1998
DOI: 10.1074/jbc.273.4.2355
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Glycosylphosphatidylinositol Anchors of Membrane Glycoproteins Are Binding Determinants for the Channel-forming Toxin Aerolysin

Abstract: Cells that are sensitive to the channel-forming toxin aerolysin contain surface glycoproteins that bind the toxin with high affinity. Here we show that a common feature of aerolysin receptors is the presence of a glycosylphosphatidylinositol anchor, and we present evidence that the anchor itself is an essential part of the toxin binding determinant. The glycosylphosphatidylinositol (GPI)-anchored T-lymphocyte protein Thy-1 is an example of a protein that acts as an aerolysin receptor. This protein retained its… Show more

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Cited by 179 publications
(185 citation statements)
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“…We have made similar observations with another cell line previously (Diep et al, 1998). Indicators of apoptosis, including DNA fragmentation (Fig.…”
Section: Low Concentrations Of Aerolysin Cause Apoptosissupporting
confidence: 86%
See 2 more Smart Citations
“…We have made similar observations with another cell line previously (Diep et al, 1998). Indicators of apoptosis, including DNA fragmentation (Fig.…”
Section: Low Concentrations Of Aerolysin Cause Apoptosissupporting
confidence: 86%
“…The toxin binds with high af®nity to several glycosylphosphatidylinositol (GPI)-anchored proteins on sensitive cells (Cowell et al, 1997;Nelson et al, 1997;Diep et al, 1998). On T lymphomas, these receptors include Thy-1 and at least one other GPI-anchored protein (Diep et al, 1998).…”
Section: Introductionmentioning
confidence: 99%
See 1 more Smart Citation
“…However, different receptors were found on different cells types and a given cell type was found to have more then one receptor. For example, aerolysin was shown to bind to Thy-1 as well as other GPI-anchored proteins on Tlymphocytes, to Thy-1 and contactin in mouse brain (11,13), to VSG from Trypanosomes (13), to an 47-kDa receptor on rat erythrocytes (12) and to mainly an 80-kDa receptor on baby hamster kidney cells (14). Binding was shown to be determined both by the protein moiety and the olisaccharides of the anchor (13).…”
mentioning
confidence: 99%
“…Proaerolysin reacts specifically with the GPI anchor component (43). If the PrP in AsPC-1 cell is GPI-anchored, it should react with proaerolysin.…”
Section: Prp From Aspc-1 But Not Bxpc-3 Is Sensitive To Pi-plc Treatmmentioning
confidence: 99%