Glycyrrhizin Inhibits PEDV Infection and Proinflammatory Cytokine Secretion via the HMGB1/TLR4-MAPK p38 Pathway

Gao, Ruyi; Zhang, Yongshuai; Kang, Yuhui; Xu, Weiyin; Jiang, Lianhe; Guo, Tingting; Huan, Changchao

doi:10.3390/ijms21082961

Cited by 41 publications

(32 citation statements)

References 54 publications

(80 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The PEDV N antibody used herein was generated in our laboratory. The anti-PEDV-N polyclonal antibody was prepared in rabbits using PEDV-N protein as antigen and western blot and IFA were diluted 1:5,000 and 1:500, respectively ( Gao, et al, 2020 ). The β-actin antibody was purchased from TransGenBiotech (China).…”

Section: Methodsmentioning

confidence: 99%

“…The western blotting procedure followed previous descriptions ( Huan, et al, 2017 ; Gao, et al, 2020 ). PEDV N and actin were detected using the above-mentioned primary antibody and a secondary antibody.…”

Section: Methodsmentioning

confidence: 99%

“…IFAs were performed as per a previous description ( Gao, et al, 2020 ). Briefly, Vero cells were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 3% bovine serum albumin, and incubated with the anti-PEDV N antibody and an Alexa Fluor 5558-conjugated goat anti-rabbit secondary antibody (Invitrogen, United States).…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Epigallocatechin-3-Gallate, the Main Polyphenol in Green Tea, Inhibits Porcine Epidemic Diarrhea Virus In Vitro

Huan

et al. 2021

Front. Pharmacol.

Self Cite

View full text Add to dashboard Cite

There are currently no licensed drugs against porcine epidemic diarrhea virus (PEDV), but vaccines are available. We identified a natural molecule, epigallocatechin-3-gallate (EGCG), the main polyphenol in green tea, which is effective against infection with PEDV. We used a variety of methods to test its effects on PEDV in Vero cells. Our experiments show that EGCG can effectively inhibit PEDV infections (with HLJBY and CV777 strains) at different time points in the infection using western blot analysis. We found that EGCG inhibited PEDV infection in a dose-dependent manner 24 h after the infection commenced using western blotting, plaque formation assays, immunofluorescence assays (IFAs), and quantitative reverse-transcriptase PCR (qRT-PCR). We discovered that EGCG treatment of Vero cells decreased PEDV attachment and entry into them by the same method analysis. Western blotting also showed that PEDV replication was inhibited by EGCG treatment. Whereas EGCG treatment was found to inhibit PEDV assembly, it had no effect on PEDV release. In summary, EGCG acts against PEDV infection by inhibiting PEDV attachment, entry, replication, and assembly.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Epigallocatechin-3-Gallate, the Main Polyphenol in Green Tea, Inhibits Porcine Epidemic Diarrhea Virus In Vitro

Huan

et al. 2021

Front. Pharmacol.

Self Cite

View full text Add to dashboard Cite

show abstract

“…Gycyrrhizin. Unknown Pompei et al, 1979 Pompei et al, 1980 Sato et al, 1996 Cinatl J et al, 2003 Hoever et al, 2005 Wolkerstorfer et al, 2009 Michaelis et al, 2011 Dao et al, 2010 Ashfaq et al, 2011 Hardy et al, 2012 Matsumoto et al, 2013 Yu et al, 2014 Laconi et al, 2014 Duan et al, 2015 Baltina et al, 2015 Chen Q et al, 2017 Si et al, 2018 Liang et al, 2019 Baltina et al, 2019 Gao et al, 2020 Tong et al, 2020 Chen and Du, 2020 …”

Section: Antiviral Effect Of Glycyrrhizin and Licorice Extractmentioning

confidence: 99%

The potential of glycyrrhizin and licorice extract in combating COVID-19 and associated conditions

