Epigallocatechin-3-Gallate, the Main Polyphenol in Green Tea, Inhibits Porcine Epidemic Diarrhea Virus In Vitro

Huan, Changchao; Xu, Weiyin; Ni, Bo; Guo, Tingting; Pan, Haochun; Jiang, Lianhe; Li, Lin; Yao, Jingting; Gao, Song

doi:10.3389/fphar.2021.628526

Cited by 17 publications

(17 citation statements)

References 63 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our previous studies have shown that the small molecular medicine EGCG can inhibit PRV and PEDV adsorption, entry, and replication [ 20 , 40 ]. However, most of the small molecule medicines can only act on a single target.…”

Section: Discussionmentioning

confidence: 99%

Antiviral Activity of Plantago asiatica Polysaccharide against Pseudorabies Virus In Vitro

Huan

Zhang

et al. 2022

Oxidative Medicine and Cellular Longevity

Self Cite

View full text Add to dashboard Cite

Pseudorabies (PR) is an acute infectious disease of various domestic animals and wild animals caused by pseudorabies virus (PRV). It is mainly characterized by fever, itching, encephalomyelitis, and respiratory and neurological disorders. Plantago asiatica polysaccharide (PLP), extracted from the whole plant of Plantago asiatica L., showed immunomodulatory and antioxidation effects, but the antiviral activity had not been reported. In this study, the inhibitory effect of PLP on PRV infection was studied. Our study first revealed that PLP could inhibit PRV infection in a dose-dependent manner. By adding PLP at different stages of the virus’s life cycle, we revealed that PLP could reduce the attachment and penetration of PRV into PK15 cells. The inhibition of PRV attachment was better than inhibition of PRV penetration. However, PLP did not affect PRV replication and inactivation. In addition, PLP decreased the intracellular ROS levels in infected cells significantly, and ROS scavenger NAC decreased PRV infection. Therefore, our study provided preliminary data of anti-PRV activity of PLP, which was established to be a novel anti-PRV infection agent.

show abstract

Section: Discussionmentioning

confidence: 99%

Antiviral Activity of Plantago asiatica Polysaccharide against Pseudorabies Virus In Vitro

Huan

Zhang

et al. 2022

Oxidative Medicine and Cellular Longevity

Self Cite

View full text Add to dashboard Cite

show abstract

“…Salinomycin [ 39 ] and remdesivir [ 40 ] have been reported as potential antiviral drugs against PEDV infection in vitro. Several natural products extracted from plants, such as glycyrrhizin [ 16 ], quercetin 7-rhamnoside [ 15 ], and epigallocatechin-3-gallate [ 41 ], have been demonstrated to reduce PEDV infection in Vero cells. To search for new anti-PEDV agents, Deejai et al screened the FDA-approved drugs that could associate with the PEDV N protein using virtual screening and found that trichlormethiazide, D-(+) biotin and acetazolamide could inhibit PEDV replication in vitro [ 42 ].…”

Section: Discussionmentioning

confidence: 99%

Construction of a Recombinant Porcine Epidemic Diarrhea Virus Encoding Nanoluciferase for High-Throughput Screening of Natural Antiviral Products

