GmDREB2, a soybean DRE-binding transcription factor, conferred drought and high-salt tolerance in transgenic plants

Chen, Ming; Wang, Qiaoyan; Cheng, Xuesen; Xu, Zhao-Shi; Li, Lian-Cheng; Ye, Xingguo; Xia, Lanqin; Ma, Yupo

doi:10.1016/j.bbrc.2006.12.027

Cited by 353 publications

(221 citation statements)

References 14 publications

Supporting

Mentioning

201

Contrasting

Unclassified

Order By: Relevance

“…A similar phenotype was observed when AtDREB1A was overexpressed in tobacco (Kasuga et al, 2004) and chrysanthemum (Hong et al, 2006). Heterologous expression of GmDREB2 resulted in improved tolerance to drought and high-salinity stresses in transgenic Arabidopsis (Chen et al, 2007). Similarly, GhDREB from cotton expressed in wheat conferred enhanced tolerance to drought, high salt, and freezing stresses in transgenic wheat (Gao et al, 2009).…”

Section: Ap2/erf Tf Familysupporting

confidence: 56%

The Potential of Transcription Factor-Based Genetic Engineering in Improving Crop Tolerance to Drought

Rabara¹,

Tripathi

Rushton³

2014

OMICS: A Journal of Integrative Biology

View full text Add to dashboard Cite

Drought is one of the major constraints in crop production and has an effect on a global scale. In order to improve crop production, it is necessary to understand how plants respond to stress. A good understanding of regulatory mechanisms involved in plant responses during drought will enable researchers to explore and manipulate key regulatory points in order to enhance stress tolerance in crops. Transcription factors (TFs) have played an important role in crop improvement from the dawn of agriculture. TFs are therefore good candidates for genetic engineering to improve crop tolerance to drought because of their role as master regulators of clusters of genes. Many families of TFs, such as CCAAT, homeodomain, bHLH, NAC, AP2/ERF, bZIP, and WRKY have members that may have the potential to be tools for improving crop tolerance to drought. In this review, the roles of TFs as tools to improve drought tolerance in crops are discussed. The review also focuses on current strategies in the use of TFs, with emphasis on several major TF families in improving drought tolerance of major crops. Finally, many promising transgenic lines that may have improved drought responses have been poorly characterized and consequently their usefulness in the field is uncertain. New advances in high-throughput phenotyping, both greenhouse and field based, should facilitate improved phenomics of transgenic lines. Systems biology approaches should then define the underlying changes that result in higher yields under water stress conditions. These new technologies should help show whether manipulating TFs can have effects on yield under field conditions.

show abstract

Section: Ap2/erf Tf Familysupporting

confidence: 56%

The Potential of Transcription Factor-Based Genetic Engineering in Improving Crop Tolerance to Drought

Rabara¹,

Tripathi

Rushton³

2014

OMICS: A Journal of Integrative Biology

View full text Add to dashboard Cite

show abstract

“…According to Sakuma et al (2002), the DREB subfamily of the AP2/ERF-type proteins is further classified into A-1 to A-6 subgroups, and DREB2A is a member of the A-2 subgroup. To date, several DREB subfamily transcription factors have been isolated from soybean and have been classified into subgroups A-1 (Glycine DREB1; Chen et al, 2006), A-2 (GmDREBa and GmDREBc; Li et al, 2005), and A-5 (GmDREB1-GmDREB3 and GmDREBb; Li et al, 2005;Chen et al, 2007Chen et al, , 2009b. We found that the A-2 subgroup, as well as most of the other subgroups in the DREB and ERF subfamilies, had been already established in an early stage of land plant evolution, suggesting functional differentiation between these subgroups .…”

mentioning

confidence: 99%

GmDREB2A;2, a Canonical DEHYDRATION-RESPONSIVE ELEMENT-BINDING PROTEIN2-Type Transcription Factor in Soybean, Is Posttranslationally Regulated and Mediates Dehydration-Responsive Element-Dependent Gene Expression

