Glycyrrhizin Attenuates MPTP Neurotoxicity in Mouse and MPP<sup>+</sup>-Induced Cell Death in PC12 Cells

Kim, Yun Jeong; Lee, Chung Soo

doi:10.4196/kjpp.2008.12.2.65

Cited by 31 publications

(22 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Mice were killed 48 h after the last injection. For the stereotaxic injection group, mice were intraperitoneally injected with MPTP three days after virus delivery [ 26 ].…”

Section: Methodsmentioning

confidence: 99%

The C‐ETS2‐TFEB Axis Promotes Neuron Survival under Oxidative Stress by Regulating Lysosome Activity

Fang

Luo

et al. 2016

Oxidative Medicine and Cellular Longevity

View full text Add to dashboard Cite

Excessive reactive oxygen species/reactive nitrogen species (ROS/RNS) produced as a result of ageing causes damage to macromolecules and organelles or leads to interference of cell signalling pathways, which in turn results in oxidative stress. Oxidative stress occurs in many neurodegenerative diseases (e.g., Parkinson's disease) and contributes to progressive neuronal loss. In this study, we show that cell apoptosis is induced by oxidative stress and that lysosomes play an important role in cell survival under oxidative stress. As a compensatory response to this stress, lysosomal genes were upregulated via induction of transcription factor EB (TFEB). In addition, localization of TFEB to the nucleus was increased by oxidative stress. We also confirmed that TFEB protects cells from oxidative stress both in vitro and in vivo. Finally, we found that C-ETS2 senses oxidative stress, activates TFEB transcription, and mediates the upregulation of lysosomal genes. Our results demonstrate a mechanistic pathway for inducing lysosomal activity during ageing and neurodegeneration.

show abstract

“…Mice were killed 48 h after the last injection. For the stereotaxic injection group, mice were intraperitoneally injected with MPTP three days after virus delivery [ 26 ].…”

Section: Methodsmentioning

confidence: 99%

The C‐ETS2‐TFEB Axis Promotes Neuron Survival under Oxidative Stress by Regulating Lysosome Activity

Fang

Luo

et al. 2016

Oxidative Medicine and Cellular Longevity

View full text Add to dashboard Cite

show abstract

“…Subsequently, as a feed-back regulation mechanism, triterpenoids are produced as an antioxidant to scavenge ROS (Okubo and Yoshiki, 2000). There are many pieces of evidence indicating that glycyrrhizin and its hydrolysed metabolite 18b-glycyrrhetinic acid exert antioxidant effects (Kim and Lee, 2008). In Bupleurum spp., an incremental effect of drought stress on the levels of triterpenoids was also reported (Zhu et al, 2009).…”

Section: Glycyrrhizin Content Mg/g Air-dried Weightmentioning

confidence: 99%

“…Under drought stress, the concentration of enzymatic and nonenzymatic antioxidants tended to increase (Pan et al, 2006). Increasing evidence indicates that glycyrrhizin, a triterpenoid saponin found in G. glabra, exerts an anti-oxidant effect, reducing oxidative damage (Kim and Lee, 2008).…”

Section: Glycyrrhizin Content Mg/g Air-dried Weightmentioning

confidence: 99%

See 1 more Smart Citation

The effect of drought stress on the expression of key genes involved in the biosynthesis of triterpenoid saponins in liquorice ( Glycyrrhiza glabra )

et al. 2014

View full text Add to dashboard Cite

Glycyrrhiza glabra is an important medicinal plant throughout the world. Glycyrrhizin is a triterpenoid that is among the most important secondary metabolites produced by liquorice. Drought stress is proposed to enhance the levels of secondary metabolites. In this study, the effect of drought stress on the expression of important genes involved in the glycyrrhizin biosynthetic pathway was examined. Drought stress at the seedling stage was applied to 8-day-old plants using polyethylene glycol. Subsequently, the samples were collected 0, 4, 8 or 24 h post-treatment. At the adult plant stage, 10-month-old plants were subjected to drought stress by discontinuing irrigation. Subsequently, samples were collected at 2, 16 and 28 days after drought imposition (S(2d), S(16d) and S(28d), respectively). We performed semi-quantitative RT-PCR assays to evaluate the gene expression levels of sequalene synthase (SQS), β-amyrin synthase (bAS), lupeol synthase (LUS) and cycloartenol synthase (CAS) during stress. Finally, the glycyrrhizin content of stolons was determined via HPLC. The results revealed that due to osmotic stress, the gene expression levels of SQS and bAS were increased, whereas those of CAS were relatively unchanged at the seedling stage. At the adult plant stage, the expression levels of SQS and bAS were increased under drought stress conditions, whereas the gene expression level of CAS remained relatively constant. The glycyrrhizin content in stolons was increased only under severe drought stress conditions (S(28d)). Our results indicate that application of controlled drought stress up-regulates the expression of key genes involved in the biosynthesis of triterpenoid saponins and directly enhances the production of secondary metabolites, including glycyrrhizin, in liquorice plants.