Gomaa

Abdel-Wadood

2021

Phytomedicine Plus

View full text Add to dashboard Cite

Background Several recent studies have stated that glycyrrhizin and licorice extract are present in most traditional Chinese medicine formulas used against SARS-CoV-2 in China. Significant data are showing that glycyrrhizin and licorice extract have multiple beneficial activities in combating most features of SARS-CoV-2 Purpose The aim of current review was to highlight recent progresses in research that showed the evidence of the potential use of glycyrrhizin and licorice extract against COVID-19. Methodology We have reviewed the information published from 1979 to October 2020. These studies demonstrated the effects , use and safety of glycyrrhizin and icorice extract against viral infections,bacterial infections, inflammatory disorders of lung ( in vitro and in vivo ). These studies were collated through online electronic databases research (Academic libraries as PubMed, Scopus, Web of Science and Egyptian Knowledge Bank). Results Pooled effect size of articles provides information about the rationale for using glycyrrhizin and licorice extract to treat COVID-19. Fifty studies demonstrate antiviral activity of glycyrrhizin and licorice extract. The most frequent mechanism of the antiviral activity is due to disrupting viral uptake into the host cells and disrupting the interaction between receptor- binding domain (RBD) of SARS-COV2 and ACE2 in recent articles. Fifty studies indicate that glycyrrhizin and licorice extract have significant antioxidant, anti-inflammatory and immunomodulatory effects. Twenty five studies provide evidence for the protective effect of glycyrrhizin and licorice extract against inflammation-induced acute lung injury and cardiovascular disorders. Conclusion The current study showed several evidence regarding the beneficial effects of glycyrrhizin and licorice extract in combating COVID-19. More randomized clinical trials are needed to obtain a precise conclusion.

show abstract

“…Studies further revealed that the inactivation of NF- κ B led to a reduced expression of different proinflammatory cytokines like IL-1 β , IL-6, TNF- α , and HMGB1 ( Figure 2 ) [ 129 , 139 , 140 ]. GL was capable of blocking porcine epidemic diarrhea virus infection, as well as reduced proinflammatory cytokine expression via the HMGB1/TLR4-p38MAPK pathway ( Figure 2 and Table 1 ) [ 141 ].…”

Section: Toll-like Receptorsmentioning

confidence: 99%

Exploring the Pivotal Immunomodulatory and Anti-Inflammatory Potentials of Glycyrrhizic and Glycyrrhetinic Acids

Richard¹

2021

Mediators of Inflammation

View full text Add to dashboard Cite

Licorice extract is a Chinese herbal medication most often used as a demulcent or elixir. The extract usually consists of many components but the key ingredients are glycyrrhizic (GL) and glycyrrhetinic acid (GA). GL and GA function as potent antioxidants, anti-inflammatory, antiviral, antitumor agents, and immuneregulators. GL and GA have potent activities against hepatitis A, B, and C viruses, human immunodeficiency virus type 1, vesicular stomatitis virus, herpes simplex virus, influenza A, severe acute respiratory syndrome-related coronavirus, respiratory syncytial virus, vaccinia virus, and arboviruses. Also, GA was observed to be of therapeutic valve in human enterovirus 71, which was recognized as the utmost regular virus responsible for hand, foot, and mouth disease. The anti-inflammatory mechanism of GL and GA is realized via cytokines like interferon-γ, tumor necrotizing factor-α, interleukin- (IL-) 1β, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12, and IL-17. They also modulate anti-inflammatory mechanisms like intercellular cell adhesion molecule 1 and P-selectin, enzymes like inducible nitric oxide synthase (iNOS), and transcription factors such as nuclear factor-kappa B, signal transducer and activator of transcription- (STAT-) 3, and STAT-6. Furthermore, DCs treated with GL were capable of influencing T-cell differentiation toward Th1 subset. Moreover, GA is capable of blocking prostaglandin-E2 synthesis via blockade of cyclooxygenase- (COX-) 2 resulting in concurrent augmentation nitric oxide production through the enhancement of iNOS2 mRNA secretion in Leishmania-infected macrophages. GA is capable of inhibiting toll-like receptors as well as high-mobility group box 1.

show abstract

Glycyrrhizin Inhibits PEDV Infection and Proinflammatory Cytokine Secretion via the HMGB1/TLR4-MAPK p38 Pathway

Cited by 41 publications

References 54 publications

Epigallocatechin-3-Gallate, the Main Polyphenol in Green Tea, Inhibits Porcine Epidemic Diarrhea Virus In Vitro

Epigallocatechin-3-Gallate, the Main Polyphenol in Green Tea, Inhibits Porcine Epidemic Diarrhea Virus In Vitro

The potential of glycyrrhizin and licorice extract in combating COVID-19 and associated conditions

Exploring the Pivotal Immunomodulatory and Anti-Inflammatory Potentials of Glycyrrhizic and Glycyrrhetinic Acids

Contact Info

Product

Resources

About