Wan

Zhang

Zheng

et al. 2021

Viruses

View full text Add to dashboard Cite

Porcine epidemic diarrhea virus (PEDV) is the predominant cause of an acute, highly contagious enteric disease in neonatal piglets. There are currently no approved drugs against PEDV infection. Here, we report the development of a nanoluciferase (NLuc)-based high-throughput screening (HTS) platform to identify novel anti-PEDV compounds. We constructed a full-length cDNA clone for a cell-adapted PEDV strain YN150. Using reverse genetics, we replaced the open reading frame 3 (ORF3) in the viral genome with an NLuc gene to engineer a recombinant PEDV expressing NLuc (rPEDV-NLuc). rPEDV-NLuc produced similar plaque morphology and showed similar growth kinetics compared with the wild-type PEDV in vitro. Remarkably, the level of luciferase activity could be stably detected in rPEDV-NLuc-infected cells and exhibited a strong positive correlation with the viral titers. Given that NLuc expression represents a direct readout of PEDV replication, anti-PEDV compounds could be easily identified by quantifying the NLuc activity. Using this platform, we screened for the anti-PEDV compounds from a library of 803 natural products and identified 25 compounds that could significantly inhibit PEDV replication. Interestingly, 7 of the 25 identified compounds were natural antioxidants, including Betulonic acid, Ursonic acid, esculetin, lithocholic acid, nordihydroguaiaretic acid, caffeic acid phenethyl ester, and grape seed extract. As expected, all of the antioxidants could potently reduce PEDV-induced oxygen species production, which, in turn, inhibit PEDV replication in a dose-dependent manner. Collectively, our findings provide a powerful platform for the rapid screening of promising therapeutic compounds against PEDV infection.

show abstract

“…The hemolytic activity and mammalian cell cytotoxicity of metformin in combination with minocycline was evaluated based on previously reported studies [ 46 , 52 ]. For hemolytic activity assay, 8% sheep blood cells (Yuanye, Shanghai, China) prepared from fresh sterile defibrinated sheep blood was treated with a combination of minocycline (16–128 µg/mL) and metformin (0–80 mg/mL), using 0.2% Triton X-100 as a positive control and PBS (0.01 mol/L, pH = 7.4) as a negative control at 37 °C for one hour followed by the measurement of the released hemoglobin absorption at 576 nm by Bio-Tek Synergy2 Multi-mode Microplate Reader.…”

Section: Methodsmentioning

confidence: 99%

Metformin reverse minocycline to inhibit minocycline-resistant Acinetobacter baumannii by destroy the outer membrane and enhance membrane potential in vitro

et al. 2022

Self Cite

View full text Add to dashboard Cite

Background Acinetobacter baumannii (A. baumannii) is an opportunistic pathogen and has emerged as one of the most troublesome pathogens. Drug resistance in A. baumannii has been reported on a global scale. Minocycline was found to be active against multi-drug resistant A. baumannii and was approved by the FDA for the infections caused by sensitive strains of A. baumannii. However, the emergence of minocycline resistance and its toxic effects still need to be addressed. Therefore, this study aimed to evaluate the synergistic effects of metformin combined with minocycline on minocycline-resistant A. baumannii. Results The effect of metformin on the antibacterial activity of minocycline was determined by checkerboard and time-killing assay. Further, it was observed by biofilm formation assay that metformin combination with minocycline can inhibit the formation of biofilm. Outer membrane integrity, membrane permeability, membrane potential and reactive oxygen species (ROS) were monitored to explore the underlying synergistic mechanisms of metformin on minocycline. And the results shown that metformin can destroy the outer membrane of A. baumannii, enhance its membrane potential, but does not affect the membrane permeability and ROS. Conclusion These findings suggested that the combination of metformin and minocycline has the potential for rejuvenating the activity of minocycline against minocycline-resistant A. baumannii.

show abstract

Epigallocatechin-3-Gallate, the Main Polyphenol in Green Tea, Inhibits Porcine Epidemic Diarrhea Virus In Vitro

Cited by 17 publications

References 63 publications

Antiviral Activity of Plantago asiatica Polysaccharide against Pseudorabies Virus In Vitro

Antiviral Activity of Plantago asiatica Polysaccharide against Pseudorabies Virus In Vitro

Construction of a Recombinant Porcine Epidemic Diarrhea Virus Encoding Nanoluciferase for High-Throughput Screening of Natural Antiviral Products

Metformin reverse minocycline to inhibit minocycline-resistant Acinetobacter baumannii by destroy the outer membrane and enhance membrane potential in vitro

Contact Info

Product

Resources

About