Glycosylation of serum albumin: Elevated glycosyl‐albumin in diabetic patients

Dolhofer, Roswitha; Wieland, H.

doi:10.1016/0014-5793(79)81345-9

Cited by 168 publications

(48 citation statements)

References 15 publications

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“…Since the lifespan of erythrocytes is about 120 d, HbA1c reflects the glycemia for the recent few months [3] . Glycated albumin (GA) is another index of glycemic control which correlates with the plasma glucose levels during the past few weeks because the turnover of albumin is about 20 d [4,5] . Although the ratio of GA/HbA1c is usually close to 3, the value changes based on the patient's condition [6] .…”

Section: Introductionmentioning

confidence: 99%

Elevation of the glycated albumin to glycated hemoglobin ratio during the progression of hepatitis C virus related liver fibrosis

Aizawa¹,

Enomoto²,

Imanishi³

et al. 2012

WJH

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AIM: To analyze the relationship between the glycated albumin (GA) to glycated hemoglobin (HbA1c) ratio and the histological grading of liver fibrosis. METHODS: The study retrospectively included consecutive hepatitis C virus positive chronic liver disease patients (n = 142) who had undergone percutaneous liver biopsy between January 2008 and March 2010 at our institution. The ratios of GA/HbA1c were calculated in all patients to investigate the relationship with the degree of the liver fibrosis. The values of the aspartate aminotransferase-to-platelet ratio index (APRI), an excellent marker for the evaluation of liver fibrosis, were also calculated. In addition, we combined the ratio of GA/HbA1c and the APRI in order to improve our ability to detect the presence of significant liver fibrosis. RESULTS: Sixty-one (43%) patients had either no fibrosis or minimal fibrosis (METAVIR score: F0-F1), while 25 (17%) had intermediate fibrosis (F2). Fifty-six (39%) patients had severe fibrosis (F3-F4) and 27 of them had cirrhosis (F4). The mean values of the GA/HbA1c increased with the progression of the fibrosis (F0-1: 2.83 ± 0.24, F2: 2.85 ± 0.24, F3: 2.92 ± 0.35, F4: 3.14 ± 0.54). There was a significant difference between the F0-F1 vs F4, F2 vs F4, and F3 vs F4 groups (P < 0.01, P < 0.01, P < 0.01 and P < 0.05, respectively). The GA/HbA1c ratio was significantly higher in the patients with cirrhosis (F4) than in those without cirrhosis (F0-F3) (3.14 ± 0.54 vs 2.85 ± 0.28, P < 0.0001). The GA/HbA1c ratio was also significantly higher in the patients with severe fibrosis (F3-F4) than in those without severe liver fibrosis (F0-F2) (3.03 ± 0.41 vs 2.84 ± 0.24, P < 0.001). Furthermore, the GA/HbA1c ratio was also significantly higher in the patients with significant fibrosis (F2-F4) than in those without significant liver fibrosis (F0-F1) (2.98 ± 0.41 vs 2.83 ± 0.24, P < 0.001). The diagnostic performance of the increased GA/HbA1c ratio (> 3.0) was as follows: its sensitivity and specificity for the detection of liver cirrhosis (F4) were 59.3% and 70.4%, respectively and its sensitivity and specificity for the detection of severe liver fibrosis (F3-F4) were 50.0% and 74.4%, respectively. With regard to the detection of significant fibrosis (F2-F4), its sensitivity was 44.4% and its specificity was 77.0%. Although even the excellent marker APRI shows low sensitivity (25.9%) for distinguishing patients with or without significant fibrosis, the combination of the APRI and GA/HbA1c ratio increased the sensitivity up to 42.0%, with only a modest decrease in the specificity (from 90.2% to 83.6%). CONCLUSION: The GA/HbA1c ratio increased in line with the histological severity of liver fibrosis, thus suggesting that this ratio is useful as a supportive index of liver fibrosis

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Section: Introductionmentioning

confidence: 99%

Elevation of the glycated albumin to glycated hemoglobin ratio during the progression of hepatitis C virus related liver fibrosis

