Glycosaminoglycan Quality Control by Monosaccharide Analysis

Zhang, Yiran; Ma, Xuexiao; Zhang, Lijuan

doi:10.1007/978-1-0716-1398-6_24

Cited by 2 publications

(2 citation statements)

References 21 publications

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“…The obtained supernatant was transferred to a 500 mL conical flask, mixed evenly after adding 95% ethanol (the volume ratio of supernatant to ethanol was 1:4), stored in a refrigerator at 4 °C overnight, and then centrifuged to discard the ethanol and obtain the crude polysaccharide of HSP. The polysaccharides were purified by DEAE-52 and Sephadex gel (Sephadex G---100) columns following the method published by (Zhang et al, 2022c).…”

Section: Extraction and Purification Of Hspmentioning

confidence: 99%

“…The monosaccharide standards selected included galacturonic acid (Galua), glucose (GLC), galactose (Gal), arabinose (ARA), mannose (man), xylose (Xyl), rhamnose (RHA), glucuronic acid (GlcUA), and fucose (Fuc). According to the methods published, followed by HPLC analysis (Yao et al, 2020;Zhang et al, 2022c). Standard monosaccharides were subjected to the same conditions described above as a reference.…”

Section: Monosaccharide Composition Analysismentioning

confidence: 99%

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Preparation and application of carboxymethylated and phosphatised Melaleuca polysaccharide

Yan

Yuan

et al. 2023

Food Sci. Technol

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Polysaccharides are a class of biological macromolecules made up of sugar chains with multiple glycosidic bonds. To explore the extracted, purified, carboxymethylated and phosphorylated modified to obtained carboxymethylated polysaccharides (HSPS) and phosphorylated polysaccharides (HSPP), and the HSPS and HSPP were characterized and analyzed, and their antioxidant activity measured. Response surface design was used for the synthesis process of HSPS and HSPP, the structure characterization and antioxidant activities of HSPS and HSPP were evaluated. The results showed that the Mw and Mn of HSP were 14.66 kDa and 8.39 kDa, respectively. The monosaccharides were mainly galactose, glucose, xylose, and arabinose in a molar ratio of 37.68%: 24.43%: 13.63%: 9.18%. The optimal synthesis conditions for HSPS were as follows: reaction time of 2.01 h, reaction temperature of 44.61°C and the amount of monochloroacetic acid necessary was 1.18 g. The optimal synthesis conditions for HSPP were: reaction time of 4.28 h, reaction temperature of 80.78°C, and reaction pH of 8.98. HSPS and HSPP have scavenging effects on ABTS, super oxideanion and hydroxyl radicals. The HSPS and HSPP were characterized and analyzed, and their antioxidant activity measured.

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Section: Extraction and Purification Of Hspmentioning

confidence: 99%

Section: Monosaccharide Composition Analysismentioning

confidence: 99%