Glycosylation of gp116 and gp64 envelope proteins of yellow head virus of Penaeus monodon shrimp

Soowannayan, Chumporn; Cowley, Jeff A.; Pearson, Roger; Wallis, Tristan P.; Gorman, Jeffrey J.; Michalski, Wojtek P.; Walker, Peter

doi:10.1099/vir.0.022111-0

Cited by 11 publications

(7 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The YHV strain used in this study originated from moribund shrimp obtained in 1999 from a commercial shrimp farm in Chonburi, Thailand, where outbreaks of yellow head disease had occurred. Crude virus or virus inoculum was prepared as described earlier (Soowannayan et al, 2003(Soowannayan et al, , 2010. Briefly, haemolymph from an infected shrimp was drawn into a syringe containing an equal volume of shrimp salt solution (450 mM NaCl, 10 mM KCl, 10 mM EDTA, 10 mM HEPES), mixed thoroughly before haemocytes were pelleted by centrifugation at 2000 g and kept at 280 uC.…”

Section: Methodsmentioning

confidence: 99%

“…The filtrate was then aliquoted and stored at 280 uC for use as inoculum for experimental infections. This virus has been used in many studies in our laboratory since it was first isolated from infected shrimp (Soowannayan et al, 2002(Soowannayan et al, , 2003(Soowannayan et al, , 2010(Soowannayan et al, , 2011.…”

Section: Methodsmentioning

confidence: 99%

“…Carbohydrates attached to both glycoproteins have recently been characterized and all were found to be N -linked glycans (Soowannayan et al , 2010). Using a combination of mass spectrometry and enzyme digestion, it was found that most of the putative N -linked glycosylation sites of gp116 (6/7) and gp64 (3/4) were utilized.…”

Section: Introductionmentioning

confidence: 99%

“…The unutilized, putative N -linked site of gp64 was identified whilst that of gp116 was not. The carbohydrates or glycans attached to the gp64 and gp116 proteins were found to consist mostly of mannose but also a variety of other glycans (Soowannayan et al , 2010).…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

N-Linked glycosylation is essential for the yellow head virus replication cycle

Soowannayan

Chanarpakorn

Phanthura

et al. 2013

Journal of General Virology

View full text Add to dashboard Cite

Yellow head virus (YHV) particles contain a nucleocapsid protein (p20) and two envelope glycoproteins (gp116 and gp64). The glycans attached to the two glycoproteins are N-linked and are complex and high mannose types, respectively. Here, we show that treatment with the N-linked glycosylation inhibitor tunicamycin in YHV-infected black tiger shrimp (Penaeus monodon) resulted in less severe yellow head disease and reduced mortality when compared with untreated control shrimp. Quantitative real-time reverse transcription PCR analysis also revealed lower YHV copy numbers in the haemolymph of treated than control shrimp. This was concurrent with less intense immuno-reactions in tissues of treated versus untreated shrimp using mAbs against all three YHV structural proteins. In addition, transmission electron microscopy of lymphoid organ tissue of the treated and untreated shrimp [eight collected at 36 h and eight at 48 h post-infection (p.i.)] revealed only unenveloped nucleocapsids in all but one of the treated shrimp (collected at 48 h p.i.). By contrast, all the untreated shrimp showed a mixture of many unenveloped and enveloped virions. These results were supported by purification of YHV from the cell-free haemolymph of treated and untreated shrimp followed by YHV structural protein analysis by SDS-PAGE. It revealed three expected structural protein bands (116, 64 and 20 kDa) from the untreated shrimp but no structural protein bands from the tunicamycin-treated shrimp (confirmed by Western blot analysis). Overall, the results indicated that blocking glycosylation with tunicamycin inhibited the formation of mature YHV virions and their subsequent release into shrimp haemolymph, reducing the severity of disease.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

N-Linked glycosylation is essential for the yellow head virus replication cycle

Soowannayan

Chanarpakorn

Phanthura

et al. 2013

Journal of General Virology

View full text Add to dashboard Cite

show abstract

“…The most common method for detecting site occupancy is to utilize the conversion of the Asn to which the N ‐glycans are attached to aspartic acid (Asp) when the glycans are released with PNGase F. The increase by one mass unit is readily detected by MS. The method has been used, for example to confirm occupancy of six of the seven potential N ‐linked glycosylation sites in the envelope glycoprotein gp116 and three of the four potential sites in the gp64 protein of the yellow head virus from the Penaeus monodon shrimp (Soowannayan et al, ).…”

Section: Studies On Specific Carbohydrate Typesmentioning

confidence: 99%

Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2009–2010

Harvey

2014

Mass Spectrometry Reviews

View full text Add to dashboard Cite

This review is the sixth update of the original article published in 1999 on the application of MALDI mass spectrometry to the analysis of carbohydrates and glycoconjugates and brings coverage of the literature to the end of 2010. General aspects such as theory of the MALDI process, matrices, derivatization, MALDI imaging, arrays and fragmentation are covered in the first part of the review and applications to various structural typed constitutes the remainder. The main groups of compound that are discussed in this section are oligo and polysaccharides, glycoproteins, glycolipids, glycosides and biopharmaceuticals. Many of these applications are presented in tabular form. Also discussed are medical and industrial applications of the technique, studies of enzyme reactions and applications to chemical synthesis.

show abstract

Yellow head viruses and disease

Soowannayan

Dong

2022

Aquaculture Pathophysiology

View full text Add to dashboard Cite

Glycosylation of gp116 and gp64 envelope proteins of yellow head virus of Penaeus monodon shrimp

Cited by 11 publications

References 55 publications

N-Linked glycosylation is essential for the yellow head virus replication cycle

N-Linked glycosylation is essential for the yellow head virus replication cycle

Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2009–2010

Yellow head viruses and disease

Contact Info

Product

Resources

About