Estimation of glycated hemoglobin by 2,6-dimethylphenol: Sulphuric acid conventional method

Abstract

Glycated hemoglobin levels in hemolysate of normal and diabetic patients were determined by the 2,6-dimnthylphenol:67.5% sulphudc acid conventional method and the values were 0.3930.025 and 0.5910.21 moles of hydroxymethylfurfural(HMF)/mole of globin, respectively. The mean increase in glyceted hemoglobin values in diabetics (1.8 fold) was highly significant (p<0.001). A good correlation (r--0.95) was found the glycated hemoglobin values obtained by this method and the phenol:sulphudc acid method. The values obtained by former method were about 1.2.1.4 times the values by the phenoi:sulphuric acid method. This study indicates that conventional 2,6-dimethylphenoh57.5% suiphuric acid method is more sensitive for the estimation of glycetsd hemoglobin than any other method based on the same principle, it is less time consuming, reliable and hence can be employed for the routine laboretoW estimation of glyceted hemoglobin for the assessment of glycemic control.

Enhancing sensitivity of phenol-sulfuric acid assay using orthophosphoric acid as modifier

“…1 It has also been employed for measurement of glycated haemoglobin. 2 Presence of any monosaccaride or oligosaccharide having a C-2 hydroxyl group can be detected by phenol-sulfuric acid method. 3 The principle is based on dehydration of sugar in acidic medium forming hydroxymethylfurfural and its condensation with phenol to colored complex that can be measured at 490 nm.…”