Glycosides and flavonoids from the extract of <i>Pueraria thomsonii</i> Benth leaf alleviate type 2 diabetes in high-fat diet plus streptozotocin-induced mice by modulating the gut microbiota

Zhang, Shanshan; Zhang, Niu-Niu; Guo, Shuxiang; Liu, Shaojing; Hou, Yufei; Li, Shiming; Ho, Chi‐Tang; Bai, Naisheng

doi:10.1039/d1fo04170c

Cited by 16 publications

(8 citation statements)

References 66 publications

(94 reference statements)

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“…The IC 50 value of 0.506 ± 0.001 mM for compound AF−15 (kaempferol−4’,7−dimethyl−3−O−glucoside), in which the two hydroxyl hydrogens at the 4’ and 7 positions were substituted with methyl groups and the hydroxyl hydrogens at the 3 position was substituted with glucose groups, which had the smallest IC 50 among the 12 flavonoids indicating that the α−glucosidase inhibitory activity of AF−15 was the lowest among these 12 flavonoids. The above results suggested that the reduction of phenolic hydroxyl groups in the B and C rings of the flavonoid backbone and the occurrence of glucosyl substitution led to the reduction of α−glucosidase inhibitory activity, which was consistent with the results of previous studies [ 24 , 26 ].…”

Section: Resultssupporting

confidence: 92%

“…The α−glucosidase inhibitory activity assay method of A. tenuissimum flower flavonoids was slightly modified based on previous studies [ 22 , 23 , 24 ]. A 96−well plate was used for preparing the mixture consisting of 0.1 M PBS (pH 6.8), 1.0 mg/mL sample solution, and 2 U/mL α−glucosidase, which were incubated at 37 °C for 10 min.…”

Section: Methodsmentioning

confidence: 99%

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Exploring Active Ingredients, Beneficial Effects, and Potential Mechanism of Allium tenuissimum L. Flower for Treating T2DM Mice Based on Network Pharmacology and Gut Microbiota

et al. 2022

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Forty compounds were isolated and characterized from A. tenuissimum flower. Among them, twelve flavonoids showed higher α−glucosidase inhibition activities in vitro than acarbose, especially kaempferol. The molecular docking results showed that the binding of kaempferol to α−glucosidase (GAA) could reduce the hydrolysis of substrates by GAA and reduce the glucose produced by hydrolysis, thus exhibiting α−glucosidase inhibition activities. The in vivo experiment results showed that flavonoids−rich A. tenuissimum flower could decrease blood glucose and reduce lipid accumulation. The protein expression levels of RAC−alpha serine/threonine−protein kinase (AKT1), peroxisome proliferator activated receptor gamma (PPARG), and prostaglandin G/H synthase 2 (PTGS2) in liver tissue were increased. In addition, the Firmicutes/Bacteroidetes (F/B) ratio was increased, the level of gut probiotics Bifidobacterium was increased, and the levels of Enterobacteriaceae and Staphylococcus were decreased. The carbohydrate metabolism, lipid metabolism, and other pathways related to type 2 diabetes mellitus were activated. This study indicating flavonoids−rich A. tenuissimum flower could improve glycolipid metabolic disorders and inflammation in diabetic mice by modulating the protein expression and gut microbiota.

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Section: Resultssupporting

confidence: 92%

Section: Methodsmentioning

confidence: 99%

Exploring Active Ingredients, Beneficial Effects, and Potential Mechanism of Allium tenuissimum L. Flower for Treating T2DM Mice Based on Network Pharmacology and Gut Microbiota

et al. 2022

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“… 35 Pueraria lobata extract improved glucose and lipid metabolism and inflammation by regulating GM and treating T2DM. 36 HAW1-2 in hawthorn directly altered the intestinal microflora (particularly Bacteroides ) and produced SCFAs. 37 Eucommia ulmoides balanced the structure and composition of intestinal microflora and improved intestinal tissue morphology.…”

Section: Discussionmentioning

confidence: 99%

Tang-Ping-San Decoction Remodel Intestinal Flora and Barrier to Ameliorate Type 2 Diabetes Mellitus in Rodent Model

