Application of 16S rDNA‐DGGE to examine the microbial ecology associated with a social wasp <i>Vespula germanica</i>

Reeson, Andrew; Jankovič, T.; Kasper, Marta; Rogers, S. L.; Austin, Andrew D.

doi:10.1046/j.1365-2583.2003.00390.x

Cited by 92 publications

(85 citation statements)

References 42 publications

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“…Most of the data obtained on insect gut bacterial diversity is derived from traditional microbial methods such as viable plate counts and phenetic taxonomy. However, in recent years the use of the rRNA approach enables further characterization of the enteric microbiota of many insects (Egert et al, 2003;Reeson et al, 2003;Dillon and Dillon, 2004;Behar et al, 2005;Capuzzo et al, 2005;Mohr and Tebbe, 2006).…”

Section: Introductionmentioning

confidence: 99%

“…Denaturing gradient gel electrophoresis (DGGE) allows the resolution of DNA fragments differing by as little as a single base pair. Coupled with PCR, this method has been applied to environmental studies (Muyzer et al, 1993), and has been successfully implemented in the study of bacterial communities associated with insects (Reeson et al, 2003;Behar et al, 2005;Behar et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

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Community Structure of the Mediterranean Fruit Fly Microbiota: Seasonal and Spatial Sources of Variation

Behar

Yuval

Jurkevitch

2008

Israel Journal of Ecology & Evolution

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Fruit flies (Diptera: Tephritidae) maintain diverse yet stable communities of Enterobacteriaceae that reside in their digestive system. Focusing on one species of this family, Ceratitis capitata, the Mediterranean fruit fly (medfly), we applied the PCR-DGGE approach to study the influence of temporal and spatial variations on the composition and diversity of the enterobacterial community associated with the medfly. Our results revealed that the Enterobacteriaceae constituted not only dominant, but also constant and stable populations in the medfly's gut. Nevertheless, changes in both time and space had an impact on the structure and diversity of these bacterial populations, and while some populations (e.g., Klebsiella spp.) were stable, others fluctuated. Whether the bacterial population fluctuated according to the ecological needs of the insect during different environmental conditions, or reflected opportunistic colonization abilities of some bacterial strains, remains to be determined.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Community Structure of the Mediterranean Fruit Fly Microbiota: Seasonal and Spatial Sources of Variation

Behar

Yuval

Jurkevitch

2008

Israel Journal of Ecology & Evolution

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“…The insect's gut is represented by a large source of as yet unexplored microbial diversity that play important roles from utilization of different organic polymers, methanogenesis, nitrogen fixation to pheromone production, pesticide degradation and pathogen prevention [17][18][19]. Data on the different insect gut microbiome and their functional aspects is a vast area that require more scientific inputs [19].…”

Section: Diversity and Role Of Insect Gut Microbes And Their Environmmentioning

confidence: 99%

Health and environmental applications of gut microbiome: a review

Chatterjee

Datta

Sharma

et al. 2017

Ecological Chemistry and Engineering S

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Life on Earth harbours an unimaginable diversity of microbial communities. Among these, gut microbiome, the ecological communities of commensal, symbionts (bacteria and bacteriophages) are a unique assemblage of microbes. This microbial population of animal gut helps in performing organism's physiological processes to stay healthy and fit. The role of these microbial communities is immense. They continually maintain interrelation with the intestinal mucosa in a subtle equilibrium and help the gut for different functions ranging from metabolism to immunologic functions like upgradation of nutrient-poor diets, aid in digestion of recalcitrant food components, protection from pathogens, contribute to inter-and intra-specific communication, affecting the efficiency as disease vectors etc. The microbial diversity in the gut depends upon environmental competition between microbes, their sieving effects and subsequent elimination. Due to wide diversity of anatomy and physiology of the digestive tracts and food habits, the gut microbiome also differs broadly among animals. Stochastic factors through the history of colonization of the microbiome in a species and in situ evolution are likely to establish interspecies diversity. Moreover, the microbes offer enormous opportunity to discover novel species for therapeutic and/or biotechnological applications. In this manuscript, we review the available knowledge on gut microbiome, emphasising their role in health and health related applications in human.

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“…미생물생태학의 연구는 군집구조 연구를 위한 molecular fingerprinting기술을 제공하는 denaturing gradient gel electrophoresis (DGGE)와 temperature gradient gel electrophoresis (TGGE)의 도입으로 크나 큰 발전을 가져왔다 [8]. DGGE분석에서 16S 또는 28S rRNA gene의 크기는 동일 하나 서열이 다른 PCR산물을 분리할 수 있었으며 이 기술은 토양 [28], 해양 [2], 곤충 [27], sludge [35] 등 다양한 환경에서 미생물의 dynamics 연구를 위해 광범위하게 적용되어 왔다. 또한 이러한 방법은 누룩 [17], 김치 [3], 와인 [5,22], sausage [26], sourdough [24], coffee [23], 치즈 [6] (Fig.…”

Section: 서 론unclassified

Analysis of Microbial Diversity in Makgeolli Fermentation Using PCR-DGGE

Kwon¹,

Ahn²,

Sohn³

2012

Journal of Life Science

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Kumjungsansung-Makgeolli® is a traditional Korean rice wine that is fermented from traditional nuruk and rice. In this study, we performed the PCR-denaturing gradient gel electrophoresis (DGGE) analysis targeting the 16S and 28S rRNA genes to characterize bacterial and fungal diversity during Makgeolli fermentation. The predominant bacteria in the PCR-DGGE profile during Makgeolli fermentation were Lactobacillus spp. (Lactobacillus curvatus, L. kisonensis, L. plantarum, L. sakei, and L. gasseri), Pediococcus spp. (P. acidilactici, P. parvulus, P. agglomerans, and P. pentosaceus), Pantoea spp. (P. agglomerans and P. ananatis), and Citrobacter freundii; these were identified on the base of analysis of 16S rRNA gene sequences. The dominant bacterium during Makgeolli fermentation was L. curvatus. The predominant fungi in PCR-DGGE profile during Makgeolli fermentation were Pichia kudriavzevii, Saccharomyces cerevisiae, Asidia idahoensis, Kluyveromyces marxianus, Saccharomycopsis fibuligera, and Torulaspora delbrueckii, and these were identified on the basis of analysis of 28S rRNA gene sequences. The dominant fungal species during Makgeolli fermentation changed from P. kudriavzevii at 0-2 days incubation to S. cerevisiae at 3-6 days incubation. This study suggests that PCR-DGGE analysis could be a suitable tool for the understanding of microbial diversity and structure during Makgeolli fermentation.

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Application of 16S rDNA‐DGGE to examine the microbial ecology associated with a social wasp Vespula germanica

Cited by 92 publications

References 42 publications

Community Structure of the Mediterranean Fruit Fly Microbiota: Seasonal and Spatial Sources of Variation

Community Structure of the Mediterranean Fruit Fly Microbiota: Seasonal and Spatial Sources of Variation

Health and environmental applications of gut microbiome: a review

Analysis of Microbial Diversity in Makgeolli Fermentation Using PCR-DGGE

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