Glycosphingolipid carriers of carbohydrate antigens of human myeloid cells recognized by monoclonal antibodies

Uemura, K.; Macher, Bruce A.; DeGregorio, Michael W.; Scudder, Peter; Buehler, Joanne; Knapp, Walter; Feizi, Ten

doi:10.1016/0167-4889(85)90106-5

Cited by 22 publications

(6 citation statements)

References 47 publications

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“…8). 65 Overall, ABH‐active neutral GSLs constitute less than 1 percent of the total neutral GSL on platelets, numbering approximately 5000 molecules per platelet 24,53 …”

Section: Discussionmentioning

confidence: 99%

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Human platelets express gangliosides with LKEactivity and ABH blood group activity

2001

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“…8). 65 Overall, ABH‐active neutral GSLs constitute less than 1 percent of the total neutral GSL on platelets, numbering approximately 5000 molecules per platelet 24,53 …”

Section: Discussionmentioning

confidence: 99%

“…5). 21,22,24,35 In contrast, RBCs express little GM3 and do not express sLe x or VIM‐active gangliosides 24,35,65 . RBCs express GD3 (50‐60% total) and related neolacto‐family gangliosides with sialyl‐i, sialyl‐I, and sialyl‐I/ABH activity 2,66,67 .…”

Section: Discussionmentioning

confidence: 99%

Human platelets express gangliosides with LKEactivity and ABH blood group activity

2001

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“…Monocytes and macrophages seem to have a different neutral GSL composition compared to other human myeloid immune cells since they express globosides ((iso)Gb3 and Gb4) as the major neutral GSLs (36, 44, 45, 48, 52). Neolacto-series GSLs such as Lc3 and nLc4 are also detectable and upregulated during differentiation toward moDCs, but are reduced during differentiation toward macrophages as a result of decreased B3GNT5 gene expression (36, 44, 45, 55, 96). Additionally, during macrophage differentiation the expression of Gb5 is upregulated, which—like Gb3—is a target for the human immunodeficiency virus (HIV) gp120 glycoprotein (94, 99).…”

Section: Biosynthesis and Expression Of Gsls In Naïve And Differentiamentioning

confidence: 99%

The Role of Glycosphingolipids in Immune Cell Functions

et al. 2019

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Glycosphingolipids (GSLs) exhibit a variety of functions in cellular differentiation and interaction. Also, they are known to play a role as receptors in pathogen invasion. A less well-explored feature is the role of GSLs in immune cell function which is the subject of this review article. Here we summarize knowledge on GSL expression patterns in different immune cells. We review the changes in GSL expression during immune cell development and differentiation, maturation, and activation. Furthermore, we review how immune cell GSLs impact membrane organization, molecular signaling, and trans-interactions in cellular cross-talk. Another aspect covered is the role of GSLs as targets of antibody-based immunity in cancer. We expect that recent advances in analytical and genome editing technologies will help in the coming years to further our knowledge on the role of GSLs as modulators of immune cell function.

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“…Sphingolipids (SLs) are bioactive lipids asymmetrically distributed at the external leaflet of the plasma membrane (PM) of all eukaryotic cells. They are characterized by a hydrophilic head group protruding toward the extracellular environment and by ceramide (Cer) a peculiar lipid moiety, deeply inserted into the membrane bilayer [ 1 ]. Cer is composed by a long chain base, the sphingosine, linked to a fatty acid of different length and saturation.…”

Section: Introductionmentioning

confidence: 99%

Sphingolipids and plasma membrane hydrolases in human primary bronchial cells during differentiation and their altered patterns in cystic fibrosis

et al. 2020

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Human primary bronchial epithelial cells differentiated in vitro represent a valuable tool to study lung diseases such as cystic fibrosis (CF), an inherited disorder caused by mutations in the gene coding for the Cystic Fibrosis Transmembrane Conductance Regulator. In CF, sphingolipids, a ubiquitous class of bioactive lipids mainly associated with the outer layer of the plasma membrane, seem to play a crucial role in the establishment of the severe lung complications. Nevertheless, no information on the involvement of sphingolipids and their metabolism in the differentiation of primary bronchial epithelial cells are available so far. Here we show that ceramide and globotriaosylceramide increased during cell differentiation, whereas glucosylceramide and gangliosides content decreased. In addition, we found that apical plasma membrane of differentiated bronchial cells is characterized by a higher content of sphingolipids in comparison to the other cell membranes and that activity of sphingolipids catabolic enzymes associated with this membrane results altered with respect to the total cell activities. In particular, the apical membrane of CF cells was characterized by high levels of ceramide and glucosylceramide, known to have proinflammatory activity. On this basis, our data further support the role of sphingolipids in the onset of CF lung pathology.

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Glycosphingolipid carriers of carbohydrate antigens of human myeloid cells recognized by monoclonal antibodies

Cited by 22 publications

References 47 publications

Human platelets express gangliosides with LKEactivity and ABH blood group activity

Human platelets express gangliosides with LKEactivity and ABH blood group activity

The Role of Glycosphingolipids in Immune Cell Functions

Sphingolipids and plasma membrane hydrolases in human primary bronchial cells during differentiation and their altered patterns in cystic fibrosis

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