Glyphosate biodegradation and potential soil bioremediation by Bacillus subtilis strain Bs-15

Yu, Xingjun; Yu, Tao; Yin, Guangbo; Dong, Qingling; An, Mengjie; Wang, H R; Ai, Chongyang

doi:10.4238/2015.november.18.37

Cited by 52 publications

(33 citation statements)

References 36 publications

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“…The expression of an evolved glycine oxidase variant from B. subtilis indeed enhances glyphosate resistance in alfalfa (Nicolia et al, 2014). Finally, the B. subtilis strain Bs-15, which was isolated from a pepper plant seem to be capable of degrading glyphosate (Yu et al, 2015). However, the glyphosate degradation pathway remains to be identified in this isolate.…”

Section: Introductionmentioning

confidence: 95%

Identification of the first glyphosate transporter by genomic adaptation

Wicke

Schulz

Lentes

et al. 2019

Environmental Microbiology

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Summary The soil bacterium Bacillus subtilis can get into contact with growth‐inhibiting substances, which may be of anthropogenic origin. Glyphosate is such a substance serving as a nonselective herbicide. Glyphosate specifically inhibits the 5‐enolpyruvyl‐shikimate‐3‐phosphate (EPSP) synthase, which generates an essential precursor for de novo synthesis of aromatic amino acids in plants, fungi, bacteria and archaea. Inhibition of the EPSP synthase by glyphosate results in depletion of the cellular levels of aromatic amino acids unless the environment provides them. Here, we have assessed the potential of B. subtilis to adapt to glyphosate at the genome level. In contrast to Escherichia coli, which evolves glyphosate resistance by elevating the production and decreasing the glyphosate sensitivity of the EPSP synthase, B. subtilis primarily inactivates the gltT gene encoding the high‐affinity glutamate/aspartate symporter GltT. Further adaptation of the gltT mutants to glyphosate led to the inactivation of the gltP gene encoding the glutamate transporter GltP. Metabolome analyses confirmed that GltT is the major entryway of glyphosate into B. subtilis. GltP, the GltT homologue of E. coli also transports glyphosate into B. subtilis. Finally, we found that GltT is involved in uptake of the herbicide glufosinate, which inhibits the glutamine synthetase.

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Section: Introductionmentioning

confidence: 95%

Identification of the first glyphosate transporter by genomic adaptation

Wicke

Schulz

Lentes

et al. 2019

Environmental Microbiology

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“…Percent degradation was calculated by the formula of Sawhney et al (2015) and Yu et al (2015) as follows: Degradation rate (%) = (a-b)/ a x 100, where a is the quantity of glyphosate in the treated sample (glyphosate + bacteria), and b is the quantity of glyphosate in control (only glyphosate)…”

Section: Assesment Of Degradationmentioning

confidence: 99%

“…Because of the impact of glyphosate, it is important to identify methods for enhancing glyphosate degradation. Many studies have shown that glyphosate can be degraded by microorganisms (rhizospheric and endophytic bacteria); some of them are member of plant growth promoting bacteria (Grosbard and Atkinson, 1985;Yu et al, 2015). Glyphosate biodegradation in soil by Pseudomonas sp and E. Coli have shown high efficiency compare with it naturally biodegradation (Permanasari et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

“…Glyphosate biodegradation in soil by Pseudomonas sp and E. Coli have shown high efficiency compare with it naturally biodegradation (Permanasari et al, 2005). Bacillus subtilis strain Bs-15 can significantly promote glyphosate degradation in soil and play an important role in the bioremediation of glyphosate-contaminated soils (Yu et al, 2015). Azospirillum sp.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Glyphosate biodegradation by plant growth promoting bacteria and their effect to paddy germination in glyphosate contaminated soil

Andriani

Aini

Hadiastono

2017

J.Degrade.Min.Land Manage.

