Glycosylated haemoglobin in the fetus: Chemistry, laboratory measurements and future clinical implications

Weissman, Ariel; Hagay, Zion

doi:10.1002/dmr.5610100105

Cited by 6 publications

(1 citation statement)

References 89 publications

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“…Hemoglobin A1c (HbA1c) is an indicator of irreversible glycosylation of the NH2-terminus of the beta chain of adult hemoglobin (HbA), and is widely used to assess glycemic control in diabetic children and adults [10]. HbA1c is the major fraction of total glycosylated hemoglobin (GlyHb) and is a measure of red cell glucose exposure over the previous two to three months.…”

Section: Introductionmentioning

confidence: 99%

Development of filter paper hemoglobin A1c assay applicable to newborn screening

Pollock

Allen

Wiebe

et al. 2016

Clinica Chimica Acta

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Purpose Gestational diabetes influences risk for future metabolic disease including type 2 diabetes. Hemoglobin A1c (HbA1c) measurement assesses hemoglobin A glycosylation, and could theoretically be used as a test to estimate gestational glucose exposure. HbA1c assay on dried blood spots (DBS) is needed before potential application to statewide newborn screening (NBS) population studies. The study aimed to establish a reliable method to measure HbA1c on NBS DBS specimens. De-identified blood was used to generate trials to evaluate stability of HbA1c in DBS, optimal elution time, and stability of eluted blood. Results Analysis of DBS stability HbA1c measurements from 3-6 days after collection overestimated HbA1c values by a bias factor between 0.83 and 0.87. Sixty minutes of elution time produced maximal reproducibility and minimal bias of results. Within assay standard deviation: 0.058; average bias: -0.02%. Stability of eluted blood did not vary significantly between days 0-2 after DBS elution. Conclusions Measurement of HbA1c levels on DBS from human blood is feasible. Results suggest new method using DBS to measure HbA1c level with the following characteristics: optimal time for sample analysis 3-6 days after collection, elution time of 60 minutes and eluted blood analysis within 2 days of elution. Measurement of neonatal HbA1c could provide insight regarding the infant's in utero exposure to glucose.

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Section: Introductionmentioning

confidence: 99%

Development of filter paper hemoglobin A1c assay applicable to newborn screening

Pollock

Allen

Wiebe

et al. 2016

Clinica Chimica Acta

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Derivated fetal haemoglobin as a marker for red cell age in the human fetus reflecting stimulated or impaired red blood cell production

2001

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We have determined whether derivated fetal haemoglobin (dHbF, consisting of glycated and acetylated HbF) can be used as a cell age marker for fetal red blood cells (RBCs). Cord blood was obtained between 19 and 39 weeks of gestation from 28 alloimmunised anaemic fetuses (23 RhD+ and 5 Kell) and from 20 non-anaemic fetuses and newborns (controls). Density gradient centrifugation was applied to 36 samples (20 RhD+, 15 controls and 1 Kell) to obtain fractions of increasing cell age. Blood samples were used for measurements of mean cellular volume (MCV), mean cell haemoglobin (MCH), mean corpuscular haemoglobin concentration (MCHC), pyruvate kinase activity (PK) and derivated fetal haemoglobin (dHbF) by cation-exchange HPLC. Reticulocytes were counted only in the whole blood samples. In all density gradient separated RBC fractions, the values for MCV, MCH and PK activity decreased and those of MCHC and dHbF increased with increasing density (equivalent to increasing cell age). The mean density was lower for RBCs of the anaemic RHD group (1.072+/-0.007 g/ml) than for the non-anaemic controls (1.077+/-0.005 g/ml) (p<0.05) The RBC density of the Kell sensitised fetus did not differ from those of the controls. In the control group, the values of the cell age markers in whole blood changed significantly with the gestational age, showing an increase of mean age of the erythrocyte population. The best linear relationship was found for dHbF (y=6.28+0.17*weeks; r=0.84; p<0.001). In the anaemic RhD+ fetuses, the RBC age markers did not change with gestational age; the dHbF percentages were lower, and the MCV, MCH, PK values and the reticulocyte counts were higher than in the controls (0.05

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Diabetes in pregnancy: Effect on glycation and acetylation of the different chains of fetal and maternal hemoglobin

Davison¹,

Green²,

Roberts³

2011

Clinical Biochemistry

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Glycosylated haemoglobin in the fetus: Chemistry, laboratory measurements and future clinical implications

Cited by 6 publications

References 89 publications

Development of filter paper hemoglobin A1c assay applicable to newborn screening

Development of filter paper hemoglobin A1c assay applicable to newborn screening

Derivated fetal haemoglobin as a marker for red cell age in the human fetus reflecting stimulated or impaired red blood cell production

Diabetes in pregnancy: Effect on glycation and acetylation of the different chains of fetal and maternal hemoglobin

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