Glycosidases and B group vitamins produced by six yeast strains from the digestive tract of Phoracantha semipunctata larvae and their role in the insect development

Chararas, C.; Pignal, Marie Claire; Vodjdani, Guilane; Bourgeay-Causse, M

doi:10.1007/bf00437405

Cited by 13 publications

(6 citation statements)

References 8 publications

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“…Cruden & Markovetz, 1987; Kaufman et al ., 1989; Breznak & Brune, 1994) and are involved in methanogenesis and nitrogen fixation (Hackstein & Stumm, 1994; Nardi et al ., 2002). The gut microflora is also implicated in pheromone production (Byers & Wood, 1981; Dillon et al ., 2000), vitamin synthesis (Chararas et al ., 1983), pesticide degradation (Boush & Matsumura, 1967), and there is evidence that gut bacteria can prevent the growth of some insect pathogens (Dillon & Charnley, 1995). As well as making use of bacteria in the gut lumen, it has been estimated that around 55 000 insect species harbour endosymbiotic bacteria within specialized host cells; these bacteria provision their hosts with nutrients that are often lacking from their diets (Wilkinson, 2001).…”

Section: Introductionmentioning

confidence: 99%

Application of 16S rDNA‐DGGE to examine the microbial ecology associated with a social wasp Vespula germanica

Reeson¹,

Jankovič²,

Kasper

et al. 2003

Insect Molecular Biology

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Invertebrates host numerous bacteria, with interactions ranging from pathogenesis to symbiosis. While certain symbiotic relationships have been well studied, little is known about the dynamics of these bacterial communities. Denaturing gradient gel electrophoresis (DGGE) was used to examine the bacterial microflora associated with the eusocial wasp Vespula germanica. DGGE profiles of larval guts revealed a variable microflora, suggesting that V. germanica is not dependent on a particular suite of mutualists. The variation in profiles was not related to season, nest size or macrohabitat. Sequences corresponding to Lactococcus, Lactobacillus, a novel Leuconostoc and two Rickettsiella grylli strains were obtained. DGGE proved to be a useful technique for characterizing the wasp microflora. Given the importance of microbial communities to invertebrates, there is much to be gained from the application of such techniques.

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Section: Introductionmentioning

confidence: 99%

Application of 16S rDNA‐DGGE to examine the microbial ecology associated with a social wasp Vespula germanica

Reeson¹,

Jankovič²,

Kasper

et al. 2003

Insect Molecular Biology

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“…It should be noted that Chararas and Pignal (1981) demonstrated that the cultures of two yeasts, isolated from the whole gut (wall + contents) of P. semipunctata larvae and identified as Candida guilliermondii and C. tenuis, were able to degrade neither carboxymethylcellulose nor swollen cellulose. Moreover, cultures of other isolated yeasts (c. intermedia, C. diddensii, Toru/opsis molischiana) did not exhibit any activity against swollen cellulose (Chararas et al 1983).…”

Section: Discussionmentioning

confidence: 92%

Studies on the Digestion of Cellulose by the Larvae of the Eucalyptus Borer, Phoracantha semipunctata (Coleoptera: Cerambycidae)

Chararas¹,

Chipoulet²

1983

Aust. Jnl. Of Bio. Sci.

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Larvae of the native Australian xylophagous insect P. semipunctata, the adults of which lay eggs only on Eucalyptus species, penetrate the bark, then the phloem and feed at the boundary between the phloem and the xylem, causing deep furrows. As the phloem dries, they penetrate the xylem, which they damage by the formation of galleries. Digestion of wood is facilitated by glycosidases acting not only on oligosaccharides but also on many polysaccharides, notably starch, various hemicelluloses and cellulose. The role of a cellulase produced by the midgut epithelium of the larvae was examined and the characteristics of a partially purified preparation of this enzyme from the midgut epithelium studied. The enzyme was shown to have a molecular weight of less than 34500 and to hydrolyse carboxymethylcellulose and Cellulose-Azure: It was identified as being an endo-ft-l,4-glucanase.

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“…Many authors hypothesize that filamentous fungi could be a nutritional source for beetles, mainly by providing nitrogen and sterols, essential for oogenesis and larval development (Klepzig & Six, 2004; Bentz & Six, 2006; Adams & Six, 2007). Moreover, Candida species isolated from Coleoptera degrade many compounds, such as sugar and cellulose, and produce vitamins (Chararas et al ., 1983). More recently, the implication of associated yeasts in beetle nutrition has been hypothesized (Davis, 2014; Durand et al ., 2017).…”

Section: Discussionmentioning

confidence: 99%

The symbiotic complex of Dendroctonus simplex: implications in the beetle attack and its life cycle

Durand

Constant

Déziel

et al. 2019

Bull. Entomol. Res.

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3 4 5 6 7 8 9 10 11 12 13 14 15 16 Figure S1. Principal component analysis of the different developmental stages and microenvironments of D. simplex for bacterial and fungal 17 microbiota. All generated OTU and equalized dataset has been used to generate the PCA. Numbers in parenthesis represent the percentage 18 explained by each axes. ECTO = ectomicrobiota, ENDO = endomicrobiota, ADU = adults, LAR = Larvae, PUP = Pupae. 19 −0.6 −0.4 −0.2 0.0 0.2 0.4 0.6 −0.4 −0.2 0.0 0.2 0.4 Bacteria PC1 (20.4%) PC2 (7.8%) −0.6 −0.4 −0.2 0.0 0.2 0.4 0.6 −0.4 −0.2 0.0 0.2 0.4

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Glycosidases and B group vitamins produced by six yeast strains from the digestive tract of Phoracantha semipunctata larvae and their role in the insect development

Cited by 13 publications

References 8 publications

Application of 16S rDNA‐DGGE to examine the microbial ecology associated with a social wasp Vespula germanica

Application of 16S rDNA‐DGGE to examine the microbial ecology associated with a social wasp Vespula germanica

Studies on the Digestion of Cellulose by the Larvae of the Eucalyptus Borer, Phoracantha semipunctata (Coleoptera: Cerambycidae)

The symbiotic complex of Dendroctonus simplex: implications in the beetle attack and its life cycle

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