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Abstract:Glyphosate is the most widely used herbicide in Indonesia. Glyphosate persistence between 55 days to 3 years. Widespread and uncontrolled use can cause weeds to become resistant and residue contaminates the soil and water environment. Due to the residual impact of glyphosate, it is necessary to identify a method that can increase the degradation of glyphosate. Several studies have shown that glyphosate can be degraded by microorganisms (fungi, rhizosphere and endophytic bacteria), some of which are members of plant growth-promoting bacteria. This study used the bacteria Enterobacter cloacae, Enterobacter sp and Pseudomonas fluorescens. These three types of bacteria have growthpromoting properties and potentially increase glyphosate degradation. Results of chromatogram on the residual test of glyphosate in liquid medium and soil containing glyphosate showed that glyphosate residue decreased with the addition of bacterial treatment when compared to control. The percentage of degradation in liquid medium are 96.06% by Enterobacter cloacae, 57% by Enterobacter spand 93.45% by Pseudomonas fluorescens.The percentage of degradation in soil medium are 4.32% by Enterobacter cloacae, 23.49% by Enterobacter spand 12.19% by Pseudomonas fluorescens.A positive result indicates that bacterial growth boosters from the plant (endophyte) as well as the area of rooting (rhizosphere) have additional potential as biofertilizer, bio stimulant, bio protectant but also as bio degradator pollutants such as the herbicide glyphosate.

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“…Glyphosate is the chemical substance of What may affect the degradation rate of the herbicide in the earth is the presence of inorganic phosphates, which inhibit the action of some strains of bacteria from entering into competition with the molecule [17,18]. In fact, glyphosate is absorbed from the ground as well as through metal complexes and through the bonds created by the phosphorus atom.…”

Section: Introductionmentioning

confidence: 99%

Critical Review of the Effects of Glyphosate Exposure to the Environment and Humans through the Food Supply Chain

et al. 2018

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Abstract:Glyphosate is a synthesis product and chemical substance that entered in the global market during the 70s. In the beginning, the molecule was used as an active principle in a wide range of herbicides, with great success. This was mainly due to its systemic and non-selective action against vegetable organisms and also to the spread of Genetically Modified Organism (GMO) crops, which over the years were specifically created with a resistance to glyphosate. To date, the product is, for these reasons, the most sprayed and most used herbicide in the world. Because of its widespread diffusion into the environment, it was not long before glyphosate found itself at the center of an important scientific debate about its adverse effects on health and environment. In fact, in 2015 the IARC (International Agency for Research on Cancer, Lyon, France), an organization referred to as the specialized cancer agency of the World Health Organization (WHO, Geneva, Switzerland), classified the substance as "likely carcinogenic" to humans. This triggered an immediate and negative reaction from the producer, who accused the Agency and claimed that they had failed to carry out their studies properly and that these conclusions were largely contradictory to published research. Additionally, in 2015, just a few months after the IARC monography published on glyphosate, the EFSA (European Food Safety Authority, Parma, Italy), another WHO related organization, declared that it was "unlikely" that the molecule could be carcinogenic to humans or that it could cause any type of risk to human health. The conflict between the two organizations of the World Health Organization triggered many doubts, and for this reason, a series of independent studies were launched to better understand what glyphosate's danger to humans and the environment really was. The results have brought to light how massive use of the herbicide has created over time a real global contamination that has not only affected the soil, surface and groundwater as well as the atmosphere, but even food and commonly used objects, such as diapers, medical gauze, and absorbent for female intimate hygiene. How human health is compromised as a result of glyphosate exposure is a topic that is still very debatable and still unclear and unambiguous. This paper is a review of the results of the main independent recent scientific studies.

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Glyphosate biodegradation and potential soil bioremediation by Bacillus subtilis strain Bs-15

Cited by 52 publications

References 36 publications

Identification of the first glyphosate transporter by genomic adaptation

Identification of the first glyphosate transporter by genomic adaptation

Glyphosate biodegradation by plant growth promoting bacteria and their effect to paddy germination in glyphosate contaminated soil

Critical Review of the Effects of Glyphosate Exposure to the Environment and Humans through the Food Supply Chain

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