GmCLC1 Confers Enhanced Salt Tolerance through Regulating Chloride Accumulation in Soybean

Abstract

The family of chloride channel proteins that mediate Cl- transportation play vital roles in plant nutrient supply, cellular action potential and turgor pressure adjustment, stomatal movement, hormone signal recognition and transduction, Cl- homeostasis, and abiotic and biotic stress tolerance. The anionic toxicity, mainly caused by chloride ions (Cl-), on plants under salt stress remains poorly understood. In this work, we investigated the function of soybean Cl-/H+ antiporter GmCLC1 under salt stress in transgenic Arabidopsis thaliana, soybean, and yeast. We found that GmCLC1 enhanced salt tolerance in transgenic A. thaliana by reducing the Cl- accumulation in shoots and hence released the negative impact of salt stress on plant growth. Overexpression of GmCLC1 in the hairy roots of soybean sequestered more Cl- in their roots and transferred less Cl- to their shoots, leading to lower relative electrolyte leakage values in the roots and leaves. When either the soybean GmCLC1 or the yeast chloride transporter gene, GEF1, was transformed into the yeast gef1 mutant, and then treated with different chloride salts (MnCl2, KCl, NaCl), enhanced survival rate was observed. The result indicates that GmCLC1 and GEF1 exerted similar effects on alleviating the stress of diverse chloride salts on the yeast gef1 mutant. Together, this work suggests a protective function of GmCLC1 under Cl- stress.

Systematic hormone-metabolite network provides insights of high salinity tolerance inPongamia pinnata(L.) pierre

“…However, with increasing salt stress treatment time, the marginal expression of HKT1:1genemay regulate the retrevial of Na + from the xylem (Davenport et al, 2007; Ali et al, 2016). The expression levels of CLC1 indicates that CLC1 may not be involved in the Cl - vacuolar sequestation (Wei et al, 2016).…”

Transcriptomic Profiling Identifies Candidate Genes Involved in the Salt Tolerance of the Xerophyte Pugionium cornutum

“…AtCLCg is a unique protein that mediates Cl − compartmentalization into vacuoles of mesophyll cells in Arabidopsis [33]. In the typical Cl − -sensitive plant Glycine max, GmCLC1, a homolog of AtCLCg is preferentially expressed at the tonoplast of root cells, and thus the main function of GmCLC1 is to withhold Cl − in roots to decrease Cl − accumulation in shoots [34]. In the present study, the transcript abundance of P. cornutum CLCg in shoots was enhanced under salt treatment at both 6 and 24 h, as seen in Table 3.…”

Foliar 2,3-dihydroporphyrin iron (III) spray confers ameliorative antioxidation, ion redistribution and seed traits of salt-stressed soybean plants

“…Numerous transgenic studies have suggested that SOS1 as a Na + efflux transporter may be considered as the most important functional protein for the acquirement of a salt tolerance trait for different plant species (Nie et al, 2015). Cluptake, transport and compartmentation are mediated by chloride channels (CLCs), which are mostly distributed in the tonoplast of cell vacuoles, for soybean as an example, Gm-CLC1 has been shown to locate at the tonoplast and play a crucial role for Cluptake and compartmentation in the vesicles and for Cl -/salt tolerance under saline conditions (Wong et al, 2013;Wei et al, 2016). A widely studied chlorophyll derivative, chlorophyllin (CHL), the sodium-copper salt and water-soluble analogue of the ubiquitous green pigment chlorophyll, has been shown to possesses higher antioxidant ability than AsA, GSH, mannitol, and tert-butanol at equimolar concentrations for protecting mitochondria, inhibiting the mutagenicity of various chemicals in bacteria or mice (Kamat et al, 2000).…”