Aizawa¹,

Enomoto²,

Imanishi³

et al. 2012

WJH

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show abstract

“…A number of proteins like hemoglobin, serum albumin, globulins, collagen, lens crystallins, calmodulin, osteocaldn and lipoprotelns have been reported to be prone to glycation (1,2). The measurement of glycated hemoglobin has been suggested as an alternative to oral glucose tolerance test for diabetes screening (3,4).…”

Section: Introductionmentioning

confidence: 99%

Estimation of glycated hemoglobin by 2,6-dimethylphenol: Sulphuric acid conventional method

Mallya

Pattabiraman

2001

Indian J Clin Biochem

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Glycated hemoglobin levels in hemolysate of normal and diabetic patients were determined by the 2,6-dimnthylphenol:67.5% sulphudc acid conventional method and the values were 0.3930.025 and 0.5910.21 moles of hydroxymethylfurfural(HMF)/mole of globin, respectively. The mean increase in glyceted hemoglobin values in diabetics (1.8 fold) was highly significant (p<0.001). A good correlation (r--0.95) was found the glycated hemoglobin values obtained by this method and the phenol:sulphudc acid method. The values obtained by former method were about 1.2.1.4 times the values by the phenoi:sulphuric acid method. This study indicates that conventional 2,6-dimethylphenoh57.5% suiphuric acid method is more sensitive for the estimation of glycetsd hemoglobin than any other method based on the same principle, it is less time consuming, reliable and hence can be employed for the routine laboretoW estimation of glyceted hemoglobin for the assessment of glycemic control.

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“…Glycated proteins accumulate in patients with diabetes mellitus (DM) more than in normal subjects because plasma proteins of diabetic patients are exposed to high concentrations of glucose over a long period. 5,6) Glycated proteins provide information about the mean blood glucose level. [7][8][9] Glycated hemoglobin has been used widely as an index of diabetic control because it reflects blood glucose levels of the past 2-3 months; 7) fructosamine values, in contrast, reflect the status of diabetic control in the past 2-3 weeks.…”

mentioning

confidence: 99%

“…10,13,[16][17][18] Although the conventional method is based on non-specific reduction, the fructosamine value reflects mainly the glycation of albumin because albumin accounts for 55-80% of the glycated proteins in serum. 6,16) Therefore, it is assumed that a fructosamine assay using albumin (albumin fructosamine assay) can be useful. 10,13) The albumin fructosamine assay makes use of affi-gel blue 10) or polyethylene glycol 13) for the purification of albumin.…”

mentioning

confidence: 99%

Fructosamine Assay Using Albumin Extracted from Serum.

Ohkawara

Nohara

Kanno

et al. 2002

Biological & Pharmaceutical Bulletin

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Albumin extracted from serum by a simple technique using trichloroacetic acid and ethanol has been applied to a fructosamine assay using nitroblue tetrazolium. A fructosamine assay using extracted albumin sample was carried out without interference from low molecular weight substances with reducing activities and other proteins with varying concentrations, half-lives and reducing activities. 1-Deoxy-1-p-toluidino-D-fructose (DTF) was selected as a calibrator for the assay because it is a stable Amadori reaction product with a constant glycation rate. Albumin fructosamine value was calculated using the calibration curve of DTF. The corrected albumin fructosamine value was expressed as the amount of albumin fructosamine per gram of extracted albumin taking into consideration the variation in albumin concentrations in sera from patients. The corrected albumin fructosamine values correlated more closely with the fasting blood glucose levels (r‫)537.0؍‬ than the serum fructosamine values corrected for albumin concentrations (r‫)415.0؍‬ (pϽ0.05).

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Glycosylation of serum albumin: Elevated glycosyl‐albumin in diabetic patients

Cited by 168 publications

References 15 publications

Elevation of the glycated albumin to glycated hemoglobin ratio during the progression of hepatitis C virus related liver fibrosis

Elevation of the glycated albumin to glycated hemoglobin ratio during the progression of hepatitis C virus related liver fibrosis

Estimation of glycated hemoglobin by 2,6-dimethylphenol: Sulphuric acid conventional method

Fructosamine Assay Using Albumin Extracted from Serum.

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