Yin

Zhang

Pang

et al. 2022

DMSO

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Purpose Type 2 diabetes mellitus (T2DM) is a complex genetic disease associated with genetic and environmental factors. Previous studies have shown that changes in the gut microbiota may affect the development of host metabolic diseases and promote the progression of T2DM. Tang-ping-san (TPS) decoction can effectively treat T2DM. However, its specific mechanisms must be evaluated. Patients and Methods In the present study, we established an animal model of T2DM using a high‑fat diet (HFD) with intraperitoneal injection streptozotocin injection. Results The therapeutic effect of TPS decoction on T2DM in mice was initially evaluated. TPS decoction was found to improve hyperglycemia, hyperlipidemia, insulin resistance, and pathological liver, pancreatic, and colon changes. Moreover, it reduced the pro-inflammatory cytokine levels. Based on 16SrRNA sequencing, TPS decoction reduced the Firmicutes/Bacteroidetes ratio at the phylum level. At the genus level, it increased the relative abundances of Akkermansia, Muribaculaceae , and the Eubacterium coprostanoligenes group and decreased the relative abundance of Fusobacterium, Escherichia coli, Dubosiella , and Helicobacter . Conclusion TPS decoction improves T2DM and liver function and reduces the risk of hyperglycemia, hyperlipidemia, insulin resistance, pathological organ changes, and inflammatory reactions. The mechanism of TPS decoction in T2DM can be correlated with the reversal of gut microbiota dysfunction and repair of the intestinal mucosal barrier.

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“…The gut microbiota play essential roles in the digestion and absorption of flavonoids and affect the occurrence and progression of T2DM. Flavonoids effectively increased insulin levels, decreased FBG content, reduced lipid accumulation in plasma, alleviated oxidative injury and inflammation, and relieved liver and kidney damage in diabetic mice ( 50 ). For example, Pueraria extract contains nine flavonoids; they can increase intestinal probiotics to improve metabolic disorders caused by diabetes and decrease Clostridium celatum levels to alleviate inflammation.…”

Section: Effects Of Chinese Herb Ingredients On Intestinal Microbiotamentioning

confidence: 99%

“…For example, Pueraria extract contains nine flavonoids; they can increase intestinal probiotics to improve metabolic disorders caused by diabetes and decrease Clostridium celatum levels to alleviate inflammation. Flavonoids have the potential to be used to control type 2 diabetes by regulating glycolipid metabolism and inflammation levels ( 50 , 51 ).…”

Section: Effects Of Chinese Herb Ingredients On Intestinal Microbiotamentioning

confidence: 99%

Review of the correlation between Chinese medicine and intestinal microbiota on the efficacy of diabetes mellitus

Min

Tang

et al. 2023

Front. Endocrinol.

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Diabetes mellitus is a serious metabolic disorder that can lead to a number of life-threatening complications. Studies have shown that intestinal microbiota is closely related to the development of diabetes, making it a potential target for the treatment of diabetes. In recent years, research on the active ingredients of traditional Chinese medicine (TCM), TCM compounds, and prepared Chinese medicines to regulate intestinal microbiota and improve the symptoms of diabetes mellitus is very extensive. We focus on the research progress of TCM active ingredients, herbal compounds, and prepared Chinese medicines in the treatment of diabetes mellitus in this paper. When diabetes occurs, changes in the abundance and function of the intestinal microbiota disrupt the intestinal environment by disrupting the intestinal barrier and fermentation. TCM and its components can increase the abundance of beneficial bacteria while decreasing the abundance of harmful bacteria, regulate the concentration of microbial metabolites, improve insulin sensitivity, regulate lipid metabolism and blood glucose, and reduce inflammation. TCM can be converted into active substances with pharmacological effects by intestinal microbiota, and these active substances can reverse intestinal microecological disorders and improve diabetes symptoms. This can be used as a reference for diabetes prevention and treatment.

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Glycosides and flavonoids from the extract of Pueraria thomsonii Benth leaf alleviate type 2 diabetes in high-fat diet plus streptozotocin-induced mice by modulating the gut microbiota

Abstract: Twenty glycoside derivatives and nine flavonoids from the leaves of Pueraria (P. thomsonii) were isolated by column chromatography and characterized by nuclear magnetic resonance spectroscopy (NMR) and high performance liquid...

Cited by 16 publications

References 66 publications

Exploring Active Ingredients, Beneficial Effects, and Potential Mechanism of Allium tenuissimum L. Flower for Treating T2DM Mice Based on Network Pharmacology and Gut Microbiota

Exploring Active Ingredients, Beneficial Effects, and Potential Mechanism of Allium tenuissimum L. Flower for Treating T2DM Mice Based on Network Pharmacology and Gut Microbiota

Tang-Ping-San Decoction Remodel Intestinal Flora and Barrier to Ameliorate Type 2 Diabetes Mellitus in Rodent Model

Review of the correlation between Chinese medicine and intestinal microbiota on the efficacy of diabetes mellitus

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