/STERILE APETALA (SAP) module. Here, we review the proteins constituting the PPD pathway and their role in different plant developmental processes and explore options for future research. Moreover, we speculate on strategies to exploit the knowledge of the PPD pathway for targeted yield improvement to engineer crop traits of agronomical interest, such as leaf, fruit and seed size. JAZ and PPD Proteins: So Similar, Yet So Different Various regulators of organ size, shape and differentiation, their targets, interacting proteins and the interconnections amongst them have been described [1-6]. One such pathway with highly conserved functions for the development of distinct plant organs in various eudicot species is the PEAPOD (PPD) pathway. PPD1 and PPD2 are transcriptional regulators that, together with the JASMONATE ZIM-DOMAIN (JAZ) proteins and TIFY DOMAIN PROTEIN 8 (TIFY8), constitute the plant-specific class II TIFY protein family [7-11]. JAZ proteins contain a ZINC-FINGER PROTEIN EXPRESSED IN INFLORESCENCE MERISTEM (ZIM) domain, containing a core TIF[F/Y]XG motif and a jasmonic acid (JA)-associated (Jas) domain, and are well-described as negative regulators of JA signalling (Figure 1A) [9,12,13]. In the absence of JA, they bind and repress multiple transcriptional regulators, including MYC and R2R3 MYB transcription factors (Figure 1A) [9,14-19]. To function as transcriptional repressors, JAZ proteins act as adaptors to recruit the corepressor TOPLESS (TPL) [9,20-22], which is, together with TOPLESS-RELATED (TPR) proteins, involved in several processes, such as meristem maintenance, hormone signalling and the control of flowering time [22-24]. Whereas JAZ5 to JAZ8 in Arabidopsis thaliana (arabidopsis) contain an ETHYLENE RESPONSE FACTOR (ERF)-ASSOCIATED AMPHIPHILIC REPRESSION (EAR) motif and can directly interact with TPL, the remaining JAZ proteins lack an EAR domain and interact via their ZIM domain with NOVEL INTERACTOR OF JAZ (NINJA), an EAR motif-containing adaptor protein

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“…GsCID1 is responsible for 100-seed weight in wild soybean . Recently, GmKIX8-1 was demonstrated to be associated with soybean seed weight (Nguyen et al 2021). However, the molecular mechanism controlling yield in soybean is largely unknown.…”

Section: Introductionmentioning

confidence: 99%

GmFULa Improves Soybean Yield by Enhancing Carbon Assimilation without Altering Flowering Time or Maturity

Yue

Sun

et al. 2021

Preprint

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Soybean is highly demanded by a continuously growing human population. However, increasing soybean yield is a major challenge. FRUITFULL ( FUL ), a MADS-box transcription factor, plays important roles in multiple developmental processes, especially fruit and pod development, which are crucial for soybean yield formation. However, the functions of its homologs in soybean are not clear. Here, through haplotypes analysis, we found that haplotypes H02 of the soybean homolog GmFULa ( GmFULa-H02 ) is dominant in cultivated soybeans, suggesting that GmFULa-H02 was highly selected during domestication and varietal improvement of soybean. Interestingly, transgenic overexpression of GmFULa enhanced vegetative growth with more biomass accumulated and ultimately increased the yield but without affecting the plant height or changing the flowering time and maturity, indicating that it enhances the efficiency of dry matter accumulation. It also promoted the yield factors like branch number, pod number and 100-seed weight, which ultimately increased the yield. It increased the palisade tissue cell number and the chlorophyll content to promote photosynthesis and increase the soluble sugar content in leaves and fresh seeds. Furthermore, GmFULa were found to be sublocalized in the nucleus and positively regulate sucrose synthases ( SUSs ) and sucrose transporters ( SUTs ) by binding with the conserved CArG boxes in their promoters. Overall, these results showed GmFULa promotes the capacity of assimilation and the transport of the resultant assimilates to increase yield, and provided insights into the link between GmFULa and sucrose synthesis with transport related molecular pathways that control seed yield.

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GmKIX8‐1 regulates organ size in soybean and is the causative gene for the major seed weight QTL qSw17‐1

Cited by 67 publications

References 84 publications

A Major and Stable Quantitative Trait Locus qSS2 for Seed Size and Shape Traits in a Soybean RIL Population

A Major and Stable Quantitative Trait Locus qSS2 for Seed Size and Shape Traits in a Soybean RIL Population

The PEAPOD Pathway and Its Potential To Improve Crop Yield

GmFULa Improves Soybean Yield by Enhancing Carbon Assimilation without Altering Flowering Time or Maturity

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