et al. 2012

View full text Add to dashboard Cite

Soybean (Glycine max) is an important crop around the world. Abiotic stress conditions, such as drought and heat, adversely affect its survival, growth, and production. The DEHYDRATION-RESPONSIVE ELEMENT-BINDING PROTEIN2 (DREB2) group includes transcription factors that contribute to drought and heat stress tolerance by activating transcription through the cis-element dehydration-responsive element (DRE) in response to these stress stimuli. Two modes of regulation, transcriptional and posttranslational, are important for the activation of gene expression by DREB2A in Arabidopsis (Arabidopsis thaliana). However, the regulatory system of DREB2 in soybean is not clear. We identified a new soybean DREB2 gene, GmDREB2A;2, that was highly induced not only by dehydration and heat but also by low temperature. GmDREB2A;2 exhibited a high transactivation activity via DRE and has a serine/threonine-rich region, which corresponds to a negative regulatory domain of DREB2A that is involved in its posttranslational regulation, including destabilization. Despite the partial similarity between these sequences, the activity and stability of the GmDREB2A;2 protein were enhanced by removal of the serine/threonine-rich region in both Arabidopsis and soybean protoplasts, suggestive of a conserved regulatory mechanism that involves the recognition of serine/ threonine-rich sequences with a specific pattern. The heterologous expression of GmDREB2A;2 in Arabidopsis induced DREregulated stress-inducible genes and improved stress tolerance. However, there were variations in the growth phenotypes of the transgenic Arabidopsis, the induced genes, and their induction ratios between GmDREB2A;2 and DREB2A. Therefore, the basic function and regulatory machinery of DREB2 have been maintained between Arabidopsis and soybean, although differentiation has also occurred.

show abstract

“…Nakashima et al, (2014), demostraron que la sobre-expresión de DREB1A mejora tolerancia a frío y deshidratación, mientras que la sobreexpresión de DREB2ACA mejora la tolerancia a deshidratación, pero levemente al frío en plantas transgénicas de Arabidopsis. En contraste, la expresión ectópica constitutiva o inducible por estrés del ZmDREB2A de maíz (Qin et al, 2007) y de GmDREB2 de soya (Gycine max) (Chen et al, 2007) en Arabidopsis resulta en mejora en la tolerancia a sequía, calor y estrés salino de plantas transgénicas sin defectos en el crecimiento, se sugiere que ninguna requiere de modificaciones post-traduccionales . Morran et al, (2011), transformaron cebada (Hordeum vulgare) y trigo (Triticum aestivum) con TaDREB3 y TaDREB2 y evaluaron tolerancia a sequía y heladas con un promotor constitutivo (2X 35S) en cebada e inducible (pRab17) en trigo.…”

Section: Discusión Genes De Tolerancia a Sequía Y Glufosinato De Amonunclassified

Diseño de casetes de expresión que confieran tolerancia a sequía y a glufosinato en maíz (Zea mays)

Carreño-Venegas

Chaparro-Giraldo

2016

Acta biol. Colomb.

View full text Add to dashboard Cite

RESUMENComo primera aproximación en la obtención de una línea transgénica de maíz tolerante a sequía y al herbicida glufosinato de amonio, se seleccionaron genes y elementos reguladores para el diseño in silico de casetes de expresión, a través del análisis de literatura científica y bases de datos de genes y patentes. Las secuencias génicas fueron modificadas con base en el criterio de uso codónico del maíz para optimizar su expresión. Los casetes de expresión diseñados con el software DNA 2.0., fueron sintetizados por una empresa especializada. La presencia del transgen y la expresión a nivel de mARN fue demostrada mediante PCR y RT-PCR en la planta modelo Nicotiana benthamiana transformada vía Agrobacterium tumefaciens. Un ensayo preliminar in vitro en condiciones simuladas de sequía en medio MS con PEG (PM 6000)10 % no demostró incremento notorio en la tolerancia de las plántulas transformantes, posiblemente debido a que el uso codónico del diseño no favorece la expresión génica en la planta modelo.Palabras clave: estrés abiótico, tolerancia a herbicida, transgénesis, uso codónico. ABSTRACTAs a first approach in obtaining a transgenic drought and glufosinate ammonium tolerant maize line, genes and regulatory elements for the in silico design of the expression cassettes were selected through analysis of scientific literature and databases of genes and patents. Gene sequences were modified based on the criterion of maize codon usage to optimize their expression. The constructs designed with DNA 2.0 Software, were synthesized by a specialized company. The presence of the transgene and the expression of mRNA was demonstrated by PCR and RT-PCR in the model plant Nicotiana benthamiana transformed via Agrobacterium tumefaciens. A preliminary experiment in vitro under simulated drought conditions in MS medium with 10 % PEG (PM 6000) showed no noticeable increase in drought tolerance of the transformants, possibly because the codon usage of the design does not promote gene expression in the model plant.

show abstract

GmDREB2, a soybean DRE-binding transcription factor, conferred drought and high-salt tolerance in transgenic plants

Cited by 353 publications

References 14 publications

The Potential of Transcription Factor-Based Genetic Engineering in Improving Crop Tolerance to Drought

The Potential of Transcription Factor-Based Genetic Engineering in Improving Crop Tolerance to Drought

GmDREB2A;2, a Canonical DEHYDRATION-RESPONSIVE ELEMENT-BINDING PROTEIN2-Type Transcription Factor in Soybean, Is Posttranslationally Regulated and Mediates Dehydration-Responsive Element-Dependent Gene Expression

Diseño de casetes de expresión que confieran tolerancia a sequía y a glufosinato en maíz (Zea mays)

Contact Info

Product

Resources

About