show abstract

“…Triterpenoids are associated with antioxidant activities (Okubo and Yoshiki 2000). Glycyrrhizin (triterpenoid) acting as a ROS depreciator, plays a crucial role in contesting the oxidative stress thus minimizes the negative effect of drought stress (Kim and Lee 2008). Isopentenyl pyrophosphate, a precursor of terpene biosynthesis, was enhanced under drought stress (Turtola et al 2003).…”

Section: Discussionmentioning

confidence: 99%

Elicitation Enhanced the Yield of Glycyrrhizin and Antioxidant Activities in Hairy Root Cultures of Glycyrrhiza glabra L.

Srivastava

Singh

Sharma

et al. 2018

J Plant Growth Regul

View full text Add to dashboard Cite

Glycyrrhiza glabra L. has become an endangered medicinal plant due to the unabated extraction of glycyrrhizin. Glycyrrhizin is a triterpenoid saponin that is a root centric secondary metabolite having numerous pharmacological properties, such as anti-inflammatory, immunomodulatory, antiallergic, antiulcer, and is found to be effective even against HIV. Harvesting of the roots for high value glycyrrhizin destroys the whole plant causing existential threat to the plant itself and consequent damage to biodiversity. The present study establishes that hairy root cultures of G. glabra, using an optimized elicitor, can dramatically enhance focused production of glycyrrhizin at a much faster pace year-round without causing destruction of the plant. Hairy root cultures of G. glabra were developed using the Agrobacterium rhizogenes A4 strain. The glycyrrhizin content was enhanced using different biotic and abiotic elicitors, for example, PEG (polyethylene glycol), CdCl 2 , cellulase, and mannan at different concentrations and durations. PEG at 1% concentration enhanced the yield of glycyrrhizin up to 5.4fold after 24 h of exposure, whereas 200 µg mL −1 cellulase enhanced glycyrrhizin yield to 8.6-fold after 7 days of treatment. Mannan at 10 mg L −1 concentration enhanced the production of glycyrrhizin up to 7.8-fold after 10 days of stress. Among different antioxidant enzymes, SOD activity was significantly enhanced under drought, cellulase and mannan stress. This identification of elicitors can result in abundant supply of valuable glycyrrhizin to meet broad spectrum demand through commercial production without endangering G. glabra L.

show abstract

Glycyrrhizin Attenuates MPTP Neurotoxicity in Mouse and MPP⁺-Induced Cell Death in PC12 Cells

Abstract: The present study examined the inhibitory effect of licorice compounds glycyrrhizin and a metabolite 18β -glycyrrhetinic acid on the neurotoxicity of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (

Cited by 31 publications

References 32 publications

The C‐ETS2‐TFEB Axis Promotes Neuron Survival under Oxidative Stress by Regulating Lysosome Activity

The C‐ETS2‐TFEB Axis Promotes Neuron Survival under Oxidative Stress by Regulating Lysosome Activity

The effect of drought stress on the expression of key genes involved in the biosynthesis of triterpenoid saponins in liquorice ( Glycyrrhiza glabra )

Elicitation Enhanced the Yield of Glycyrrhizin and Antioxidant Activities in Hairy Root Cultures of Glycyrrhiza glabra L.

Contact Info

Product

Resources

About

Glycyrrhizin Attenuates MPTP Neurotoxicity in Mouse and MPP+-Induced Cell Death in PC12 Cells

Abstract: The present study examined the inhibitory effect of licorice compounds glycyrrhizin and a metabolite 18β -glycyrrhetinic acid on the neurotoxicity of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (

Cited by 31 publications

References 32 publications

The C‐ETS2‐TFEB Axis Promotes Neuron Survival under Oxidative Stress by Regulating Lysosome Activity

The C‐ETS2‐TFEB Axis Promotes Neuron Survival under Oxidative Stress by Regulating Lysosome Activity

The effect of drought stress on the expression of key genes involved in the biosynthesis of triterpenoid saponins in liquorice ( Glycyrrhiza glabra )

Elicitation Enhanced the Yield of Glycyrrhizin and Antioxidant Activities in Hairy Root Cultures of Glycyrrhiza glabra L.

Contact Info

Product

Resources

About

Glycyrrhizin Attenuates MPTP Neurotoxicity in Mouse and MPP⁺-Induced Cell Death in PC12 Cells